Ca2+ Signals Generated by CatSper and Ca2+ Stores Regulate Different Behaviors in Human Sperm*

Alasmari, Wardah Abdullah; Costello, Sarah; Correia, Joao; Oxenham, Senga K.; Morris, Jennifer; Fernandes, Leonor; Ramalho‐Santos, João; Kirkman-Brown, Jackson; Michelangeli, Francesco; Publicover, Stephen J.; Barratt, Christopher L.R.

doi:10.1074/jbc.m112.439356

Cited by 134 publications

(157 citation statements)

References 78 publications

(121 reference statements)

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…Sperm cell motility is regulated by intracellular pH and Ca 2C signalling (Darszon et al 2011, Alasmari et al 2013. In most species, sperm cells acquire motility shortly before arrival in the cauda epididymis, immediately after their release from the epididymis or soon after ejaculation.…”

Section: Sperm Cell Capacitation and The Role Of Prostasomesmentioning

confidence: 99%

“…Ryanodine receptors are Ca 2C channels that release Ca 2C from such intracellular stores in response to cyclic adenosine dinucleotide phosphate ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) (Park et al 2011). Increase in intracellular Ca 2C via CatSper channel opening and release of stored Ca 2C at the sperm neck provides two separate mechanisms for regulating motility (Alasmari et al 2013). Interestingly, long-lasting Ca 2C responses require the presence of prostasomes, and it has therefore been hypothesised that some Ca 2C signalling tools are not initially carried by the sperm cells but have to be delivered to them by prostasomes (Park et al 2011).…”

Section: Sperm Cell Capacitation and The Role Of Prostasomesmentioning

confidence: 99%

See 1 more Smart Citation

Prostasomes: extracellular vesicles from the prostate

Aalberts¹,

Stout²,

Stoorvogel³

2014

Reproduction

173

150

View full text Add to dashboard Cite

The term 'prostasomes' is generally used to classify the extracellular vesicles (EVs) released into prostatic fluid by prostate epithelial cells. However, other epithelia within the male reproductive tract also release EVs that mix with 'true' prostasomes during semen emission or ejaculation. Prostasomes have been proposed to regulate the timing of sperm cell capacitation and induction of the acrosome reaction, as well as to stimulate sperm motility where all three are prerequisite processes for spermatozoa to attain fertilising capacity. Other proposed functions of prostasomes include interfering with the destruction of spermatozoa by immune cells within the female reproductive tract. On the other hand, it is unclear whether the distinct presumed functions are performed collectively by a single type of prostasome or by separate distinct sub-populations of EVs. Moreover, the exact molecular mechanisms through which prostasomes exert their functions have not been fully resolved. Besides their physiological functions, prostasomes produced by prostate tumour cells have been suggested to support prostate cancer spread development, and prostasomes in peripheral blood plasma may prove to be valuable biomarkers for prostate cancer.

show abstract

Section: Sperm Cell Capacitation and The Role Of Prostasomesmentioning

confidence: 99%

Section: Sperm Cell Capacitation and The Role Of Prostasomesmentioning

confidence: 99%

Prostasomes: extracellular vesicles from the prostate

Aalberts¹,

Stout²,

Stoorvogel³

2014

Reproduction

173

150

View full text Add to dashboard Cite

show abstract

“…Here, the male gametes pass a window of time, ranging from hours to days, waiting for the oocyte and interaction with the oviductal epithelial cells. In this context, the spermatozoa are exposed to different gradients of either activating (i.e., bicarbonate, pH, calcium concentration, progesterone, serum proteins) or inhibiting (i.e., endocannabinoids) factors, which influence their metabolic activity and reactivity (Alasmari et al, 2013;Barboni et al, 2011;Ijiri et al, 2012;Wertheimer et al, 2013). Here, the spermatozoa undergo the process of capacitation, acquiring the full ability to fertilize.…”

