Cadaveric blood cards: Assessing DNA quality and quantity and the utility of STRs for the individual estimation of trihybrid ancestry and admixture proportions

West, Frankie; Algee‐Hewitt, Bridget F. B.

doi:10.1016/j.fsisyn.2020.03.002

Cited by 2 publications

(2 citation statements)

References 44 publications

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“…In spite of the limitations addressed below, such as unreliability of Penta D and Penta E genotypes involving specific alleles, this NGS-based STR database presents reliable allele frequencies that could be used in criminal casework to estimate the rarity of a given STR-based profile from a query sample of unknown or uncertain ancestry in various worldwide populations. This could instantly, and without additional costs, trigger a DNA-based intelligence strategy to guide enquiries [ 28 ] providing hints and/or assigning biogeographical origin in many situations, such as a missing person investigation [ 28 , 29 ], leaving only the most complex cases for supplementary analysis with a most suitable set of Ancestry Informative Markers.…”

Section: Discussionmentioning

confidence: 99%

Open-Access Worldwide Population STR Database Constructed Using High-Coverage Massively Parallel Sequencing Data Obtained from the 1000 Genomes Project

Frontanilla

Valle-Silva

Ayala

et al. 2022

Genes

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Achieving accurate STR genotyping by using next-generation sequencing data has been challenging. To provide the forensic genetics community with a reliable open-access STR database, we conducted a comprehensive genotyping analysis of a set of STRs of broad forensic interest obtained from 1000 Genome populations. We analyzed 22 STR markers using files of the high-coverage dataset of Phase 3 of the 1000 Genomes Project. We used HipSTR to call genotypes from 2504 samples obtained from 26 populations. We were not able to detect the D21S11 marker. The Hardy-Weinberg equilibrium analysis coupled with a comprehensive analysis of allele frequencies revealed that HipSTR was not able to identify longer alleles, which resulted in heterozygote deficiency. Nevertheless, AMOVA, a clustering analysis that uses STRUCTURE, and a Principal Coordinates Analysis showed a clear-cut separation between the four major ancestries sampled by the 1000 Genomes Consortium. Except for larger Penta D and Penta E alleles, and two very small Penta D alleles (2.2 and 3.2) usually observed in African populations, our analyses revealed that allele frequencies and genotypes offered as an open-access database are consistent and reliable.

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Section: Discussionmentioning

confidence: 99%

Open-Access Worldwide Population STR Database Constructed Using High-Coverage Massively Parallel Sequencing Data Obtained from the 1000 Genomes Project

Frontanilla

Valle-Silva

Ayala

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“…Hence, in this study, we collected samples from both Vietnamese Bouyei groups in Lao Cai (Tu Di) and Ha Giang (Bo Y) to investigate their population genetics using autosomal and Y chromosome short tandem repeats (STRs). While the autosomal STRs are suitable and highly informative markers to examine the genetic population structure and for individual identification purposes (Algee‐Hewitt et al, 2016; West & Algee‐Hewitt, 2020), the paternally inherited Y‐STRs have high‐mutation rates and lack of recombination, making them an ideal tool for inferring the recent population history (Koganebuchi et al, 2012; Xu et al, 2015). Our analysis will not only be valuable for forensic purposes, but also possibly reveal the debated origins and separation of the Bouyei peoples in Vietnam.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure and population connection of two Bouyei populations in northern Vietnam based on short tandem repeat analysis

Tran

Chu²,

Nguyen

et al. 2021

American J Hum Biol

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Objectives: Genetic characteristics were investigated based on short tandem repeat (STR) data to assess the relationship between two Vietnamese Bouyei populations in Vietnam. Methods: We collected hair and buccal swab samples from two separate Bouyei populations in the mountainous region of Northern Vietnam, which are the Bo Y in Ha Giang Province and the Tu Di in Lao Cai Province. The study included data of 23 autosomal and 27 Y-chromosome STRs loci of 96 unrelated participants from a total Vietnamese Bouyei population of under 3300 individuals. Results:The results showed that these STR markers are valuable for differentiation of individuals and human genetic studies in Vietnamese Bouyei populations. Genetic analysis indicated that Tu Di and Bo Y people were from the same Bouyei population in China. Conclusions:The results supported the official historical records of the region and the classification of the Vietnamese government. Furthermore, the genetic data provided in this study will be helpful in investigating the genetic genealogy evolution and settlement or migration patterns of the Bouyei populations in Vietnam.

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Cadaveric blood cards: Assessing DNA quality and quantity and the utility of STRs for the individual estimation of trihybrid ancestry and admixture proportions

Cited by 2 publications

References 44 publications

Open-Access Worldwide Population STR Database Constructed Using High-Coverage Massively Parallel Sequencing Data Obtained from the 1000 Genomes Project

Open-Access Worldwide Population STR Database Constructed Using High-Coverage Massively Parallel Sequencing Data Obtained from the 1000 Genomes Project

Genetic structure and population connection of two Bouyei populations in northern Vietnam based on short tandem repeat analysis

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