2020
DOI: 10.1128/mbio.01492-20
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CaaX-Like Protease of Cyanobacterial Origin Is Required for Complex Plastid Biogenesis in Malaria Parasites

Abstract: Plasmodium parasites and related apicomplexans contain an essential “complex plastid” organelle of secondary endosymbiotic origin, the apicoplast. Biogenesis of this complex plastid poses a unique challenge requiring evolution of new cellular machinery. We previously conducted a mutagenesis screen for essential apicoplast biogenesis genes to discover organellar pathways with evolutionary and biomedical significance. Here we validate and characterize a gene candidate from our screen, Pf3D7_0913500. Using a cond… Show more

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Cited by 5 publications
(2 citation statements)
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“…Previous studies suggest that the primary role of the apicoplast during the ABS is to synthesize isoprenoids 28 . Indeed, genetic knockdown of many essential apicoplast genes can be rescued by exogenous isopentenyl pyrophosphate (IPP, 200 µM) 24,[50][51][52][53][54][55][56][57][58][59][60] . We thus attempted to rescue PfDyn2-deficient parasites with IPP.…”
Section: Pfdyn2 Is Essential For Asexual Stage Developmentmentioning
confidence: 99%
“…Previous studies suggest that the primary role of the apicoplast during the ABS is to synthesize isoprenoids 28 . Indeed, genetic knockdown of many essential apicoplast genes can be rescued by exogenous isopentenyl pyrophosphate (IPP, 200 µM) 24,[50][51][52][53][54][55][56][57][58][59][60] . We thus attempted to rescue PfDyn2-deficient parasites with IPP.…”
Section: Pfdyn2 Is Essential For Asexual Stage Developmentmentioning
confidence: 99%
“…fatty acid biosynthesis inhibitors) that are apparently off‐target and have no impact on the apicoplast (Uddin et al, 2018 ; Wu et al, 2015 ). IPP rescue has also turned out to be a convenient tool to confirm the indispensability of blood stage apicoplast‐targeted genes, pathways, and membrane transporters where knockouts would otherwise have been lethal (Meister et al, 2020 ; Sayers et al, 2017 ; Tan et al, 2021 ; Tang et al, 2019 ; Walczak et al, 2018 ). IPP supplementation has also delivered the answer to one of the most enduring mysteries since the discovery of the apicoplast, namely why do malaria parasites take two blood stage cycles to die when their apicoplasts are compromised?…”
Section: The Apicoplast During Blood Stages—some Updatesmentioning
confidence: 99%