Ca2+/Calmodulin-dependent Protein Kinase IV Is Expressed in Spermatids and Targeted to Chromatin and the Nuclear Matrix

Wu, Joy Y.; Means, Anthony R.

doi:10.1074/jbc.275.11.7994

Cited by 59 publications

(50 citation statements)

References 40 publications

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“…However, it is known that protamines are phosphorylated before binding to DNA and that substantial dephosphorylation takes place concomitant with nucleoprotamine maturation. Indeed, mutation of the calmodulin-dependent protein kinase Camk4 that phosphorylates protamine 2, results in defective spermiogenesis and male infertility [164]. At fertilization, the mature sperm cells are packaged densely with protamines, whereas maternal genome is packaged with histones.…”

Section: Epigenetic Modifications and Male Infertilitymentioning

confidence: 99%

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Tahmasbpour

Balasubramanian

Agarwal

2014

J Assist Reprod Genet

109

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Background The assisted reproductive techniques aimed to assist infertile couples have their own offspring carry significant risks of passing on molecular defects to next generations. Results Novel breakthroughs in gene and protein interactions have been achieved in the field of male infertility using genome-wide proteomics and transcriptomics technologies. Conclusion Male Infertility is a complex and multifactorial disorder.Significance This review provides a comprehensive, up-todate evaluation of the multifactorial factors involved in male infertility. These factors need to be first assessed and understood before we can successfully treat male infertility.

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Section: Epigenetic Modifications and Male Infertilitymentioning

confidence: 99%

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Tahmasbpour

Balasubramanian

Agarwal

2014

J Assist Reprod Genet

109

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“…Expression of CaMKIV has been reported in rodent male germ cells (Wu and Means, 2000), though its presence, localization and role in sperm are still unknown. Anti-CaMKIV antibodies were used to determine whether this enzyme is present in human sperm by western blotting.…”

Section: Inhibition Of Cam Kinases Reduces Sperm Atp Contentmentioning

confidence: 99%

“…Regarding the testis, CaMKIV has been localized to mouse spermatogonia and spermatids. In elongating spermatids (transcriptionally inactive cells), CaMKIV is associated with the nuclear matrix and might be involved in chromatin remodelling during nuclear condensation (Wu and Means, 2000). Two recent studies focused on CaMKIV-deficient mice fertility: one reported that these animals are infertile owing to impaired spermiogenesis and replacement of basic nuclear proteins by protamines (Wu et al, 2000a), the other found that they exhibited normal spermatogenesis and fertility (Blaeser et al, 2001).…”

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confidence: 99%

Evidence of the presence of calcium/calmodulin-dependent protein kinase IV in human sperm and its involvement in motility regulation

Marı́n-Briggiler

Jha

Chertihin

et al. 2005

Journal of Cell Science

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The mechanisms involved in the regulation of mammalian sperm motility are not well understood. Calcium ions (Ca2+) have been suggested to play a key role in the maintenance of motility; nevertheless, how Ca2+ modulates this process has not yet been completely characterized. Ca2+ can bind to calmodulin and this complex regulates the activity of multiple enzymes, including Ca2+/calmodulin-dependent protein kinases (CaM kinases). Results from this study confirmed that the presence of Ca2+ in the incubation medium is essential for maintaining human sperm motility. The involvement of CaM kinases in Ca2+ regulation of human sperm motility was evaluated using specific inhibitors (KN62 and KN93) or their inactive analogues (KN04 and KN92 respectively). Sperm incubation in the presence of KN62 or KN93 led to a progressive decrease in the percentage of motile cells; in particular, incubation with KN62 also reduced sperm motility parameters. These inhibitors did not alter sperm viability, protein tyrosine phosphorylation or the follicular fluid-induced acrosome reaction; however, KN62 decreased the total amount of ATP in human sperm. Immunological studies showed that Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) is present and localizes to the human sperm flagellum. Moreover, CaMKIV activity increases during capacitation and is inhibited in the presence of KN62. This report is the first to demonstrate the presence of CaMKIV in mammalian sperm and suggests the involvement of this kinase in the regulation of human sperm motility.

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“…The resulting pellet containing the nuclear matrix fraction was solubilised in urea buffer (8 M urea, 0.1 M NaH2PO4, 0.01 M Tris, pH 8.) as described (Reyes et al, 1997;Wu and Means, 2000).…”

Section: Chromatin and Nuclear Matrix Preparationmentioning

confidence: 99%

“…To test this, we prepared nuclear matrix as reported (Wu and Means, 2000). In the first fractionation step, soluble proteins were extracted with Triton X-100.…”

Section: Copurification Of Kin17 With Poly(a + ) Rnamentioning

confidence: 99%

KIN17 encodes an RNA-binding protein and is expressed during mouse spermatogenesis

Pinon‐Lataillade

Masson

Bernardino-Sgherri

et al. 2004

Journal of Cell Science

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Genotoxic agents deform DNA structure thus eliciting a complex genetic response allowing recovery and cell survival. The Kin17 gene is up-regulated during this response. This gene encodes a conserved nuclear protein that shares a DNA-binding domain with the bacterial RecA protein. The KIN17 protein binds DNA and displays enhanced expression levels in proliferating cultured cells, suggesting a role in nuclear metabolism. We investigated this by studying the expression profile of KIN17 protein during mouse spermatogenesis. As expected, the expression level of Kin17 is higher in proliferating than in differentiated cells. KIN17 is selectively extracted from this tissue by detergents and a fraction was tightly associated with the nuclear matrix. Germinal cells ubiquitously express Kin17 and the protein is located mainly in the nucleus except in elongated spermatids where cytoplasmic staining is also observed. Sertoli and germ cells that are no longer mitotically active express KIN17, suggesting a general role in all testicular cell types. In adult testis a significant proportion of KIN17 co-purifies with polyadenylated RNA. KIN17 directly binds RNA, preferentially poly(G) and poly(U) homopolymers. These results together with the identification of KIN17 as a component of the human spliceosome indicate that this protein may participate in RNA processing.

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Ca2+/Calmodulin-dependent Protein Kinase IV Is Expressed in Spermatids and Targeted to Chromatin and the Nuclear Matrix

Cited by 59 publications

References 40 publications

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

Evidence of the presence of calcium/calmodulin-dependent protein kinase IV in human sperm and its involvement in motility regulation

KIN17 encodes an RNA-binding protein and is expressed during mouse spermatogenesis

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