CAD/CAM scaffolds for bone tissue engineering: investigation of biocompatibility of selective laser melted lightweight titanium

Naujokat, Hendrik; Rohwedder, Johanna; Gülses, Aydın; Aktas, Oral Cenk; Wiltfang, Jens; Açil, Yahya

doi:10.1049/iet-nbt.2019.0320

Cited by 5 publications

(4 citation statements)

References 53 publications

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“…(D640/20) and was conducted according to the guidelines of the Helsinki Declaration of Human Rights. Osteoblast cultivation was conducted according to the technique described by Naujokat et al [ 23 ] Human osteoblasts were obtained from patients who had undergone bone graft harvesting procedures of cancellous bone from the crista iliaca anterior at the Oral and Maxillofacial Surgery Department at the Christian Albrechts University Hospital Schleswig-Holstein, Kiel Campus, Kiel, Germany. The osseous samples were transferred into the 89% Dulbecco’s Modified Eagle’s Minimum Essential Medium (DMEM) (PAA Laboratories GmbH, Pasching, Austria), 10% fetal calf serum (FCS) (Biochrom, Berlin, Germany, 1% Penicillin/Streptomycin, Biochrom, Berlin, Germany).…”

Section: Methodsmentioning

confidence: 99%

Initiated Chemical Vapor Deposition (iCVD) Functionalized Polylactic Acid–Marine Algae Composite Patch for Bone Tissue Engineering

et al. 2021

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The current study aimed to describe the fabrication of a composite patch by incorporating marine algae powders (MAPs) into poly-lactic acid (PLA) for bone tissue engineering. The prepared composite patch was functionalized with the co-polymer, poly (2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) (p(HEMA-co-EGDMA)) via initiated chemical vapor deposition (iCVD) to improve its wettability and overall biocompatibility. The iCVD functionalized MAP–PLA composite patch showed superior cell interaction of human osteoblasts. Following the surface functionalization by p(HEMA-co-EGDMA) via the iCVD technique, a highly hydrophilic patch was achieved without tailoring any morphological and structural properties. Moreover, the iCVD modified composite patch exhibited ideal cell adhesion for human osteoblasts, thus making the proposed patch suitable for potential biomedical applications including bone tissue engineering, especially in the fields of dentistry and orthopedy.

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Section: Methodsmentioning

confidence: 99%

Initiated Chemical Vapor Deposition (iCVD) Functionalized Polylactic Acid–Marine Algae Composite Patch for Bone Tissue Engineering

et al. 2021

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“…F1 (InterOss) and F3 (InterCollagen guiade) xenografts presented cell viability average values above 75%, demonstrating a low cytotoxic behavior against HeLa line cells. Different tests on xenografts have shown that these materials are biocompatible, with variable results in cell proliferation compared with the control group or other allogeneic or alloplastic materials [11,[50][51][52][53]. The biocompatibility observed for xenografts is consistent with the results obtained for F1 (InterOss) and F3 (InterCollagen Guide), which show very low percentages of cytotoxicity, in contrast to F2 (InterOss Collagen), which present a critical portion of apparent cytotoxic results when compared with the negative control.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

2022

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Bone substitutes based on xenografts have been used for a long time in bone regeneration thanks to their inductive capacity for bone tissue regeneration. Some bone-based scaffolds have been modified by adding collagen and other proteins to improve their regenerative capacity and prevent migration and aggregation, especially particles. However, rejection of this graft has been reported due to protein residues caused by poor material preparation. We compared the in vitro and in vivo biological response of two commercial xenografts (InterOss®, F1 and InterOss® Collagen, F2) and a commercial porcine collagen membrane (InterCollagen® Guide, F3) as a rapid degradation control. Fourier Transform Infrared Spectroscopy (FT-IR) analysis evidenced the presence of hydroxyl, orthophosphate, and carbonate groups of the xenografts and amide groups of collagen. Thermogravimetric analysis (TGA) of the xenografts demonstrated their thermal stability and the presence of a few amounts of organic material. The study by differential scanning calorimetry showed the presence of endothermic peaks typical of the dehydration of the xenografts (F1 and F2) and for the collagen membrane (F3), the beginning of structural three-dimensional protein changes. Subsequently, in vitro biocompatibility tests were carried out for the materials with Artemia salina and MTT cell viability with HeLa cells, demonstrating the high biocompatibility of the materials. Finally, in vivo biocompatibility was studied by implanting xenografts in biomodels (Wistar rats) at different periods (30, 60, and 90 days). The F1 xenograft (InterOss) remained remarkably stable throughout the experiment (90 days). F2 (InterOss Collagen) presented a separation of its apatite and collagen components at 60 days and advanced resorption at 90 days of implantation. Finally, the collagen membrane (F3) presented faster resorption since, at 90 days, only some tiny fragments of the material were evident. All the in vivo and in vitro test results demonstrated the biocompatibility of the xenografts, demonstrating the potential of these materials for tissue engineering.

