Cadmium pathology in an insect cell line: ultrastructural and biochemical effects

Braeckman, Bart P.; Brys, Kristel; Rzeznik, Urszula; Raes, Hilda

doi:10.1054/tice.1998.0019

Cited by 27 publications

(13 citation statements)

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“…Changes in the cristae ultrastructure during stress conditions leads to breakdown of interactive and communicative means between ETC proteins as well as diminished mitochondrial function and plasticity. Numerous reports using transmission electron microscopy (TEM) evidence deleterious effects of cadmium on mitochondrial cristae, such as reduction in number and shortening, in various animal systems (Asar et al 2004;Braeckman et al 1999;Early et al 1992;Ord et al 1988;Yang et al 2016) and has been correlated with reduced expression of cytochrome c oxidases (COX), essential components of ETC complexes, indicating compromised mitochondrial function (Takaki et al 2004;Toury et al 1985). In view of recent discoveries, future studies are required to elucidate the molecular targeting of cadmium on protein complexes that govern cristae organization.…”

Section: Mitochondrial Membranes: Ultrastructure and Lipid Compositionmentioning

confidence: 99%

Cell organelles as targets of mammalian cadmium toxicity

Lee

Thévenod

2020

Arch Toxicol

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Ever increasing environmental presence of cadmium as a consequence of industrial activities is considered a health hazard and is closely linked to deteriorating global health status. General animal and human cadmium exposure ranges from ingestion of foodstuffs sourced from heavily polluted hotspots and cigarette smoke to widespread contamination of air and water, including cadmium-containing microplastics found in household water. Cadmium is promiscuous in its effects and exerts numerous cellular perturbations based on direct interactions with macromolecules and its capacity to mimic or displace essential physiological ions, such as iron and zinc. Cell organelles use lipid membranes to form complex tightly-regulated, compartmentalized networks with specialized functions, which are fundamental to life. Interorganellar communication is crucial for orchestrating correct cell behavior, such as adaptive stress responses, and can be mediated by the release of signaling molecules, exchange of organelle contents, mechanical force generated through organelle shape changes or direct membrane contact sites. In this review, cadmium effects on organellar structure and function will be critically discussed with particular consideration to disruption of organelle physiology in vertebrates.

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Section: Mitochondrial Membranes: Ultrastructure and Lipid Compositionmentioning

confidence: 99%

Cell organelles as targets of mammalian cadmium toxicity

Lee

Thévenod

2020

Arch Toxicol

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“…The presence of cadmium-related effects was studied on the ultrastructural level in the epidermis and undifferentiated neoblasts of animals exposed to 10 mM CdCl 2 for 1, 2 and 3 weeks. Based on the results of Braeckman et al, (1999) following cadmium-related effects were studied: nuclear chromatin clumping, indentation, filling and dilatation of the perinuclear cisternae, condensation and/or dilatation of mitochondria, the presence of increased amounts of free and membrane bound ribosomes, filling and dilatation of the rough endoplasmic reticulum and an increase of the lysosomal system. However, no significant effects were observed on the ultrastructure of neither the neoblasts nor the epidermis (data not shown).…”

Section: Cadmium Accumulationmentioning

confidence: 99%

Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea

Plusquin

Stevens²,

Belleghem³

et al. 2012

Int. J. Dev. Biol.

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The planarian Schmidtea mediterranea is a well-studied model organism for developmental research, because of its stem cell system. This characteristic also provides a unique opportunity to study stress management and the effect of stress on stem cells. In this study, we characterised the stress signature at different levels of biological organization. The carcinogenic metal cadmium was used as a model chemical stressor. We focused on stem cell activity and its interaction with other known stress parameters. Here, we have found that S. mediterranea is able to cope with high internal levels of cadmium. At endpoints such as size and mobility, cadmium-related stress effects were detected but all of these responses were transient. Correspondingly, cadmium exposure led to an elevated mitotic activity of the neoblasts, at the same time points when the other responses disappeared. At the molecular level, we observed redox-related responses that can be linked with both repair as well as proliferation mechanisms. Together, our results suggest that these animals have a high plasticity. The induction of stem cell activity may underlie this 'restoring' effect, although a carcinogenic outcome after longer exposure times cannot be excluded.

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“…By the same token, HSI indication of soft tissue darkening of hydropsychids in the present study may be an early-warning signal of color change related to metabolic disturbances. Cd is observed to alter normal cell ultrastructure in insects, which is linked to protein-based defense system activation (Braeckman et al 1999a, b). Another explanation may be related to adsorption of Cd on body surfaces.…”

Section: Resultsmentioning

confidence: 99%

Hyperspectral Imaging of Macroinvertebrates—a Pilot Study for Detecting Metal Contamination in Aquatic Ecosystems

Salmelin

Pölönen

Puupponen

et al. 2018

Water Air Soil Pollut

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The applicability of spectral analysis in detection of freshwater metal contamination was assessed by developing and testing a novel hyperspectral imaging (HSI) application for aquatic insect larvae (Trichoptera: Hydropsychidae). Larvae were first exposed to four different cadmium (Cd) concentrations: 0, 1, 10 and 100 μg L⁻ 1 for 96 h. Individual larvae were then preserved in ethanol, inspected with microscopy for the number of anomalies in larval gills, and imaged by hyperspectral camera operating with wavebands between 500 and 850 nm. Three additional larvae from each exposure were analyzed for tissue Cd concentration. Although the larval tissue Cd concentrations correlated positively with actual water concentrations, the toxicity response of larvae i.e. frequency of gill abnormalities did not differ among the Cd concentrations. In contrast, hyperspectral imaging data indicated some concentration-response relationship of larval spectral properties to the Cd exposure, but it was too weak for reliable automatic distinction between exposed and unexposed larvae. In this pilot study a workflow for data processing for a novel application of hyperspectral imaging was developed. Based on the results of this preliminary study, the workflow in the imaging process will be optimized and its potential for detecting metal contamination of aquatic environments reassessed.

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Cadmium pathology in an insect cell line: ultrastructural and biochemical effects

Cited by 27 publications

References 0 publications

Cell organelles as targets of mammalian cadmium toxicity

Cell organelles as targets of mammalian cadmium toxicity

Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea

Hyperspectral Imaging of Macroinvertebrates—a Pilot Study for Detecting Metal Contamination in Aquatic Ecosystems

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