Cactin, a conserved protein that interacts with the Drosophila IκB protein Cactus and modulates its function

Lin, Pei; Huang, Leslie H.; Steward, Ruth

doi:10.1016/s0925-4773(00)00314-2

Cited by 36 publications

(61 citation statements)

References 48 publications

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“…In addition over-expression of I-jB kinase 1 (IKK 1), an inhibitor of zebrafish NF-jB, generates the same phenotype as observed in mIjBa morphants (Correa et al, 2005), suggesting that blocking NF-jB affects caudal mesoderm development in zebrafish. Such a developmental role for NF-jB in conjunction with our studies demonstrating that knockdown of zCactin manifests a shortened tail phenotype is consistent with the positive effect of Cactin on Dorsal signalling in Drosophila (Lin et al, 2000).…”

supporting

confidence: 88%

“…One such molecule is Cactin. The latter was initially identified in Drosophila as a novel Cactus-interacting protein by using Cactus as bait in a yeast-two hybrid screen (Lin et al, 2000). Drosophila Cactin was shown to be maternally inherited and its over-expression furthered the Cactus mutant phenotype in Drosophila by enhancing embryonic lethality and ventralisation (Lin et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

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Characterisation of expression patterns and functional role of Cactin in early zebrafish development

Atzei

Yang

Collery

et al. 2010

Gene Expression Patterns

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a b s t r a c tThe immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-jB that plays dualist roles in innate immunity and early development. Indeed NF-jB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-jB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal-ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organisation. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.

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supporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Characterisation of expression patterns and functional role of Cactin in early zebrafish development

Atzei

Yang

Collery

et al. 2010

Gene Expression Patterns

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“…Cactin was identified as a novel protein interacting with Cactus, the Drosophila ortholog of IκBα, which is important for negative regulation of the NFκB signaling pathway [28]. It has been reported that [31][32][33]28].…”

Section: Discussionmentioning

confidence: 99%

“…Given that Drosophila Cactin interacts with Cactus, which is known as an insect ortholog of mammalian IκBα [28], and that IκBα is an important negative regulator for NFκB [29], we hypothesized that Cactin may be one of the downstream genes of NFκB and may be induced by activation of NFκB. To examine whether Cactin is induced by activation of NFκB, we investigated the mRNA expression levels of Cactin after TNFα stimulation.…”

Section: Cactin Provides a Negative Feedback Loop In The Nfκb Pathwaymentioning

confidence: 99%

TRIM39 negatively regulates the NFκB-mediated signaling pathway through stabilization of Cactin

Suzuki

Watanabe

Nakamaru

et al. 2015

Cell. Mol. Life Sci.

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NFκB is one of the central regulators of cell survival, immunity, inflammation, carcinogenesis and organogenesis. The activation of NFκB is strictly regulated by several posttranslational modifications including phosphorylation, neddylation and ubiquitination. Several types of ubiquitination play important roles in multi-step regulations of the NFκB pathway. Some of the tripartite motif-containing (TRIM) proteins functioning as E3 ubiquitin ligases are known to regulate various biological processes such as inflammatory signaling pathways. One of the TRIM family proteins, TRIM39, for which the gene has single nucleotide polymorphisms (SNPs), has been identified as one of the genetic factors in Behcet's disease. However, the role of TRIM39 in inflammatory signaling had not been fully elucidated. In this study, to elucidate the function of TRIM39 in inflammatory signaling, we performed yeast two-hybrid screening using TRIM39 as a bait and identified Cactin, which has been reported to inhibit NFκB and TLR-mediated transcriptions. We show that TRIM39 stabilizes Cactin protein and that Cactin is upregulated after TNFα stimulation. TRIM39 knockdown also causes activation of the NFκB signal.These findings suggest that TRIM39 negatively regulates the NFκB signal in collaboration with Cactin induced by inflammatory stimulants such as TNFα.

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“…Ψ는 소수성아미노산, X는 임의의 아미노산)에서 나타난다 (Karin, 1999;Shirane et al, 1999). 면역신호에 따라 이 serine 부 위가 인산화되면, ubiquitin 단백질에 의해 인식되고, 이를 단백 질 분해 효소에 의해 IĸB가 분해되고, 결과적으로 NFĸB가 IĸB 와의 결합에서 방출되게 된다 (Burke et al, 1999;Latimer et al, 1998;Shirane et al, 1999;Lin et al, 2000;Senftleben et al, 2001). 이와 유사하게 C 말단 부위도 인산화되어 단백질 분해효 소에 노출되게 된다 (Beg and Baldwin, 1993;Liu et al, 1997).…”

Section: 한국의 전 지역에서 이러한 저항성 개체들을 분석한 결과 L1014funclassified

An Integrated Biological Control Using an Endoparasitoid Wasp (Cotesia plutellae) and a Microbial Insecticide (Bacillus thuringiensis) against the Diamondback Moth, Plutella xylostella

Kim¹,

Kim²,

Park³

et al. 2013

Korean journal of applied entomology

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All tested Korean populations of the diamondback moth, Plutella xylostella, are known to be resistant especially against pyrethroid insecticides by mutation in its molecular target, para-sodium channel. Moreover, P. xylostella is able to develop resistance against most commercial insecticides. This study was performed to develop an efficient control technique against P. xylostella by a combined treatment of an endoparasitoid wasp, Cotesia plutellae, and a microbial insecticide, Bacillus thurigiensis. To investigate any parasitism preference of C. plutellae against susceptible and resistant P. xylostella, five different populations of P. xylostella were compared in insecticide susceptibilities and parasitism by C. plutellae. These five P. xylostella populations showed a significant variation against three commercial insecticides including pyrethroid, organophosphate, neonicotinoid, and insect growth regulator. However, there were no significant differences among five P. xylostella populations in their parasitic rates by C. plutellae. Moreover, parasitized larvae of P. xylostella showed significantly higher susceptibility to B. thuringiensis. As an immunosuppressive agent, viral ankyrin genes (vankyrins) encoded in C. plutellae were transiently expressed in nonparasitized larvae. Expression of vankyrins significantly enhanced the efficacy of B. thuringiensis against the third instar larvae of P. xylostella. Thus an immunosuppression induced by C. plutellae enhanced the insecticidal efficacy of B. thuringiensis. These results suggest that a combined treatment of C. plutellae and B. thuringiensis may effectively control the insecticide-resistant populations of P. xylostella.

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Cactin, a conserved protein that interacts with the Drosophila IκB protein Cactus and modulates its function

Cited by 36 publications

References 48 publications

Characterisation of expression patterns and functional role of Cactin in early zebrafish development

Characterisation of expression patterns and functional role of Cactin in early zebrafish development

TRIM39 negatively regulates the NFκB-mediated signaling pathway through stabilization of Cactin

An Integrated Biological Control Using an Endoparasitoid Wasp (Cotesia plutellae) and a Microbial Insecticide (Bacillus thuringiensis) against the Diamondback Moth, Plutella xylostella

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