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“…Critical-sized bone defects resulting from trauma, inflammation, or tumor resection ( Wang et al, 2020 ) are not able to heal without surgical intervention ( Maruyama et al, 2020 ; Naujokat et al, 2020 ). Often, these defects require the use of bone grafts, providing mechanical stability and support for the bone repair process.…”

Section: Introductionmentioning

confidence: 99%

Extrusion-based 3D printing of osteoinductive scaffolds with a spongiosa-inspired structure

Kühl,

Gorb,

Kern

et al. 2023

Front. Bioeng. Biotechnol.

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Critical-sized bone defects resulting from trauma, inflammation, and tumor resections are individual in their size and shape. Implants for the treatment of such defects have to consider biomechanical and biomedical factors, as well as the individual conditions within the implantation site. In this context, 3D printing technologies offer new possibilities to design and produce patient-specific implants reflecting the outer shape and internal structure of the replaced bone tissue. The selection or modification of materials used in 3D printing enables the adaption of the implant, by enhancing the osteoinductive or biomechanical properties. In this study, scaffolds with bone spongiosa-inspired structure for extrusion-based 3D printing were generated. The computer aided design process resulted in an up scaled and simplified version of the bone spongiosa. To enhance the osteoinductive properties of the 3D printed construct, polycaprolactone (PCL) was combined with 20% (wt) calcium phosphate nano powder (CaP). The implants were designed in form of a ring structure and revealed an irregular and interconnected porous structure with a calculated porosity of 35.2% and a compression strength within the range of the natural cancellous bone. The implants were assessed in terms of biocompatibility and osteoinductivity using the osteosarcoma cell line MG63 and patient-derived mesenchymal stem cells in selected experiments. Cell growth and differentiation over 14 days were monitored using confocal laser scanning microscopy, scanning electron microscopy, deoxyribonucleic acid (DNA) quantification, gene expression analysis, and quantitative assessment of calcification. MG63 cells and human mesenchymal stem cells (hMSC) adhered to the printed implants and revealed a typical elongated morphology as indicated by microscopy. Using DNA quantification, no differences for PCL or PCL-CaP in the initial adhesion of MG63 cells were observed, while the PCL-based scaffolds favored cell proliferation in the early phases of culture up to 7 days. In contrast, on PCL-CaP, cell proliferation for MG63 cells was not evident, while data from PCR and the levels of calcification, or alkaline phosphatase activity, indicated osteogenic differentiation within the PCL-CaP constructs over time. For hMSC, the highest levels in the total calcium content were observed for the PCL-CaP constructs, thus underlining the osteoinductive properties.

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CAD/CAM scaffolds for bone tissue engineering: investigation of biocompatibility of selective laser melted lightweight titanium

Cited by 5 publications

References 53 publications

Initiated Chemical Vapor Deposition (iCVD) Functionalized Polylactic Acid–Marine Algae Composite Patch for Bone Tissue Engineering

Initiated Chemical Vapor Deposition (iCVD) Functionalized Polylactic Acid–Marine Algae Composite Patch for Bone Tissue Engineering

Biocompatibility Assessment of Two Commercial Bone Xenografts by In Vitro and In Vivo Methods

Extrusion-based 3D printing of osteoinductive scaffolds with a spongiosa-inspired structure

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