Cadmium and copper toxicity in three marine macroalgae: evaluation of the biochemical responses and DNA damage

Babu, M. Yokesh; Palanikumar, L.; Nagarani, N.; Devi, V. Janaki; Kumar, Sanoj; Ramakritinan, C.M.; Kumaraguru, A. K.

doi:10.1007/s11356-014-2999-0

Cited by 47 publications

(10 citation statements)

References 55 publications

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“…Elevated levels of antioxidant enzymes SOD, CAT, GR and POD in C . vulgaris following the Cd treatment in this study indicated that these enzymes could act in combination to reduce the impact of metal toxicity, the same results has reported in Acanthophora spicifera , Chaetomorpha antennina , and Ulva reticulate (Babu et al 2014 ).…”

Section: Discussionsupporting

confidence: 88%

“…Therefore, the increased glutathione level noted in C. vulgaris when treated with Cd under 0–1 mg L −1 , that may precede phytochelatin accumulation by intracellular sequestration of metal ions (De Vos et al 1992 ). And the decreased glutathione level showed in C. vulgaris when treated with Cd under 3–7 mg L −1 , similarly, exposure of Cd decreased glutathione of A. spicifera and exposure of Cu decreased glutathione of C. antennina (Babu et al 2014 ).…”

Section: Discussionmentioning

confidence: 86%

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The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

et al. 2016

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The objective of the present work was to evaluate the effect of exogenously applied cadmium on the physiological response of green algae Chlorella vulgaris. The study investigated the long-term effect (18 days) of cadmium on the levels of algae biomass, assimilation pigment composition, soluble protein, oxidative status (production of hydrogen peroxide and superoxide anion), antioxidant enzymes (such as superoxide dismutase, peroxidase, catalase and glutathione reductase enzyme) in C. vulgaris. The results showed that growth, the amount of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids gradually decreased with increasing cadmium over 18 days exposure. Cadmium at concentration of 7 mg L−1 inhibited algal growth expressed as the number of cells. Our research found that C. vulgaris has a high tolerance to cadmium. Contents of chlorophylls (Chl a and Chl b) and carotenoids (Car) of C. vulgaris was significantly decline with rising concentration of cadmium (p < 0.05). The decrease of 54.04 and 93.37 % in Chl a, 60.65 and 74.32 % in Chl b, 50.00 and 71.88 % in total carotenoids was noticed following the treatment with 3 and 7 mg L−1 cadmium doses compared with control treatment, respectively. Cadmium treatments caused a significant change in the physiological competence (calculated as chlorophyll a/b) which increased with increasing Cd(II) doses up to 1 mg L−1 but decreased at 3 mg L−1. While accumulation of soluble protein was enhanced by presence of cadmium, the treatment with cadmium at 3 and 7 mg L−1 increased the concentration of soluble proteins by 88, 95.8 % in C. vulgaris, respectively. Moreover, low doses of cadmium stimulated enzymatic (superoxide dismutase, catalase and glutathione reductase) in C. vulgaris, The content of peroxidase increased with the increasing cadmium concentration, and had slightly decreased at the concentration of 7 mg L−1, but was still higher than control group, which showed that cadmium stress at high concentration mainly peroxidase works in C. vulgaris. And therefore, suppressed reactive oxygen species (hydrogen peroxide and superoxide) accumulated. The present study also showed that cadmium increased oxidative stress and induced antioxidant defense systems against reactive oxygen species. The observation in here analyzed C. vulgaris after exposure to cadmium indicate that hydrogen peroxide, superoxide and peroxidase in the alga with exposure to Cd(II) seemed to be parameters as biomarkers for metal-induced oxidative stress.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 86%

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

et al. 2016

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“…With the increase of the heavy metal concentration, membrane lipid peroxidation of the algae cell wall by Zn 2+ has exceeded the repair capacity by the organism. The MDA in Ulva reticulata and Acanthophora spicifera species increased gradually with increasing concentrations of Cu and Cd (Babu et al 2014). Thus, exposure to Cu 2+ and Zn 2+ induces MDA in Cladophora.…”

Section: Effects Of Cu 2+ and Zn 2+ On The Malondialdehyde Of Cladophoramentioning

confidence: 92%

Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

Cao

Xie

Deng

et al. 2015

Environ Sci Pollut Res

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Effects of various concentrations of Cu(2+) and Zn(2+) (0.0, 0.1, 0.25, 0.5, or 1.0 mg/L) on the growth, malondialdehyde (MDA), the intracellular calcium, and physiological characteristics of green algae, Cladophora, were investigated. Low Zn(2+) concentrations accelerated the growth of Cladophora, whereas Zn(2+) concentration increases to 0.25 mg/L inhibited its growth. Cu(2+) greatly influences Cladophora growth. The photosynthesis of Cladophora decreased under Zn(2+) and Cu(2+) stress. Cu(2+) and Zn(2+) treatment affected the content of total soluble sugar in Cladophora and has small increases in its protein content. Zn(2+) induced the intracellular calcium release, and copper induced the intracellular calcium increases in Cladophora. Exposure to Cu(2+) and Zn(2+) induces MDA in Cladophora. The stress concent of Cu(2+) was strictly correlated with the total soluble sugar content, Chla+Chlb, and MDA in Cladophora, and the stress concent of Zn(2+) was strictly correlated with the relative growth rate (RGR) and MDA of Cladophora.

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“…Cadmium toxicity also involves the binding of Cd 2? to sulfhydryl groups on critical molecules, thiol group inactivation, increased ROS levels, lipid peroxidation [3], DNA damage [4], inhibition protein synthesis [5], reduction of the activity of the antioxidant enzymes, mitochondrial damage and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of l-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway

et al. 2015

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L-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of L-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with L-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that L-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, L-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, L-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, L-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that L-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that L-theanine could be exploited for prevention of cadmium-induced diseases.

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Cadmium and copper toxicity in three marine macroalgae: evaluation of the biochemical responses and DNA damage

Cited by 47 publications

References 55 publications

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris

Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

Protective Effect of l-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway

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