Section: Introductionmentioning

confidence: 99%

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Bernabò

Ordinelli

Agostino

et al. 2014

OMICS: A Journal of Integrative Biology

View full text Add to dashboard Cite

The rapid growth of published literature makes biomedical text mining increasingly invaluable for unpacking implicit knowledge hidden in unstructured text. We employed biomedical text mining and biological networks analyses to research the process of sperm egg recognition and binding (SERB). We selected from the literature the molecules expressed either on spermatozoa or on oocytes thought to be involved in SERB and, using an automated literature search software (Agilent Literature Search), we realized a network, SERBN, characterized by a hierarchical scale free and a small world topology. We used an integrated approach, either based on selection of hubs or by a cluster analysis, to discern the key molecules of SERB. We found that in most cases some of them are not directly situated on spermatozoa and oocyte, but are dispersed in oviductal fluid or embedded in exosomes present in the perivitelline space. To confirm and validate our results, we performed further analyses using STRING and Reactome FI software. Our findings underscore that the fertility is not a property of gametes in isolation, but rather depends on the functional integrity of the entire reproductive system. These observations collectively underscore the importance of integrative biology in exploring biological systems and in rethinking of fertility mechanisms in the light of this innovative approach.

show abstract

“…Collection of the samples and the research protocol were approved by the Research Center of Male Disease of the Third Xiangya Hospital. Spermatozoa were purified by direct swim-up in human tubal fluid medium (HTF, Millipore, Bedford, MA, USA) as described previously (Alasmari et al, 2013) and were adjusted to a final concentration of 80 million cells/mL. Tubal fluid medium is a capacitating medium containing 25 mM NaHCO 3 and 3% human serum albumin, which mimics the physiological environment in the female reproductive tract as described previously (Luo et al, 2015).…”

Section: Sample Collectionmentioning

confidence: 99%

“…In the penetration assay, 1% methylcellulose solution was used to mimic the viscous conditions in the female reproductive tract (Alasmari et al, 2013). Methylcellulose was dissolved in HTF medium and introduced into 7.5-cm long flattened capillary tubes (1.0-mm inner depth; Elite Medical Co. Ltd., Nanjing, China) with one end sealed using plasticine.…”

Section: Penetration Of Artificial Viscous Mediummentioning

confidence: 99%

Toxic effects of 2,4-dichlorophenoxyacetic acid on human sperm function <i>in vitro </i>

et al. 2016

View full text Add to dashboard Cite

-The herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D) is globally used in agriculture and has been linked to human sperm abnormalities in vivo. However, its effects on ejaculated human spermatozoa in vitro have not been characterized. Therefore, we examined the effects of 2,4-D on the functions of ejaculated human spermatozoa in vitro, including: sperm motility, the ability to move through a viscous medium, capacitation, and the acrosome reaction. Different doses of 2,4-D (10 nM, 100 nM, 1 μM, 10 μM, 100 μM, and 200 μM) were applied to human spermatozoa prepared from normal fresh semen samples. The results indicated that 2,4-D did not affect the viability, capacitation, or spontaneous acrosome reactions of human spermatozoa, but it dose-dependently inhibited the total motility, progressive motility, ability to penetrate viscous medium, and progesterone-induced capacitation and acrosome reaction rates. These results suggest that exposure to 2,4-D and its accumulation in the seminal plasma and follicular fluid might increase the risk of infertility. Our findings provide new insights for understanding the male reproductive toxicity of 2,4-D.

show abstract

Ca2+ Signals Generated by CatSper and Ca2+ Stores Regulate Different Behaviors in Human Sperm*

Cited by 134 publications

References 78 publications

Prostasomes: extracellular vesicles from the prostate

Prostasomes: extracellular vesicles from the prostate

Network Analyses of Sperm–Egg Recognition and Binding: Ready to Rethink Fertility Mechanisms?

Toxic effects of 2,4-dichlorophenoxyacetic acid on human sperm function <i>in vitro </i>

Contact Info

Product

Resources

About