Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress

Ma, Yan; Wang, Pei; Gu, Zhenxin; Tao, Yang; Shen, Changwei; Zhou, Yulin; Ye, Han; Yang, Runqiang

doi:10.1016/j.fochx.2019.100023

Cited by 35 publications

(20 citation statements)

References 38 publications

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“…Moreover, according to And and Wayne (2003), an elevated Ca 2+ concentration in cytosol and Ca 2+ binding with calmodulin (CaM) is a result of barley response to biotic stress, which explains the relationship between the crop low resistance and an elevated concentration of Ca in pots with Chlorella sp. It is also noteworthy that Ca takes part in signal transduction for GABA, which initiates phenol accumulation in barley which, in turn, justifies the BPA content elevated in the above-ground parts of barley, as found in this study (Ma et al 2019). It was found in a study conducted by Alobwede et al (2019) that Chlorella sp.…”

Section: The Effect Of Bpa On Spring Barleysupporting

confidence: 83%

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

2020

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Purpose The knowledge about the impact of BPA on soil health does not correspond to the great interest in its analogues. Therefore, a study was conducted to determine the potentially negative impact of BPA on the biochemical properties of soil. The study also evaluated the effectiveness of two biostimulants in eliminating potential homeostasis disorders caused by BPA. Materials and methods A pot experiment was conducted under supervised conditions. BPA at five contamination levels was added to the soil of the granulometric composition of sandy loam at 0, 0.1, 2, 40 and 800 mg BPA kg −1 of a dry matter (DM) of soil. The biochemical activity of the soil was interpreted through the activity of dehydrogenases (Deh), urease (Ure), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), arylsulphatase (Aryl) and β-glucosidase (Glu) whose activity was determined on days 5, 15 and 45 of the study. The biostimulative potential of Chlorella sp. and rhamnolipid 90 (which eliminates the undesirable effects of BPA on the parameters) was expressed with IF B-the factor of the impact of increasing of bisphenol (BP) soil contamination levels. The response of spring barley to increasing BPA pressure was analysed with the plant resistance index (RS). The study was made more comprehensive by determination of the macronutrient content in the plants. Results and discussion The sensitivity of individual enzymes to increasing bisphenol pressure on the 45th day of the experiment can be arranged in the following sequence: Deh > Ure > Glu > Pac > Cat > Aryl > Pal. Biostimulation of soil with Chlorella sp. gave better results than with rhamnolipid 90. A compilation of BPA 800 mg BPA kg −1 DM of soil and Chlorella sp. brought about an increase in the activity of Glu on the 45th day of the experiment and Pac, Pal and Aryl on the 5th day. Only at this contamination level did BPA stimulate the crop growth in all the parallel plots except in those biostimulated by Chlorella sp. Only algae significantly reduced the negative BPA impact on the N, Ca and K content in spring barley. Conclusions The experiment emphasised the significant inhibitory impact of BPA on the biochemical activity of soil which, in consequence, upset the microbial balance of soil processes. Chlorella sp. played a more important role in maintaining the soil homeostasis than rhamnolipid 90, which did not correspond to its negative impact on the yield of spring barley.

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Section: The Effect Of Bpa On Spring Barleysupporting

confidence: 83%

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

2020

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“…Under Cd stress, Cd 2+ can replace Ca 2+ binding site in OEC and inhibit OEC activity (Faller et al 2005). GABA can regulate intracellular Ca 2+ concentration and stress response related to Ca signal (Gut et al 2009), GABA treatment significantly increased the contents of calcium and calmodulin in barley (Gut et al 2009), and induced the influx of Ca 2+ in root tip cells of barley (Ma et al 2019). Therefore, the increase of Ca 2+ concentration in tobacco leaves induced by GABA may be one of the important reasons for the increase of OEC stability under Cd stress.…”

Section: Discussionmentioning

confidence: 99%

Exogenous γ-glutamic acid (GABA) induces proline and glutathione synthesis in alleviating Cd-induced photosynthetic inhibition and oxidative damage in tobacco leaves

