2022
DOI: 10.1038/s41467-022-33020-6
|View full text |Cite
|
Sign up to set email alerts
|

Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens

Abstract: Astrocytes are key elements of brain circuits that are involved in different aspects of the neuronal physiology relevant to brain functions. Although much effort is being made to understand how the biology of astrocytes affects brain circuits, astrocytic network heterogeneity and plasticity is still poorly defined. Here, we have combined structural and functional imaging of astrocyte activity recorded in mice using the Ca2+-modulated photoactivatable ratiometric integrator and specific optostimulation of gluta… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
13
0

Year Published

2023
2023
2024
2024

Publication Types

Select...
5
1
1

Relationship

2
5

Authors

Journals

citations
Cited by 15 publications
(14 citation statements)
references
References 73 publications
0
13
0
Order By: Relevance
“…However, how this specificity is modulated remains unknown. Furthermore, in a recently published study by our laboratory by Serra et al [3], the stimulation of three different glutamatergic nuclei resulted in differential astrocytic activity in the nucleus accumbens in a way that for each stimulated nucleus astrocytes not only responded in highly innervated areas, which supports the existence of different connected astrocyte subpopulations able to discern the input origin. This astrocytic discrimination between pathways was also previously observed by Perea and Araque [151].…”
Section: Definition Of Functional Astrocytic Ensemblesmentioning
confidence: 65%
See 3 more Smart Citations
“…However, how this specificity is modulated remains unknown. Furthermore, in a recently published study by our laboratory by Serra et al [3], the stimulation of three different glutamatergic nuclei resulted in differential astrocytic activity in the nucleus accumbens in a way that for each stimulated nucleus astrocytes not only responded in highly innervated areas, which supports the existence of different connected astrocyte subpopulations able to discern the input origin. This astrocytic discrimination between pathways was also previously observed by Perea and Araque [151].…”
Section: Definition Of Functional Astrocytic Ensemblesmentioning
confidence: 65%
“…Additionally, in an in vivo study carried out by Li et al [107], a combination of magnetic resonance imaging (MRI) with an AAV vector carrying a reporter was used to uncover dynamic Ca 2+ changes in astrocytes. Furthermore, in our laboratory, we have recently adapted a functional tool to monitor astrocyte activity called CaMPARI GFAP , which is a GECI for astrocytes that undergoes irreversible fluorescence conversion when there is elevated intracellular Ca 2+ and simultaneous delivery of 405 nm light [3]. Apart from allowing the study in a temporally controlled manner, it enables the labeling of a specific set of astrocytes that are active during a certain time window resulting in functional maps of the instantaneous picture of astrocyte activity.…”
Section: Available Methods For Tracking Astrocytesmentioning
confidence: 99%
See 2 more Smart Citations
“…Notably, unlike most GECIs, CaMPARI’s fluorescence becomes dimmer when intracellular Ca 2+ concentration increases. Previous works in rodents with CaMPARI used its PC capabilities to perform experiments like mapping of functional connectivity ( Zolnik et al, 2017 ), identifying neuron-astrocyte circuitry ( Serra et al, 2022 ), and recording of large-scale, volumetric neuronal activity from freely moving mice without any mechanical restriction ( Das et al, 2022 ). Recently, a new generation of CaMPARI, CaMPARI2, was developed with enhanced PC rate and brighter red fluorescence compared to the first CaMPARI generation (CaMPARI1).…”
Section: Introductionmentioning
confidence: 99%