Cadaveric bone marrow as potential source of hematopoietic stem cells for transplantation

Abstract

Every year, bone marrow transplantation saves many lives worldwide. Unfortunately, a suitable donor is not always available. Since organs are routinely harvested from cadaveric organ donors, we decided to assess such a possibility for bone marrow. We analyzed the functional properties and phenotypic markers of murine hematopoietic stem and progenitor cells (HSPC) from cadaveric bone marrow and during storage in vitro in a suspension. It was demonstrated that HSPC exposed to a warm or cold ischemia in intact femur did not lose their phenotype and maintained their repopulating ability for two to twelve hours depending on the temperature. Additionally, fresh bone marrow remained fully viable in cell suspension for two days or four days at 37°C or 4°C, respectively. Based on these findings, cadaveric bone marrow could be considered as an alternative source of hematopoietic stem cells for transplantation.

Human cadaver multipotent stromal/stem cells isolated from arteries stored in liquid nitrogen for 5 years

“…These quiescent or dormancy stem cells survive by adapting to low oxygen consumption with a slow metabolism and no transcription program [23]. The isolation of viable and functional cadaveric stem cells from human bone marrow [24,25], brain [10,26] and muscle [12] up to many days postmortem is possible today, but cadaveric cryopreserved vessels remain unstudied.…”

Cadaveric cardiosphere-derived cells can maintain regenerative capacity and improve the heart function of cardiomyopathy

“…Some studies suggested that human pluripotent haematopoietic stem cells could survive in cadaveric bone marrow(BM). [17][18][19] It has also been shown previously that split-thickness skin grafts could be allografted up to 3 weeks after death if stored at 4 C. 20 More recently, stem and adult cells have been isolated from post mortem brain, spinal cord, and retinal tissue. 3,[21][22][23] These findings showed that, due to the persistence of stem cells, cadaver tissue could be a potential abundant source of these cells.…”

Salvage of Cadaver Stem Cells (CSCs) as a Routine Procedure: History or Future for Regenerative Medicine

“…When quality, as measured by colonyforming units, of CD marrow is compared with bone marrow aspirated from LD, they saw a tendency towards lower amounts in the cadaveric marrow, but the difference was not significant. As regards of costs, the method for harvesting and procurement of cadaveric bone marrow was relatively simple and fast, when used together with organ retrieval [11,12]. In 1986 a 12 year old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction.…”

“…Recent studies have shown that CBM cells could be stored up to seven days without an increase in apoptosis and that three days of storage does not affect the CD34+ fraction of the cells. The bone marrow could be harvested in case of a sudden death, phenotyped and transplanted immediately or stored for later use [12]. Neurosphere-generating cells can be isolated from adult mouse spinal cord and forebrain subependymal zone after postmortem intervals of up to 140 h, when kept at 4°C, and up to 30 h when kept at room temperature.…”

“…Organ and tissue from this origin is a widely utilized and an obvious strategy for transplantation to treat heart, kidney, lung, liver, eye, bone, skin disease, among others [3][4][5][6][7][8][9]. But there is a reasonable possibility also to isolate in the near future, stem cells from these same cadaveric organs and tissues that could give new therapeutics options for many patients with severe lesions and diseases [10][11][12]. The number of hematopoietic stem cells (HSC) transplantations utilizing marrow, peripheral and cord blood cells has increased these last years also the demand on a wider access to the different sources of human HSC.…”