This study evaluated the effects of N/P fertilization on the chemical, mineral composition, and in vitro degradation kinetics of cactus pear cv. Gigante. The research was conducted in Quixadá and Tejuçuoca, CE, Brazil. Nine N/P combinations were evaluated from five levels of N (10, 70, 100, 130, and 190 kg ha −1 year −1 ), five levels of P 2 O 5 (10, 70, 100, 130, and 190 kg ha −1 year −1 ), and two harvest frequencies (annual and biannual). The experiment was performed in a split plot completely randomized block design, with four replications. The maximum CP content was obtained in biannual harvest in Quixadá and Tejuçuoca with the combined levels N/P 2 O 5 of 190/190 and 130.03/190 kg ha −1 year −1 , respectively. Calcium content was reduced by fertilization, which allowed to reduce the Ca:P ratio to 9.72:1.00 with the N/P 2 O 5 combination at 190/10 kg ha −1 year −1 in the biannual harvest. The increase in N and P levels results in higher S and Mn content and higher digestibility. The annual harvest improves quality in Quixadá. However, it has weaker influence on quality in Tejuçuoca. In Tejuçuoca, Mg, Cu, and Mn contents are lower under annual harvest. Sulfur content is lower under annual harvest in both sites.