Cadmium- and iron-stress-inducible gene expression in the green alga Chlamydomonas reinhardtii : evidence for H43 protein function in iron assimilation

Rubinelli, Peter M.; Siripornadulsil, Surasak; Gao-Rubinelli, Fei; Sayre, Richard T.

doi:10.1007/s00425-001-0711-3

Cited by 81 publications

(48 citation statements)

References 39 publications

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“…The AR has been observed in bacteria (Assis et al, 2002;Sedgwick & Lindahl, 2002;Rohankhedkar et al, 2006), yeast (Boreham & Mitchel, 1991;Gajendiran & Jeevanram, 2002), algae (Chankova & Bryant, 2001;Rubinelli et al, 2002;Chankova et al, 2007), insect cells (Savina et al, 2003), mammalian cells (Wang & Cai, 2000;Tiku & Kale, 2001;Ulsh et al, 2004;Zhou et al, 2004), human cells (Schlade-Bartusiak et al, 2002;Atanasova et al, 2005;Coleman et al, 2005;Friesner et al, 2005;Lanza et al, 2005;Seo et al, 2006) and higher plants models (Rieger et al, 1993;Panda et al, 1997;Jovtchev & Stergios, 2003;Patra et al, 2003). A study of the conditions essential for the induction of an adaptive response is of critical importance to understanding the novel biological defense mechanisms against the hazardous effects of radiation.…”

Section: Hormesis and Molecular Mechanisms Of The Adaptive Responsementioning

confidence: 99%

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

Iglesias-Andreu¹,

Octavio‐Aguilar²,

Bello-Bello³

2012

Gamma Radiation

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Section: Hormesis and Molecular Mechanisms Of The Adaptive Responsementioning

confidence: 99%

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

Iglesias-Andreu¹,

Octavio‐Aguilar²,

Bello-Bello³

2012

Gamma Radiation

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“…The homologue corresponds to protein ID 173281, which is immediately adjacent to H43 (protein ID 129929 in version 3.0 of the genome). Rubinelli et al had previously identified the H43 gene in a screen for genes displaying differential expression under cadmium treatment (74). Nevertheless, they suggested that the gene encodes an iron assimilation component based on its increased expression in iron-deficient Chlamydomonas and its ability to stimulate the growth of a fet3 fet4 strain of Saccharomyces cerevisiae.…”

mentioning

confidence: 99%

FEA1 , FEA2 , and FRE1 , Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with FOX1 and FTR1 in Iron-Deficient Chlamydomonas reinhardtii

et al. 2007

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Previously, we had identified FOX1 and FTR1 as iron deficiency-inducible components of a high-affinity copper-dependent iron uptake pathway in Chlamydomonas. In this work, we survey the version 3.0 draft genome to identify a ferrireductase, FRE1, and two ZIP family proteins, IRT1 and IRT2, as candidate ferrous transporters based on their increased expression in iron-deficient versus iron-replete cells. In a parallel proteomic approach, we identified FEA1 and FEA2 as the major proteins secreted by iron-deficient Chlamydomonas reinhardtii. The recovery of FEA1 and FEA2 from the medium of Chlamydomonas strain CC425 cultures is strictly correlated with iron nutrition status, and the accumulation of the corresponding mRNAs parallels that of the Chlamydomonas FOX1 and FTR1 mRNAs, although the magnitude of regulation is more dramatic for the FEA genes. Like the FOX1 and FTR1 genes, the FEA genes do not respond to copper, zinc, or manganese deficiency. The 5 flanking untranscribed sequences from the FEA1, FTR1, and FOX1 genes confer iron deficiency-dependent expression of ARS2, suggesting that the iron assimilation pathway is under transcriptional control by iron nutrition. Genetic analysis suggests that the secreted proteins FEA1 and FEA2 facilitate high-affinity iron uptake, perhaps by concentrating iron in the vicinity of the cell. Homologues of FEA1 and FRE1 were identified previously as high-CO 2 -responsive genes, HCR1 and HCR2, in Chlorococcum littorale, suggesting that components of the iron assimilation pathway are responsive to carbon nutrition. These iron response components are placed in a proposed iron assimilation pathway for Chlamydomonas.Although iron is abundant in soil, its bioavailability in the aerobic world is poor because of the relative insolubility of Fe(III) and the tight binding of iron to organic chelators (reviewed in references 33, 44, and 56). Iron is therefore one of the essential nutrients that limits virtually all forms of life, and its assimilation involves mechanisms for iron capture from otherwise inaccessible forms plus mechanisms for transport. Many organisms, especially microbes, use multiple pathways for iron uptake because of the varied forms and amounts of iron supply in nature. At the same time, because of its propensity for redox chemistry, especially in an aerobic environment, iron can be toxic to cells. Therefore, the metabolism of iron is very tightly regulated (reviewed in references 9, 13, 22, 41, and 75).Fungi. Because of the importance of iron nutrition to life, the mechanisms of iron assimilation and distribution have been studied in many eukaryotic organisms. One well-studied pathway for iron assimilation was discovered initially through studies of iron metabolism in Saccharomyces cerevisiae. In this pathway, iron is first solubilized by reduction of ferric to ferrous iron and is then available for uptake by high-affinity transporters such as the Fet3p/Ftr1p complex (3,14,27,49,52,72,81,92). There are several transcriptionally regulated FRE genes with different substr...

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“…This suggests that the H43/FEA1 orthologs may be widely distributed among at least chlorophyte algae. The function of H43/FEA1 is not completely understood but one possible role may be in iron assimilation (Allen et al, 2007;Rubinelli et al, 2002). Allen et al (2007) identified FEA1, FEA2, and a candidate ferrireductase (FRE1) are expressed coordinately with iron assimilation components, and it was hypothesized that the proteins may facilitate iron uptake with high affinity by concentrating iron in the vicinity of the cells (Allen et al, 2007).…”

Section: High-co 2 Signalingmentioning

confidence: 99%

“…The signal for H43/FEA1 expression might be sensed by putative proteins localized on the cell membrane, which are influenced by protein modifiers and send the signal for H43/FEA1 expression . H43/FEA1 expression is induced under excessive levels of Cd (>25 μM) or iron-deficient conditions (<1 μM) (Allen et al, 2007;Rubinelli et al, 2002). Fei et al (2009) reported two transcriptional cis-elements that are responsive to the Fe-deficient signal (FeREs) for H43/FEA1 expression, namely FeRE1 and FeRE2, which are located at -273/-259 and -106/-85 upstream from the H43/FEA1 transcriptional initiation site.…”

Section: High-co 2 Signalingmentioning

confidence: 99%

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High-CO2 Response Mechanisms in Microalgae

Baba¹,

Shiraiwa²

2012

Advances in Photosynthesis - Fundamental Aspects

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Cadmium- and iron-stress-inducible gene expression in the green alga Chlamydomonas reinhardtii : evidence for H43 protein function in iron assimilation

Cited by 81 publications

References 39 publications

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

Current Importance and Potential Use of Low Doses of Gamma Radiation in Forest Species

FEA1 , FEA2 , and FRE1 , Encoding Two Homologous Secreted Proteins and a Candidate Ferrireductase, Are Expressed Coordinately with FOX1 and FTR1 in Iron-Deficient Chlamydomonas reinhardtii

High-CO2 Response Mechanisms in Microalgae

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