Cadmium induces apoptosis partly via caspase-9 activation in HL-60 cells

Kondoh, Masuo; Araragi, Saeko; Sato, Katsuya; Higashimoto, Minoru; Takiguchi, Mitsuyoshi; Sato, Masao

doi:10.1016/s0300-483x(01)00536-4

Cited by 171 publications

(104 citation statements)

References 20 publications

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“…Caspases, which are a family of cysteine-dependent aspartatedirected proteases, play important roles in initiating and executing apoptosis. Some studies strongly suggest that caspases play a key role in Cd 2+ -induced cell death (Kim et al, 2000;Li et al, 2000;Kondoh et al, 2002). Previously, we showed that the apoptosis mechanism of Cd 2+ in auditory cells might occur, at least in part, through a caspasedependent pathway .…”

Section: +mentioning

confidence: 88%

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The protective effect of anti-oxidant in cadmium-induced hair cell death

Kim¹,

Myung²,

Jeong³

et al. 2009

Oriental Pharmacy and Experimental Medicine

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SUMMARY Cadmium (Cd +) is a heavy metal and a potent carcinogen implicated in tumor development through occupational and environmental exposure. However, the auditory effect of Cd 2 + is not poorly understood. The purpose of the present study was to investigate whether prevent the ototoxic effects of Cd 2 + by antioxidnat in auditory cell line, HEI-OC1. Rosmarinic acid is a naturally occurring phenolic compound and also known to possess marked antioxidant properties. We showed that antioxidnat inhibited the the cytochrome c release induced by Cd 2 + . In addition, we showed that the regulatory effect of antioxidnat on apoptosis is through the caspase-9 activation, and extracellular signal-regulated kinase activation in auditory cells. These results suggested antioxidnat its therapeutic usefulness, against Cd 2 + induced activation of caspase-9 and ERK.

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Section: +mentioning

confidence: 88%

“…-induced cell death (Kim et al, 2000;Li et al, 2000;Kondoh et al, 2002), whereas other studies suggest that Cd 2+ induced the apoptosis through caspase-independent pathway (Harstad and Klaassen 2002;Shih et al, 2003;Lemarie et al, 2004). This apparently conflicting data suggests that the pathways of Cd…”

Section: +mentioning

confidence: 96%

The protective effect of anti-oxidant in cadmium-induced hair cell death

Kim¹,

Myung²,

Jeong³

et al. 2009

Oriental Pharmacy and Experimental Medicine

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“…DNA Fragmentation Assay ---DNA fragmentation assays were performed as described previously. 14) Briefly, total DNA extracted from cells was electrophoresed on a 1.8% agarose gel, and DNA ladders were visualized by ethidium bromide staining. Immunoblot Analysis ---Immunoblot analysis was carried out as described previously.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblot Analysis ---Immunoblot analysis was carried out as described previously. 14,19) Briefly, cells were lysed in lysis buffer [1% Nonidet P-40, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 1 mM phenylmethylsulfonyl fluoride, 1 mM ethylenediaminetetraacetic acid]. Cell lysates were prepared by sonication, and then the lysates were centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…[6][7][8] Exposure to Cd results in immunotoxicity and injury of the liver and kidneys, and recent studies have indicated that the toxicity of Cd is partly involved in induction of apoptosis. [9][10][11][12] Although induction of apoptosis by Cd is triggered by activation of caspases which are initiators and executors of apoptosis, 8,13,14) association of the stressinduced signaling cascade with Cd-induced apoptosis remains to be proved.…”

Section: Introductionmentioning

confidence: 99%

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Activation of a 36-kDa Myelin Basic Protein Kinase during Cadmium-Induced Apoptosis in Human Leukemia HL-60 Cells

Kondoh

Watanabe

Sato³

2004

JOURNAL OF HEALTH SCIENCE

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Cadmium (Cd) is a well-known modulator of intracellular signal transduction including mitogen-activated protein kinases and protein kinase C. In this study, we investigated activation of a kinase using myelin basic protein (MBP) as a substrate during Cdinduced apoptosis in human leukemia HL-60 cells. To detect a kinase during Cd-induced apoptosis in HL-60 cells, we performed an in-gel kinase assay using MBP as a substrate and found that Cd induced the activation of a kinase with an apparent molecular mass of 36 kDa. The time-course of appearance of DNA ladders induced by Cd was consistent with that of activation of this kinase. The kinetics of activation of p36 MBP kinase was different from that of p38 mitogenactivated protein kinase (p38MAPK). Activation of p36 MBP kinase was also observed with kinetics distinct from that of activation of p38MAPK during mercuric chloride-induced apoptosis. This is the first report on activation of p36 MBP kinase during Cd-or Hg-induced apoptosis.

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Biofunctionalization of Fluorescent Nanoparticles

Murcia

Naumann

2007

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Fluorescent Nanoparticle Probes Dye‐doped Nanoparticles Quantum Dots ( QDs ) Metal Nanoparticles Hybrid Architectures Involving Fluorescent Nanoprobes Metal–Dye Dye‐doped Silica Shells Quantum Dot‐containing Microspheres Bioconjugation of Fluorescent Nanoparticles General Considerations Overview Common Coupling Reactions Bioconjugation of Polymeric Nanoparticles Noncovalent Approaches Covalent Approaches Bioconjugation of Quantum Dots Noncovalent Approaches Covalent Approaches Bioconjugation of Metallic Nanoprobes Noncovalent Approaches Covalent Approaches Design of Biocompatible Coatings General Considerations Overview Colloidal Stability Biocompatible Surfaces Cytotoxicity Nanoparticle‐stabilizing Coatings Low Cytotoxicity Coatings Applications Biosensing Polymeric Sensors Quantum Dot Sensors Metallic Sensors Fluorescent Nanoparticles as Labels in Biological Imaging Dye‐doped Nanoparticles Quantum Dots

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Cadmium induces apoptosis partly via caspase-9 activation in HL-60 cells

Cited by 171 publications

References 20 publications

The protective effect of anti-oxidant in cadmium-induced hair cell death

The protective effect of anti-oxidant in cadmium-induced hair cell death

Activation of a 36-kDa Myelin Basic Protein Kinase during Cadmium-Induced Apoptosis in Human Leukemia HL-60 Cells

Biofunctionalization of Fluorescent Nanoparticles

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