Cadmium retention in rice roots is influenced by cadmium availability, chelation and translocation

Nocito, F.F.; Lancilli, C.; Dendena, Bianca; Lucchini, Giorgio; Sacchi, Gian Attilio

doi:10.1111/j.1365-3040.2011.02299.x

Cited by 195 publications

(132 citation statements)

References 79 publications

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“…Our yeast result is different from two previous studies (Nocito et al, 2011;Satoh-Nagasawa et al, 2012), but consistent with a recent study with HvHMA2, the closest homolog in barley (Hordeum vulgare; Mills et al, 2012). Increased cadmium tolerance by expression of OsHMA2 was very small in the study by Satoh-Nagasawa et al (2012).…”

Section: D-f; Supplementalcontrasting

confidence: 57%

“…3D). Because recent studies reported that OsHMA2 may be implicated in cadmium transport (Nocito et al, 2011;Satoh-Nagasawa et al, 2012;Takahashi et al, 2012), we then also compared cadmium concentration between the mutant lines and wild-type rice. Similar to zinc, the mutant lines contained lower levels of cadmium in the shoots and slightly higher cadmium in the roots compared with the wild-type rice (Supplemental Figs.…”

Section: Mutation Of Oshma2 Resulted In Impaired Delivery Of Zinc To mentioning

confidence: 99%

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Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

et al. 2013

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Developing tissues such as meristems and reproductive organs require high zinc, but the molecular mechanisms of how zinc taken up by the roots is preferentially delivered to these tissues with low transpiration are unknown. Here, we report that rice (Oryza sativa) heavy metal ATPase2 (OsHMA2), a member of P-type ATPases, is involved in preferential delivery of zinc to the developing tissues in rice. OsHMA2 was mainly expressed in the mature zone of the roots at the vegetative stage, but higher expression was also found in the nodes at the reproductive stage. The expression was unaffected by either zinc deficiency or zinc excess. OsHMA2 was localized at the pericycle of the roots and at the phloem of enlarged and diffuse vascular bundles in the nodes. Heterologous expression of OsHMA2 in yeast (Saccharomyces cerevisiae) showed influx transport activity for zinc as well as cadmium. Two independent Tos17 insertion lines showed decreased zinc concentration in the crown root tips, decreased concentration of zinc and cadmium in the upper nodes and reproductive organs compared with wild-type rice. Furthermore, a short-term labeling experiment with 67 Zn showed that the distribution of zinc to the panicle and uppermost node I was decreased, but that, to the lower nodes, was increased in the two mutants. Taken together, OsHMA2 in the nodes plays an important role in preferential distribution of zinc as well as cadmium through the phloem to the developing tissues.

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Section: D-f; Supplementalcontrasting

confidence: 57%

Section: Mutation Of Oshma2 Resulted In Impaired Delivery Of Zinc To mentioning

confidence: 99%

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

et al. 2013

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“…Thus, here, we confirmed that OsHMA3 does not transport Zn. As a whole, the phenotypes of oshma2 OsHMA3-RNAi plants in this study verified the sequential function of OsHMA3 and OsHMA2 in plant; OsHMA3 transports only Cd into vacuoles (Miyadate et al 2011;Ueno et al 2010) and OsHMA2 transports Cd and Zn into the xylem of rice roots (Nocito et al 2011;SatohNagasawa et al 2012;Takahashi et al 2012). Moreover, our results suggest the importance of the complete loss of function of OsHMA2 to minimize Cd translocation from the roots to shoots.…”

Section: Haf1supporting

confidence: 54%

“…On the other hand, insertion mutants resulting in the loss of the function of another heavy metal P-type ATPase in rice, OsHMA2 were used to demonstrate the gene function. OsHMA2 transports Cd and zinc (Zn) into the xylem and is responsible for the translocation of Cd and Zn (Nocito et al 2011;SatohNagasawa et al 2012;Takahashi et al 2012).…”

mentioning

confidence: 99%

Functional relationship heavy metal P-type ATPases (OsHMA 2 and OsHMA3) of rice (Oryza sativa) using RNAi

