Cabozantinib promotes erythroid differentiation in K562 erythroleukemia cells through global changes in gene expression and JNK activation

Fu, Yu-Hsuan; Ou, Da-Liang; Yang, Yanou; Su, Kuan-Wei; Chen, Chien Yuan; Tien, Hwei‐Fang; Lai, Zheng Sheng; Shen, Che Kun James; Chien, Hsiung–Fei; Lin, Liang-In

doi:10.1038/s41417-021-00358-w

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…RNAi knockdown of SRPK1 inhibited the proliferation and induced apoptosis of K562 cells [ 35 ]. Cabozantinib induced erythroid differentiation of K562 cells by upregulating heme biosynthesis, globin synthesis and erythrocyte-related reactions [ 36 ]. Ormeloxifene led to increased levels of markers of megakaryocyte differentiation CD41, and CD61 as well as key transcription factors GATA1 and AML1 in K562 cells [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway

Zhao,

Che,

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway

Zhao,

Che,

et al. 2024

Heliyon

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“…Second, the erythroid-induced differentiation of the KO clones in this experiment was not sufficient. Further optimization may be necessary, either by optimizing experimental conditions or by using other induction reagents such as cabozantinib [ 28 ]. Lastly, it is important to note that the K562 cell line, being an erythroleukemia cell line, has a slightly different natural expression of blood group antigens compared to erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene

Yang,

Li,

et al. 2023

Transfus Med Hemother

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Introduction: The Vel– phenotype is a rare blood group, and it is challenging for identifying this phenotype due to limited available reagents. Moreover, there are relatively few studies on genomic editing of erythroid antigens and generation of knockout (KO) cell lines at present. Methods: To identify the high-efficiency small-guiding RNA (sgRNA) sequence, candidate sgRNAs were transfected into HEK 293T cells and analyzed using Sanger sequencing. Following this, the high-efficiency sgRNA was transfected into K562 cells using lentivirus transduction to generate KO Vel blood group gene cells. The expression of the Vel protein was detected using Western blot on single-cell clones. Additionally, flow cytometry was used to detect the erythroid markers CD235a and CD71. Hemoglobin quantification and Giemsa staining were also performed to evaluate the erythroid differentiation of KO clones induced by hemin. Results: The high-efficiency sgRNA was successfully obtained and used for CRISPR-Cas9 editing in K562 cells. After limiting dilution and screening, two KO clones had either deleted 2 or 4 bases and showed no expression of the Vel protein. In the hemin-induced KO clone, there was a significant difference in erythroid marker and hemoglobin quantification compared to untreated cells. The morphological changes were also observed for the hemin-induced KO clone. Conclusion: In this study, a highly efficient sgRNA was screened out and used to generate Vel erythroid antigen KO single-cell clones in K562 cells. The edited cells could then be induced to undergo erythroid differentiation with the use of hemin.

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The unexpected impact of cabozantinib on red blood cells consumption in patients with transfusion-dependent thalassemia

et al. 2022

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Cabozantinib promotes erythroid differentiation in K562 erythroleukemia cells through global changes in gene expression and JNK activation

Cited by 6 publications

References 48 publications

Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway

Asperuloside regulates the proliferation, apoptosis, and differentiation of chronic myeloid leukemia cell line K562 through the RAS/MEK/ERK pathway

CRISPR/Cas9-Editing K562 Cell Line as a Potential Tool in Transfusion Applications: Knockout of Vel Antigen Gene

The unexpected impact of cabozantinib on red blood cells consumption in patients with transfusion-dependent thalassemia

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