Liu

Li³

et al. 2021

Journal of Plant Interactions

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The effects of exogenous γ-glutamic acid (GABA) on photosynthetic gas exchange capacity, PSII function, proline (Pro) synthesis and ROS metabolism of tobacco leaves under cadmium (Cd) stress were studied. The results showed that both stomatal and non-stomatal factors were the important factors to limit the photosynthetic capacity of tobacco leaves under Cd stress. Pro accumulation and glutathione (GSH) content increase are the important ways for tobacco leaves to adapt to Cd stress. Exogenous GABA significantly increased the stability of oxygen-evolving complex (OEC) and the electron transfer rate of PSII in tobacco leaves under Cd stress, and increased G s and P n in varying degrees. Exogenous GABA also increased the expression of P5CS and P5CR genes in tobacco leaves under Cd stress, thus promoting Pro accumulation. In conclusion, 0.5 mmol•L −1 exogenous GABA can not only improve the carbon assimilation ability by increasing the G s , but also alleviate the oxidative damage by promoting the synthesis and accumulation of Pro and GSH, and improve the stability of photosynthetic function of tobacco leaves under Cd stress.

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“…Lignin and related secondary metabolites not only act as physical barriers to invading plant pathogens but can also play a key role in plant defense signaling 23 . An increase in the lignin/phenylpropanoid content has been shown as a plant response to various abiotic stresses [24][25][26][27] . Therefore, an increase in the intensity of these molecules (1610-1630 cm −1 ) in HLB infected samples suggests that plants build up these molecules to combat the proliferation of bacteria within the tissues.…”

Section: Resultsmentioning

confidence: 99%

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

Sanchez

Pant

Mandadi

et al. 2020

Sci Rep

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Raman spectroscopy (RS) is an emerging analytical technique that can be used to develop and deploy precision agriculture. RS allows for confirmatory diagnostic of biotic and abiotic stresses on plants. Specifically, RS can be used for Huanglongbing (HLB) diagnostics on both orange and grapefruit trees, as well as detection and identification of various fungal and viral diseases. The questions that remain to be answered is how early can RS detect and identify the disease and whether RS is more sensitive than qPCR, the "golden standard" in pathogen diagnostics? Using RS and HLB as case study, we monitored healthy (qPCR-negative) in-field grown citrus trees and compared their spectra to the spectra collected from healthy orange and grapefruit trees grown in a greenhouse with restricted insect access and confirmed as HLB free by qPCR. Our result indicated that RS was capable of early prediction of HLB and that nearly all in-field qPCR-negative plants were infected by the disease. Using advanced multivariate statistical analysis, we also showed that qPCR-negative plants exhibited HLB-specific spectral characteristics that can be distinguished from unrelated nutrition deficit characteristics. These results demonstrate that RS is capable of much more sensitive diagnostics of HLB compared to qPCR. Plant diseases can be caused by a variety of different pathogens, such as viruses, fungi and bacteria. Huanglongbing (HLB), or citrus greening, is a devastating disease that plagues citrus trees in Asia, Africa, and more recently the Americas 1. In the United States, HLB is associated with Candidatus Liberibacter asiaticus (CLas), a gram-negative bacterium that inhabits the plant phloem in uneven and variable titers 2,3. Limited knowledge is available on CLas bacterium due to our inability to culture it in the laboratory. The bacterium is transmitted by the highly mobile Asian citrus psyllid (Diaphorina citri) enabling proliferation of HLB on large agricultural areas. The disease is also able to spread by grafting. In late stages, HLB-infected plants have asymmetric mottling on the leaves and lopsided and green fruits. HLB causes fruit drop and poor fruit quality, e.g. smaller size, less juice yield, higher acidity and lower sugar content. Early stages of the disease, however, have minimal to no symptoms and the bacteria can remain undetectable even by quantitative polymerase chain reaction (qPCR) in the trees for several years after initial infection 4. This, wherein, lies a significant challenge in regard to HLB management. Farmers are reluctant to remove trees that have normal, healthy appearances and keep producing fruits. However, if such trees have CLas bacteria, they allow for psyllids to quickly spread this devastating disease over large distances. Therefore, it becomes critical to detect HLB at the early (pre-symptomatic) stage and develop appropriate intervention strategies for HLB containment. In disease diagnostics, the most predominant methods used to confirm a pathogen are PCR and/or enzyme-linked immunosorbent as...

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Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress

Abstract: Graphical abstract

Cited by 35 publications

References 38 publications

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

Role of Chlorella sp. and rhamnolipid 90 in maintaining homeostasis in soil contaminated with bisphenol A

Exogenous γ-glutamic acid (GABA) induces proline and glutathione synthesis in alleviating Cd-induced photosynthetic inhibition and oxidative damage in tobacco leaves

Raman Spectroscopy vs Quantitative Polymerase Chain Reaction In Early Stage Huanglongbing Diagnostics

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