Ca2+ signaling in plant manganese uptake: CPK21/23 kinases phosphorylate and activate manganese transporter NRAMP1

Huang, Chao‐Feng

doi:10.1007/s44154-022-00067-w

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…The induction of TaN-RAMP3 by Mn deficiency implies precise transcriptional regulation. In addition, recent studies have shown that NRAMP1 was phosphorylated by the protein kinases CPK21/23 and CIPK23 in Arabidopsis (Fu et al 2022;Zhang et al 2023a;Huang 2022), The 24th residue of TaNRAMP3 protein is also serine, which corresponds to the Ser22 of AtNRAMP1, indicating that TaNRAMP3 may also undergo phosphorylated. Therefore, exploring the transcription factors and protein kinases that regulate TaNRAMP3 will be one of our future research.…”

Section: Discussionmentioning

confidence: 98%

TaNRAMP3 is essential for manganese transport in Triticum aestivum

Wang,

Zhang,

Cao

et al. 2023

Stress Biology

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Manganese (Mn) is an essential trace element for almost all living organisms. In plants, Mn deficiency, which is occurs in calcareous soils or alkaline soils, severely limiting crop yields. However, the potential mechanism of Mn transport in Triticum aestivum is still obscure. Here, we found that TaNRAMP3, a member of the naturally resistant macrophage protein (NRAMP) family in Triticum aestivum, is located in the plasma membrane of protoplasts and functions as an influx transporter for Mn in yeast (Δsmf1). The expression of TaNRAMP3 was induced under Mn-deficiency conditions. Furthermore, TaNRAMP3-RNAi plants exhibited a sensitive phenotype, while transgenic plants overexpressing TaNRAMP3 showed a tolerant phenotype. In addition, TaNRAMP3 rescued the sensitive phenotype of Arabidopsis nramp1 mutant under Mn deficiency condition. In summary, our study reveals the key role of TaNRAMP3 in Mn transport in Triticum aestivum, allowing it to adapt to Mn-deficiency stress. These findings provide new insights for the cultivation of Mn-deficiency tolerant wheat varieties.

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Section: Discussionmentioning

confidence: 98%

TaNRAMP3 is essential for manganese transport in Triticum aestivum

Wang,

Zhang,

Cao

et al. 2023

Stress Biology

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“…As a crucial signal molecule in eukaryotes, Ca 2+ signals have a significant impact on plant growth and development as well as how plants adapt to biotic and abiotic stresses (Luan & Wang, 2021; Chong et al ., 2022; Gorgues et al ., 2022; Köster et al ., 2022; Zhang et al ., 2022). CPKs act as Ca 2+ sensors, not only detecting these signals but also transducing them into phosphorylation events that regulate downstream targets (Boudsocq & Sheen, 2013; Huang, 2022; Liang & Zhang, 2022; Z. Wang et al ., 2023). BOR1 is a crucial protein for the efficient translocation of B from the root to the xylem under B‐deficient conditions (Takano et al ., 2002).…”

Section: Discussionmentioning

confidence: 99%

CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter

Wang,

Zhang,

et al. 2024

New Phytologist

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Summary Boron (B) is crucial for plant growth and development. B deficiency can impair numerous physiological and metabolic processes, particularly in root development and pollen germination, seriously impeding crop growth and yield. However, the molecular mechanism underlying boron signal perception and signal transduction is rather limited. In this study, we discovered that CPK10, a calcium‐dependent protein kinase in the CPK family, has the strongest interaction with the boron transporter BOR1. Mutations in CPK10 led to growth and root development defects under B‐deficiency conditions, while constitutively active CPK10 enhanced plant tolerance to B deficiency. Furthermore, we found that CPK10 interacted with and phosphorylated BOR1 at the Ser689 residue. Through various biochemical analyses and complementation of B transport in yeast and plants, we revealed that Ser689 of BOR1 is important for its transport activity. In summary, these findings highlight the significance of the CPK10‐BOR1 signaling pathway in maintaining B homeostasis in plants and provide targets for the genetic improvement of crop tolerance to B‐deficiency stress.

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Calcium signaling in plant mineral nutrition: From uptake to transport

Wang,

Chen,

et al. 2023

Plant Communications

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Ca2+ signaling in plant manganese uptake: CPK21/23 kinases phosphorylate and activate manganese transporter NRAMP1

Cited by 4 publications

References 9 publications

TaNRAMP3 is essential for manganese transport in Triticum aestivum

TaNRAMP3 is essential for manganese transport in Triticum aestivum

CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter

Calcium signaling in plant mineral nutrition: From uptake to transport

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