Cadmium accumulation and toxicity in Mytilus edulis: Involvement of metallothioneins and heavy-molecular weight protein

Nolan, Canice; Duke, E. J.

doi:10.1016/0166-445x(83)90052-8

Cited by 45 publications

(11 citation statements)

References 19 publications

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“…Recent studies have identified the presence of metal-binding proteins, similar to metallothioneins, in several tissues of M. edulis (e.g. gills, hepatopancreas, kidney and mantle), following exposure to cadmium (NoEl-Lambot 1976, George et al 1979, Frankenne et al 1980, K6hler and Riisgfird 1982, Nolan and Duke 1983a, b, Frazier et al 1985, copper (Harrison et al 1988), mercury (Roesijadi etal. 1982, Roesijadi 1986) and silver (George etal.…”

Section: Introductionmentioning

confidence: 97%

Metallothionein induction inMytilus edulis exposed to cadmium

1991

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Abstract. The exposure of mussels, Mytilus edulis, collected from Whitsand Bay, southwest England, in August 1988, to sublethal concentrations of cadmium (400/~g 1-i) for 65 d resulted in the induction of metallothionein (MT) synthesis in the soft tissues. In cadmium-exposed mussels, metallothionein concentrations, measured by differential pulse polarography, increased by a factor of three, from 2 to 3 mg g-1 to a maximum of 9 mg g-1 after 30 d. No significant changes could be detected in controls. Cadmium accumulated in the soft tissues of mussels correlated significantly with metallothionein concentrations and can be described by the relationship: MT (mg g-1) =0.045 Cd (#g g-1)+ 3.03 (r=0.803, P<0.001). Gel chromatography of heat-treated cytosolic extracts showed that the accumulated cadmium is bound principally to the newly formed metallothioneins. Copper and zinc were also analysed in the whole soft-tissues and in subcellular fractions of cadmium-exposed mussels. Although copper concentrations were not affected by cadmium-exposure, zinc levels were significantly reduced. The results demonstrate that the induction of metallothioneins in M. edulis is a quantifiable biological response to sublethal levels of cadmium exposure.

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Section: Introductionmentioning

confidence: 97%

Metallothionein induction inMytilus edulis exposed to cadmium

1991

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“…Biochemical studies have also identified the presence of metal binding proteins, similar to metallothioneins, in several tissues of M. edulis and M. galloprovincialis (e.g. gills, hepatopancreas, digestive gland, mantle and kidney) following exposure to cadmium (Noel-Lambot 1976, George & Pirie 1979, Frankenne et al 1980, Kohler & Riisgard 1982, Carpene et al 1983, Nolan & Duke 1983a,b, Frazier et al 1985, Viarengo et al 1985b, Pavicic et al 1987.…”

Section: Introductionmentioning

confidence: 99%

Turnover rate of metallothionein and cadmium in Mytilus edulis

Bebianno

Langston

1993

Biometals

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The results demonstrate the first attempt to determine metallothionein turnover in the whole soft tissues of mussels Mytilus edulis exposed to cadmium. Half-lives for metallothionein and cadmium are 25 and 300 days, respectively. As metallothionein degrades the released cadmium induces further synthesis of the protein, to which the metal becomes resequestered. The slow metallothionein turnover rates (compared with mammals) and the lack of significant cadmium excretion testify to the relatively stable nature of the cadmium-metallothionein complex in these invertebrates and supports the view of a detoxifying role for metallothionein in the mussels.

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“…Numerous studies of representative bivalve species have shown that, in addition to the digestive gland, the gills, mantle, and other organs can be involved in the accumulation of heavy metals (Bebiano et al 1994;Nolan and Duke 1983). The Pectinidae family representatives accumulate cadmium primarily in the digestive gland, regardless of the source of cadmium input (sea water, soil or food).…”

Section: Discussionmentioning

confidence: 99%

Metallothionein-like proteins induced by cadmium stress in the scallop Mizuhopecten yessoensis

et al. 2012

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Organisms have evolved a cellular response called stress protein response that increases their tolerance in adverse environmental conditions. Well known stress proteins that bind essential and toxic metals are metallothionein (MT). The scallop Mizuhopecten yessoensis is the most interesting organism because it is able to accumulate toxic cadmium in its digestive gland. However, in the tissue of the digestive gland of Mizuhopecten yessoensis MT (metallothioneins) have not been found. Eastern scallops, Mizuhopecten yessoensis, were collected from two locations -one clean and one polluted site. The concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were measured in the digestive gland. There was a significant increase in Cd concentrations in this studied tissue. We found that in the presence of cadmium Mizuhopecten yessoensis can induce high molecular proteins. The results of experiments have shown that Cd-binding ligands have a number of properties similar to MT: acetone and temperature stability; the ability to bind some metals, including Cd, Cu and Zn. Protein chromatography (FPLC, Superosa 12) from the digestive gland of scallop M. yessoensis has shown that cadmium is associated with high molecular weight Cd-binding proteins (72 kDa and 43 kDa). The major cadmium-binding protein 72 kDa is glycoprotein. In experiments we have demonstrated that Cd-binding proteins can be induced when there is cadmium exposure. The results of this study strongly suggest that the far eastern scallop Mizuhopecten yessoensis has a unique and well-developed system for the detoxification of heavy metals and it allows for biochemical systems to be maintained in a relatively stable manner in the presence of heavy metals.

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Cadmium accumulation and toxicity in Mytilus edulis: Involvement of metallothioneins and heavy-molecular weight protein

Cited by 45 publications

References 19 publications

Metallothionein induction inMytilus edulis exposed to cadmium

Metallothionein induction inMytilus edulis exposed to cadmium

Turnover rate of metallothionein and cadmium in Mytilus edulis

Metallothionein-like proteins induced by cadmium stress in the scallop Mizuhopecten yessoensis

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