Satoh-Nagasawa

Mori

Sakurai

et al. 2013

Plant Biotechnology

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Oryza sativa heavy metal P-type ATPase 3 (OsHMA3) plays a major role in the hyperaccumulation of cadmium (Cd) by the rice cultivars 'Cho-Ko-Koku (CKK)' and ' Anjana Dhan.' On the other hand, mutants that accumulate Cd in the shoots at a very low level have suggested the function of several genes such as OsHMA2 (which is neccesary to load Cd into xylem) or O. sativa natural resistance-associated macrophage protein 5 (OsNRAMP5, which regulates the uptake of Cd from soil into the roots). Although many studies have examined metal transporters, few have investigated the function of combination of multiple gene in plant. Herein, first, we used an RNA interference (RNAi) method to down regulate OsHMA3 and recovered plants in which the expression of OsHMA3 was strongly suppressed. Second, crossing of OsHMA3 RNAi plants with oshma2 mutants revealed a functional relationship between OsHMA3 and OsHMA2 in plant. We concluded that a complete loss of function of OsHMA2 is crucial to minimize Cd translocation from the roots to the shoots. The current findings also implied that the activity of OsHMA2 can be accelerated in plant in the roots without the C-terminal region of the protein.

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“…The synthesis of PCs in response to Cd has been reported in a number of studies conducted on various species including a marine diatom (Thalassiosira nordenskioeldii, Wang and Wang, 2011), freshwater green alga (Scenedesmus vacuolatus, Le Faucheur et al, 2005), tobacco cell culture (Nicotiana tabacum, Zitka et al, 2011), bread wheat (Triticum aestivum, Ranieri et al, 2005), rice (Nocito et al, 2011) and broad bean (Vicia faba, Čabala et al, 2011). The fact that no PCs were detected in the roots of lettuce grown in either Cd treatment indicates that PCs were not involved in Cd 2+ chelation and accumulation in lettuce root.…”

Section: Phytochelatinsmentioning

confidence: 96%

Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

Akhter

McGarvey

Macfie

2012

Journal of Plant Physiology

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SummaryCadmium (Cd) is a non-essential trace element and its environmental concentrations are approaching toxic levels, especially in some agricultural soils. Understanding how and where Cd is stored in plants is important for ensuring food safety. In this study, we examined two plant species that differ in the distribution of Cd among roots and leaves. Lettuce and barley were grown in nutrient solution under two conditions: chronic (4 week) exposure to a low, environmentally relevant concentration (1.0 µM) of Cd and acute (1 h) exposure to a high concentration (5.0 mM) of Cd. Seedlings grown in solution containing 1.0 µM CdCl 2 did not show symptoms of toxicity and, at this concentration, 77% of the total Cd was translocated to leaves of lettuce, whereas only 24% of the total Cd was translocated to barley leaves. We tested the hypothesis that differential accumulation of Cd in roots and leaves is related to differential concentrations of phytochelatins (PCs), and its precursor peptides. The amounts of PCs and their precursor peptides in the roots and shoots were measured using HPLC. Each of PC 2-4 was synthesized in the barley root upon chronic exposure to Cd and did not increase further upon acute exposure. In the case of lettuce, no PCs were detected in the root given either Cd treatment.The high amounts of PCs produced in barley root could have contributed to preferential retention of Cd in barley roots.

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Cadmium retention in rice roots is influenced by cadmium availability, chelation and translocation

Abstract: Analysis of rice plants exposed to a broad range of relatively low and environmentally realistic Cd concentrations showed that the root capacity to retain Cd ions rose from 49 to 79%, corresponding to increases in the external Cd

Cited by 195 publications

References 79 publications

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

Preferential Delivery of Zinc to Developing Tissues in Rice Is Mediated by P-Type Heavy Metal ATPase OsHMA2

Functional relationship heavy metal P-type ATPases (OsHMA 2 and OsHMA3) of rice (Oryza sativa) using RNAi

Reduced translocation of cadmium from roots is associated with increased production of phytochelatins and their precursors

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