Cadmium chloride-induced DNA and lysosomal damage in a hepatoma cell line

Fotakis, George; Cemeli, Eduardo; Anderson, Diana; Timbrell, John A.

doi:10.1016/j.tiv.2005.02.001

Cited by 91 publications

(45 citation statements)

References 50 publications

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“…Accumulation of cadmium in the kidney and liver disturbs the function of these organs, which is accompanied by an increase in the excretion of lysosomal enzymes (Järup et al, 1998;Fotakis et al, 2005;Thomas et al, 2009). This phenomenon can be explained by irreversible damage at the cellular level, where mitochondria are injured and the electron transfer chain is inhibited (Wang et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Cells of the proximal convoluted tubules are very sensitive to hypoxia, which leads to their dysfunction (Haase, 2013). On the other hand, the impact of cadmium on lysosomes, despite its multiplexing, can lead to lysosomal membrane destabilisation, resulting in an efflux of lysosomal enzymes into body fluids (blood, urine) (Fotakis et al, 2005;Thomas et al, 2009). This is confirmed by our unpublished results obtained in electron microscopic (TEM) studies on organs of chicks treated with Cd in ovo.…”

Section: Discussionmentioning

confidence: 99%

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Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata)

Dżugan

Lis

2016

Acta Veterinaria Hungarica

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The aim of this study was to determine the effect of cadmium on Muscovy ducklings (Cairina moschata) based on hatching results and the activity of enzymes in the blood plasma. On day 6 of incubation, hatching eggs were injected into the egg albumen with 50 µl of saline solution containing Cd ions (CdCl 2 ) at concentrations of 0 (control group), 1.3, 4.0, 7.5, 15.0 and 30 µg/egg, using 50 eggs per group. A gradual decrease in hatchability, from 52% in the control to 4% in the highest Cd dose group, was observed, with the LD 50 calculated as 8 μg/egg. However, the impact of cadmium on the incidence of malformations of duck embryos has not been proven. Compared to the control group, N-acetyl-β-Dglucosaminidase activity increased by 30-50% (P ≤ 0.05) in the blood serum of ducklings in the groups receiving more than 7.5 µg Cd/egg, whereas an elevated activity of arylsulphatase (by 45%) was observed for a lower dose only (4 µg Cd/egg). A gradual increase in the activity of alanine and aspartate aminotransferases was observed (P ≤ 0.05), starting from the lowest exposure of 1.3 µg Cd/egg, by 155% and 53%, respectively. In conclusion, the results prove the dosedependent toxic impact of cadmium on embryogenesis and on the studied blood plasma enzyme activities of ducklings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata)

Dżugan

Lis

2016

Acta Veterinaria Hungarica

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“…However, it is suggested that generation of an oxidative stress in the cell plays a central role (41,44). Although cadmium is not a Fenton metal and thus does not directly generate reactive oxygen species (ROS) (32), a number of studies show increased ROS production in cadmium-treated cells (19,38). Consistently, in many cases, free radical scavengers or antioxidants were shown to lessen cadmium toxicity (8,17,45).…”

mentioning

confidence: 99%

Redox Regulation of Human OGG1 Activity in Response to Cellular Oxidative Stress

Bravard

Vacher

Gouget

et al. 2006

Molecular and Cellular Biology

144

108

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8-Oxoguanine (8-oxoG), a common and mutagenic form of oxidized guanine in DNA, is eliminated mainly through base excision repair. In human cells its repair is initiated by human OGG1 (hOGG1), an 8-oxoG DNA glycosylase. We investigated the effects of an acute cadmium exposure of human lymphoblastoid cells on the activity of hOGG1. We show that coinciding with alteration of the redox cellular status, the 8-oxoG DNA glycosylase activity of hOGG1 was nearly completely inhibited. However, the hOGG1 activity returned to normal levels once the redox cellular status was normalized. In vitro, the activity of purified hOGG1 was abolished by cadmium and could not be recovered by EDTA. In cells, however, the reversible inactivation of OGG1 activity by cadmium was strictly associated with reversible oxidation of the protein. Moreover, the 8-oxoG DNA glycosylase activity of purified OGG1 and that from crude extracts were modulated by cysteine-modifying agents. Oxidation of OGG1 by the thiol oxidant diamide led to inhibition of the activity and a protein migration pattern similar to that seen in cadmiumtreated cells. These results suggest that cadmium inhibits hOGG1 activity mainly by indirect oxidation of critical cysteine residues and that excretion of the metal from the cells leads to normalization of the redox cell status and restoration of an active hOGG1. The results presented here unveil a novel redox-dependent mechanism for the regulation of OGG1 activity.

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“…Nanoparticles, such as silver nanoparticles, are showing severe toxic effects on the male reproductive system. The identified research suggests that nanoparticles cross the blood-testes barrier and are deposited in the testes, and that there is potential for adverse effects on sperm cells [29]. In the literature of Burd et al, it was demonstrated that commercially available silver-based dressings are also showing potential cytotoxic effects.…”

Section: Ethical Issues In Nanomedicinementioning

confidence: 99%

The Question of Ethics in Nanomedicine

Kazemi¹,

Majidinia²,

Jamali³

2014

J Clinic Res Bioeth

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Lately, nanotechnology and nanomedicine have achieved tremendous interest and funding, from several entities and directions. Nanomedicine demonstrates great promise for development of medical diagnosis, treatment, and prevention; it also raises various ethical concerns however, there has not been so much study and discussion about social and ethical issues. Nanomaterials are extremely reactive in biological systems owing to the large surface area. Although the benefits of nanomaterials are apparent, there are some studies which show the potential risk to biological systems. Therefore, the rapid progress of nanomedicine and its potentially profound social and environmental impacts is indicative of how the arisen problems could be dangerous and already there are signs that some challenging ethical issues could be raised. In this paper we will examine more recent developments in debates on nanomedicine ethics.

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Cadmium chloride-induced DNA and lysosomal damage in a hepatoma cell line

Cited by 91 publications

References 50 publications

Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata)

Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata)

Redox Regulation of Human OGG1 Activity in Response to Cellular Oxidative Stress

The Question of Ethics in Nanomedicine

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