Cadmium-induced changes in the membrane of human erythrocytes and molecular models

Suwalsky, Mario; Villena, Fernando Pardo-Manuel de; Norris, Beryl; Cuevas, Francisco; Sotomayor, Carlos P.

doi:10.1016/j.jinorgbio.2004.02.027

Cited by 34 publications

(16 citation statements)

References 24 publications

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“…binding to erythrocytes has been shown before by electron microscopy (Hg 2? concentration in low mM range) (Suwalsky et al 2004) and by NMR spectroscopy (metal concentration in the 100 mM range) (Delnomdedieu et al 1992). Therefore, the developed biomimetic system is a useful model to study metal-lipid interactions by fluorescence spectroscopy in vitro.…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…In contrast, X-ray diffraction studies revealed that 10 lM Cd 2? affected PC membranes and this metal was shown to demolish bilayer structures at a concentration of 1 mM (Suwalsky et al 2004). These authors also reported smaller effects of Cd 2?…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…These authors also reported smaller effects of Cd 2? on PE (Suwalsky et al 2004), which may reflect the fact that PE membranes are more rigid than the respective PC membranes. Cd 2?…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…These observations are consistent with previous studies which demonstrated that Cd 2? will induce lipid disordering in isolated erythrocyte membranes above *100 lM (Suwalsky et al 2004).…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…Moreover, Cd 2? has been demonstrated to decrease the fluidity of lipid headgroups and acyl chain regions in both human erythrocytes and dimyristoylphosphatidylcholine liposomes (Suwalsky et al 2004), which was rationalized in terms of the electrostatic binding of Cd 2? to the phosphate groups of the lipids (Girault et al 1998).…”

Section: Introductionmentioning

confidence: 95%

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Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

Gailer

Prenner

2008

Biometals

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In order to characterize the potentially deleterious effects of toxic Hg(2+) and Cd(2+) on lipid membranes, we have studied their binding to liposomes whose composition mimicked erythrocyte membranes. Fluorescence spectroscopy utilizing the concentration dependent quenching of Phen Green SK by Hg(2+) and Cd(2+) was found to be a sensitive tool to probe these interactions at metal concentrations < or =1 microM. We have systematically developed a metal binding affinity assay to screen for the interactions of Hg(2+) or Cd(2+) with certain lipid classes. A biomimetic liposome system was developed that contained four major lipid classes of erythrocyte membranes (zwitterionic lipids: phosphatidylcholine and phosphatidylethanolamine; negatively charged: phosphatidylserine and neutral: cholesterol). In contrast to Hg(2+), which preferentially bound to the negatively charged phosphatidylserine compared to the zwitterionic components, Cd(2+) bound stronger to the two zwitterionic lipids. Thus, the observed distinct differences in the binding affinity of Hg(2+) and Cd(2+) for certain lipid classes together with their known effects on membrane properties represent an important first step toward a better understanding the role of these interactions in the chronic toxicity of these metals.

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Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…These authors also reported smaller effects of Cd 2? on PE (Suwalsky et al 2004), which may reflect the fact that PE membranes are more rigid than the respective PC membranes. Cd 2?…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

“…These observations are consistent with previous studies which demonstrated that Cd 2? will induce lipid disordering in isolated erythrocyte membranes above *100 lM (Suwalsky et al 2004).…”

Section: Assessment Of Metal Binding Affinity To Liposomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

Gailer

Prenner

2008

Biometals

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Aluminum increases toxic effects of amyloid β-peptides on the human erythrocyte membrane and molecular models

Suwalsky

Hernández

Sotomayor

2012

Metal Ions in Neurological Systems

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Initial autophagic protection switches to disruption of autophagic flux by lysosomal instability during cadmium stress accrual in renal NRK-52E cells

et al. 2017

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The renal proximal tubule (PT) is the major target of cadmium (Cd) toxicity where Cd causes stress and apoptosis. Autophagy is induced by cell stress, e.g., endoplasmic reticulum (ER) stress, and may contribute to cell survival or death. The role of autophagy in Cd-induced nephrotoxicity remains unsettled due to contradictory results and lack of evidence for autophagic machinery damage by Cd. Cd-induced autophagy in rat kidney PT cell line NRK-52E and its role in cell death was investigated. Increased LC3-II and decreased p62 as autophagy markers indicate rapid induction of autophagic flux by Cd (5-10 µM) after 1 h, accompanied by ER stress (increased p-PERK, p-eIF2α, CHOP). Cd exposure exceeding 3 h results in p62/LC3-II accumulation, but diminished effect of lysosomal inhibitors (bafilomycin A1, pepstatin A +E-64d) on p62/LC3-II levels, indicating decreased autophagic flux and cargo degradation. At 24 h exposure, Cd (5-25 µM) activates intrinsic apoptotic pathways (Bax/Bcl-2, PARP-1), which is not evident earlier (≤6 h) although cell viability by MTT assay is decreased. Autophagy inducer rapamycin (100 nM) does not overcome autophagy inhibition or Cd-induced cell viability loss. The autophagosome-lysosome fusion inhibitor liensinine (5 μM) increases CHOP and Bax/Bcl-2-dependent apoptosis by low Cd stress, but not by high Cd. Lysosomal instability by Cd (5 μM; 6 h) is indicated by increases in cellular sphingomyelin and membrane fluidity and decreases in cathepsins and LAMP1. The data suggest dual and temporal impact of Cd on autophagy: Low Cd stress rapidly activates autophagy counteracting damage but Cd stress accrual disrupts autophagic flux and lysosomal stability, possibly resulting in lysosomal cell death.

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Cadmium-induced changes in the membrane of human erythrocytes and molecular models

Cited by 34 publications

References 24 publications

Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

Hg2+ and Cd2+ interact differently with biomimetic erythrocyte membranes

Aluminum increases toxic effects of amyloid β-peptides on the human erythrocyte membrane and molecular models

Initial autophagic protection switches to disruption of autophagic flux by lysosomal instability during cadmium stress accrual in renal NRK-52E cells

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