Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba

Ünyayar, Serpil; Çelık, Ayla; Çekiç, Fazilet Özlem; Gözel, Ayşin

doi:10.1093/mutage/gel001

Cited by 225 publications

(101 citation statements)

References 44 publications

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“…Moreover, Cd can induce oxidative stress and can damage biological macromolecules such as lipids, proteins and DNA through reactive oxygen species (ROS) (5). In plants, Cd induces DNA damage (6) directly or indirectly by inducing oxidative stress (7). Cd can also affect the photosynthetic apparatus (8,9), as well as the respiratory and nitrogen metabolism, which results in growth retardation, leaf chlorosis, water and nutrient imbalances, and the production of ROS (3).…”

mentioning

confidence: 99%

Effect of Copper on the Toxicity and Genotoxicity of Cadmium in Duckweed (Lemna Minor L.)

Cvjetko

Tolić

Šikić

et al. 2010

Archives of Industrial Hygiene and Toxicology

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We investigated interactions between copper (in the concentrations of 2.5 µmol L -1 and 5 µmol L -1 ) and cadmium (5 µmol L -1 ) in common duckweed (Lemna minor L.) by exposing it to either metal or to their combinations for four or seven days. Their uptake increased with time, but it was lower in plants treated with combinations of metals than in plants treated with either metal given alone. In separate treatments, either metal increased malondialdehyde (MDA) level and catalase and peroxidase activity. Both induced DNA damage, but copper did it only after 7 days of treatment. On day 4, the combination of cadmium and 5 µmol L -1 copper additionally increased MDA as well as catalase and peroxidase activity. In contrast, on day 7, MDA dropped in plants treated with combinations of metals, and especially with 2.5 µmol L -1 copper plus cadmium. In these plants, catalase activity was higher than in copper treated plants. Peroxidase activity increased after treatment with cadmium and 2.5 µmol L -1 copper but decreased in plants treated with cadmium and 5 µmol L -1 copper. Compared to copper alone, combinations of metals enhanced DNA damage after 4 days of treatment but it dropped on day 7. In conclusion, either metal given alone was toxic/ genotoxic and caused oxidative stress. On day 4 of combined treatment, the higher copper concentration was more toxic than either metal alone. In contrast, on day 7 of combined treatment, the lower copper concentration showed lower oxidative and DNA damage. These complex interactions can not be explained by simple antagonism and/or synergism. Further studies should go in that direction.

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confidence: 99%

Effect of Copper on the Toxicity and Genotoxicity of Cadmium in Duckweed (Lemna Minor L.)

Cvjetko

Tolić

Šikić

et al. 2010

Archives of Industrial Hygiene and Toxicology

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“…V. faba is not known as a metal tolerant plant. To the contrary, its sensitivity to metals justified its use in ecotoxicity tests (Cordova Rosa et al, 2003;Ünyayar et al, 2006) as it seems to be one of the most metal sensitive plant species (Rahoui et al, 2008). Z. mays plant appeared to be a good candidate for phytoextraction.…”

Section: Earthworm and Plants Interactionmentioning

confidence: 99%

Earthworms Eisenia fetida affect the uptake of heavy metals by plants Vicia faba and Zea mays in metal-contaminated soils

Lemtiri

Liénard

Alabi

et al. 2016

Applied Soil Ecology

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A B S T R A C TEarthworms increase the availability of heavy metals in some situations and aid in maintaining the structure and quality of soil. The introduction of earthworms into metal-contaminated soils has been suggested as an aid for phytoremediation processes. In Wallonia, Belgium, a century of industrial metallurgic activities has led to the substantial pollution of soils by heavy metals, including copper (Cu), zinc (Zn), lead (Pb) and cadmium (Cd), due to atmospheric dusts. Two plant species, Vicia faba and Zea mays, and earthworms (Eisenia fetida) (Savigny, 1826) were exposed to different concentrations of longterm-contaminated soils for 42 days. The soil samples, which were collected from the land surrounding a former Zn-Pb ore-treatment plant, exhibited different levels of heavy metals. Our aim was to evaluate the role of earthworms E. fetida on the availability of metals in soils and their effects on metal uptake by V. faba and Z. mays plants at different soil concentrations.The results suggest that earthworms and plants modified the availability of metals in contaminated soils after 42 days of exposure. Earthworm life-cycle parameters were affected by metal contamination and/or the addition of plants; cocoon production and weight were more responsive to adverse conditions than earthworm survival or weight change. The concentrations of Pb and Cd in earthworm tissues decreased in the presence of plants. Results showed that metal accumulation in plants depended on the metal element considered and the presence of earthworms. However, the presence of earthworms did not change the concentrations of metals in plants, except for Cd. In the presence or absence of earthworms, V. faba accumulated higher concentrations of Cu and Zn compared with Z. mays, which accumulated higher concentrations of Cd. These findings have revealed that earthworm activities can modify the availability of heavy metals for uptake by plants in contaminated soils. Moreover, the study results show that the ecological context of phytoremediation should be broadened by considering earthworm-plant-soil interaction, which influence both the health of the plant and the uptake of heavy metals by plants.

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“…The cytotoxic effect may not be contributed by a single compound, but is more likely to reflect the cumulative effects of contaminants present in water. Heavy metals, individually or in combination, may exert a strong inhibitory effect on the cell division (Unyayar et al, 2006).…”

Section: Mitotic Indexmentioning

confidence: 99%

Efficacy of Avena Sativa L. And Trifolium Alexandrinum L. Test System for Detecting the Genotoxic and Phytotoxic Potential of Water Pollution

Khelif¹

2016

Appl Ecol Env Res

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Abstract. The Reghaïa Lake (Algiers, Algeria) is contaminated by the untreated industrial effluents discharge of Reghaïa-Rouiba industrial park, via El Biar River. The present report is the first study which addresses the effects of industrial wastewaters rejected into the lake in light of genotoxic and phytotoxic effects, using Avena sativa L. and Trifolium alexandrinum L. test system. The wastewaters were collected seasonally at the mouth of El Biar River and analyzed using chemical analysis and biological assays, including the germination and root elongation inhibition test, mitotic index and micronucleus test. Results showed that samples collected during the summer season (SM) were high contaminated by heavy metals than that of the winter season (WN). Furthermore, the WN samples have relatively low phytotoxicity on A. sativa seed germination and T. alexandrinum root elongation (no EC50 was determined). Genotoxicity of the SM samples is evident in A. sativa by a significant depression of mitotic activity, induction of several chromosomal aberrations and occurrence of micronuclei in T. alexandrinum. All the results indicated that the samples from the El Biar discharging river in Reghaïa Lake exhibited genotoxicity and argue for the possible use of A. sativa and T. alexandrinum plant system for genotoxicity assays.

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Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba

Cited by 225 publications

References 44 publications

Effect of Copper on the Toxicity and Genotoxicity of Cadmium in Duckweed (Lemna Minor L.)

Effect of Copper on the Toxicity and Genotoxicity of Cadmium in Duckweed (Lemna Minor L.)

Earthworms Eisenia fetida affect the uptake of heavy metals by plants Vicia faba and Zea mays in metal-contaminated soils

Efficacy of Avena Sativa L. And Trifolium Alexandrinum L. Test System for Detecting the Genotoxic and Phytotoxic Potential of Water Pollution

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