Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis

Wang, Hui; Liu, Zheng; Zhang, Wenxiu; Yuan, Ziao; Yuan, Hongbin; Liu, Xueting; Yang, Chunwen; Guan, Weijun

doi:10.1186/s40659-016-0103-6

Cited by 18 publications

(6 citation statements)

References 44 publications

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“…Moreover, since heavy metal exposure can cause great harm to Siberian tigers (Panthera tigris altaica) in a natural environment, the comet assay was applied in vitro for the evaluation of cadmium-induced DNA damage in tiger fibroblasts. Results showed DNA-damaging effects in cadmium-treated cells and this could later on serve in developing interventions to treat and prevent cadmium poisoning [433]. The comet assay was also done on several species of monkeys.…”

Section: Wildlife Mammalsmentioning

confidence: 98%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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Section: Wildlife Mammalsmentioning

confidence: 98%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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“…Cd causes serious harm to humans and livestock when it becomes bio-magnified in food webs. There have been reports of Cd contamination events in recent years worldwide 2 , 3 . Our laboratory has long been committed to investigating the mechanism of cadmium toxicity.…”

Section: Introductionmentioning

confidence: 99%

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Luo

Yuan

et al. 2017

Sci Rep

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Parthanatos is a newly discovered form of PARP-1-dependent programmed cell death. It has been reported to play an important role in several cancer or tumour cells; however, few studies have been performed in normal cells. Cadmium is a highly toxic pollutant and is reported to induce autophagy and apoptosis in multiple cell types. Although cadmium toxicity induces cell death, the underlying mechanism is not fully understood. Therefore, in this study we aimed to investigate the mechanism of Cadmium -induced cell damage using rat proximal tubular cell line NRK-52E and primary rat proximal tubular (rPT) cells. Our results indicated that parthanatos and the MAPK signalling pathway contribute to Cadmium-induced cell death, and that oxidative stress and mitochondrial damage play key roles in this process. In addition, parthanatos with oxidative stress has a synergistic effect on apoptosis, and JNK1/2 and p38 contribute to parthanatos.

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“…In addition, the level of tetrahydrocorticosterone in the metabolic pathway of steroidogenesis was significantly downregulated in the S3 compared with the S0, and suggested BMFA could effectively reduce production of the reactive oxygen species (ROS) and then relieve the oxidative stress in the gut ( 34 , 35 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of blended microbial feed additives on performance, meat quality, gut microbiota and metabolism of broilers

Zhou

Hou

et al. 2022

Front. Nutr.

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The present study investigated the effects of blend microbial feed additive (BMFA) in diet on performance, meat quality, gut microbiota and metabolism of broilers. In this study 240 seventy-day-old female Wenchang broilers were randomly allocated into four groups with five replicates of 12 broilers each. Broilers in the control group was fed only basal diet (S0), and the other three groups were fed the same basal diet supplemented with 0.2% (S1), 0.4% (S2), or 0.6% (S3) of BMFA, respectively. The trial continued for 54 days. The results showed that broilers in S2 and S3 had lower average daily feed intake (ADFI) compared with S0 and S1 (P < 0.05). However, diet supplementation with BMFA had no significantly influence on the average daily gain (ADG) and the ratio of ADFI to ADG (F/G) (P > 0.05). The highest thigh muscle percentage was observed in S2 (P < 0.05) among all groups. Diet supplementation with BMFA reduced the shear force in both breast and thigh muscles (P < 0.05) of broilers. An increase (P < 0.05) in the total unsaturated fatty acid (USFA), monounsaturated fatty acids (MUFA), and ratio of unsaturated fatty acids to saturated fatty acid (USFA/SFA) in breast muscles was observed in S3 compared with S0. It was found that the S3 had a relatively higher abundance of Lactobacillus (P < 0.001), as well as a lower abundance of the Bacteroides, Rikenellaceae RC9 gut group, Olsenella, Prevotellaceae UCG-001 and Prevotella (P < 0.05) than the S0. Correlation analysis indicated that a total of 17 differential metabolites between the S3 and S0 were significantly correlated with the 7 differential genera microflora. Overall, diet supplementation with 0.6% of BMFA can significantly improve the meat quality of broilers by decreasing the concentration of SFA and enhancing the levels of the total USFA, MUFA and USFA/SFA in breast muscles. Those findings were tightly bound to the higher proportion of Lactobacillus genus in the intestinal tract of broilers influenced by BMFA.

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Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis

Cited by 18 publications

References 44 publications

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Effects of blended microbial feed additives on performance, meat quality, gut microbiota and metabolism of broilers

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