Cadaverine in the brain of axenic mice

Stepita-Klauco, Matej; Doležalová, Hana

doi:10.1038/252158a0

Cited by 27 publications

(6 citation statements)

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“…Interestingly, it is not only the activity of ODC that increases; arginine decarboxylase activity increases too. Regarding cadaverine, there is an endogenous source of this diamine in the mouse and it is actually synthesized in mouse brain (Stepitak and Dolezalo 1974 ). In addition, both putrescine and cadaverine are substrates of various amine oxidases (Schmidt-Glenewinkel et al 1977 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

et al. 2017

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Understanding the human brain is the ultimate goal in neuroscience, but this is extremely challenging in part due to the fact that brain tissue obtained from autopsy is practically the only source of normal brain tissue and also since changes at different levels of biological organization (genetic, molecular, biochemical, anatomical) occur after death due to multiple mechanisms. Here we used metabolomic and anatomical techniques to study the possible relationship between post-mortem time (PT)-induced changes that may occur at both the metabolomics and anatomical levels in the same brains. Our experiments have mainly focused on the hippocampus of the mouse. We found significant metabolomic changes at 2 h PT, whereas the integrity of neurons and glia, at the anatomical/ neurochemical level, was not significantly altered during the first 5 h PT for the majority of histological markers.Electronic supplementary materialThe online version of this article (doi:10.1007/s00429-017-1375-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

et al. 2017

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“…If these studies are in any way comparable, it would appear that there are two separate uptake mechanisms for cadaverine that show nearly identical characteristics, except that they are operative over widely different ranges of concentration. Because cadaverine concentrations in the rodent brain are in the nanomolar range (Stepita-Klauco and Dolezalova, 1974), it appears that the uptake system demonstrated here would be of physiological relevance. An uptake system for cadaverine in the telencephalon, striatum, and hypothalamus might explain the higher concentrations of label seen in these structures in the in vivo experiment.…”

Section: Discussionmentioning

confidence: 86%

“…M . for brain and blood, respectively-Stepita-Klauco and Dolezalova, 1974). The source of cadaverine in the brain may be strictly peripheral, or both peripheral and central.…”

Section: Discussionmentioning

confidence: 99%

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Cadaverine in the Rat Brain: Regional Distribution and Acylation of [¹⁴C]Cadaverine In Vivo and Uptake In Vitro

Salzman

Stepita-Klauco

1981

Journal of Neurochemistry

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The regional distribution and acylation of intraventricularly injected [14C]cadaverine was studied in the rat brain over a 48-h period. The concentrations of labeled cadaverine and its acyl derivatives, N-monoacetylcadaverine and N-monopropionylcadaverine, were determined in the telencephalon, striatum, hypothalamus, midbrain, cerebellum, and medulla-pons by TLC of their 5-dimethylamino-1-naphthalenesulfonyl derivatives, followed by liquid scintillation spectrometry. The apparent passage of radioactivity from the ventricular space into brain tissue was slow, with the concentrations reaching a peak at 24 h after injection. The percentage of radioactivity in the acyl forms of cadaverine, however, was maximal 4 h after injection, with the propionyl form predominating. The telencephalon, striatum, and hypothalamus contained the highest concentrations of radioactivity, in all three forms, at all elapsed times. A high-affinity uptake mechanism for cadaverine was demonstrated in slices of these tissues. This process was completely inhibited by equimolar concentrations of unlabeled putrescine.

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“…diamine is likely to be metabolised from cadaverine through transmethylation, as shown in Figure 7, in a similar way to the production of substances such as N,N-dimethyltryptamine (DMT) [40]. Cadaverine is a malodourous diamine produced by the bacterial decarboxylation of lysine [41] and has been shown to be mildly toxic to rats [42]. In captive cheetahs, the high protein intake from muscle meat, could potentially result in increased lysine being available for colonic bacterial decarboxylation.…”

Section: Resultsmentioning

confidence: 99%

Gas chromatography-mass spectrometry profiles of urinary organic acids in healthy captive cheetahs ( Acinonyx jubatus )

Tordiffe

Reenen

Reyers³

et al. 2017

Journal of Chromatography B

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In captivity, cheetahs (Acinonyx jubatus) frequently suffer from several unusual chronic diseases that rarely occur in their free-ranging counterparts. In order to develop a better understanding of their metabolism and health we documented the urine organic acids of 41 apparently healthy captive cheetahs, in an untargeted metabolomic study, using gas chromatography-mass spectrometry. A total of 339 organic acids were detected and annotated. Phenolic compounds, thought to be produced by the anaerobic fermentation of aromatic amino acids in the distal colon, as well as their corresponding glycine conjugates, were present in high concentrations. The most abundant organic acids in the cheetahs' urine were an as yet unidentified compound and a novel cadaverine metabolite, tentatively identified as N,N-dimethylpentane-1,5-diamine. Pantothenic acid and citramalic acid concentrations correlated negatively with age, while glutaric acid concentrations correlated positively with age, suggesting possible dysregulation of coenzyme A metabolism in older cheetahs. This study provides a baseline of urine organic acid reference values in captive cheetahs and suggests important avenues for future research in this species.

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Cadaverine in the brain of axenic mice

Cited by 27 publications

References 9 publications

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

Cadaverine in the Rat Brain: Regional Distribution and Acylation of [¹⁴C]Cadaverine In Vivo and Uptake In Vitro

Gas chromatography-mass spectrometry profiles of urinary organic acids in healthy captive cheetahs ( Acinonyx jubatus )

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Cadaverine in the brain of axenic mice

Cited by 27 publications

References 9 publications

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

Metabolomics and neuroanatomical evaluation of post-mortem changes in the hippocampus

Cadaverine in the Rat Brain: Regional Distribution and Acylation of [14C]Cadaverine In Vivo and Uptake In Vitro

Gas chromatography-mass spectrometry profiles of urinary organic acids in healthy captive cheetahs ( Acinonyx jubatus )

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Cadaverine in the Rat Brain: Regional Distribution and Acylation of [¹⁴C]Cadaverine In Vivo and Uptake In Vitro