Cadmium sorption to plasma membrane isolated from barley roots is impeded by copper association onto membranes

Kumakura, Hiroaki; Kudo, Kazuaki; Ambo, Hirokazu; Uemura, Matsuo; Kawai, Soshi

doi:10.1016/j.plantsci.2010.09.008

Cited by 9 publications

(9 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plants, it was previously established that Cd inhibits the photosynthetic rate with toxic Cd effects depending on the applied concentrations, the species and cultivation characteristics, the age of leaves and the phonological development of plants (Lagriffoul et al, 1998;Lux et al, 2004;Khan et al, 2013). The rate of photosynthesis, chlorophyll content, activities of photosystem 1 (ps1) and ps11 declined progressively with increasing concentration of applied Cd (Lux et al, 2004;Dunwei et al, 2009;Kudo et al, 2011). Moreover, human exposure to Cd and Cd containing compounds are known to be carcinogenic, hence can induce kidney dysfunction, renal disease that are characterized by severe tubular combined with glomerular disorders (Friberg, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…When taken up in excess, Cd can disturb almost all physiological processes in plants (Siedlecka and Baszyń-Aski, 1993). It is one of the most toxic metals in plants, active at concentration much lower than those of other heavy metals (Kudo et al, 2011). The metal can affect the overall metabolism through alterations in both the behavior of the key enzymes of important pathways (Shah et al, 2001), and membrane composition and function (Fodor et al, 1995;Madejón et al, 2006), including lowering the control of the cell redox state which ultimately causes oxidative stress (Gratao et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…The metal can affect the overall metabolism through alterations in both the behavior of the key enzymes of important pathways (Shah et al, 2001), and membrane composition and function (Fodor et al, 1995;Madejón et al, 2006), including lowering the control of the cell redox state which ultimately causes oxidative stress (Gratao et al, 2005). Cadmium inhibits the photoactivation of photosystem 2 (PS2) by inhibiting electron transfer (Kudo et al, 2011). Hence, Cd can lead to the generation of reactive oxygen species (ROS) indirectly by production of disturbances in the chloroplasts.…”

Section: Introductionmentioning

confidence: 99%

“…Lipid peroxidation is the destructive oxidative process by ROS or nitrogen radicals of animals and plants fatty acids (Kudo et al, 2011). Thus, these polyunsaturated fatty acids are converted to lipid hydroperoxides (LOOH), rendering damage to biological membrane (Khan et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Agbadah¹,

Nwachukwu²,

Okoh³

2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The effects of ethylenediaminetetra-acetic acid (EDTA) on germination, length of stem, area of leaf, fresh weight, level of lipid per oxidation, alkaline phosphatase, acid phosphatase, super oxide dismutase (SOD) and catalase in the roots of cadmium (Cd) treated maize (Zea mays L) and cowpea (Vigna unguiculata L) seedlings after 7 and 21 days of germination were determined in this study. The results obtained, indicate that at the end of 7 and 21 days of exposure to Cd, percentage germination of the seeds were not significantly different in both control and test soil (p>0.05). Morphological parameters (area of leaf, length of stem and fresh weight) were significantly reduced by Cd after 7 and 21 days. The supplementation of the soil sample with EDTA (0.5 mM or 1.0 mM) reversed the effect of Cd on these parameters as it significantly increased length of stems, area of leaf and plant fresh weight. There was a significant decrease in root acid phosphatase, root alkaline phosphatase, super-oxide dismutase (SOD) and catalase activity in both plant species. Both used doses of EDTA to ameliorate the above biochemical parameters. Increased level of root lipid peroxidation in Cd treated maize and cowpea seedlings was observed after 7 and 21 days of germination. Albeit, the level of lipid peroxidation in the root of Cd treated maize was significantly higher than that of cowpea, an indication that cowpea may be more tolerant than maize to Cd toxicity. The treatment of plant with, concentrations of EDTA (0.5 and 1.0 mM) failed to decrease the Cd induced, but increased the level of root lipid peroxidation. These results indicate that EDTA (0.5 mM and 1.0 mM) could be used for the treatment of Cd toxicity in plants; although, EDTA did not totally protect cowpea seedling from oxidative stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Agbadah¹,

Nwachukwu²,

Okoh³

2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Recently it was suggested and indicated from experiments with plasma membrane preparations obtained from roots of barley (Hordeum vulgare L. cv. Minorimugi) that Cu association to plasma vesicles occurs quicker than Cd and this resulted in hindering of the access of Cd to plasma membrane vesicles [17]. Copper is a microelement that has roles in photosynthesis, respiration, antioxidant activity, cell wall metabolism and hormone perception.…”

Section: Effect Of CD On the Content Of Cumentioning

confidence: 99%

Effect of cadmium stress on barley tissue damage and essential metal transport into plant

et al. 2014

View full text Add to dashboard Cite

Conductometric assessment of electrolyte leakage from spring barley plant organs subjected to higher cadmium concentrations 10-2 – 10-4 mol l-1 revealed serious tissue damage. Therefore plants were grown under controlled environment conditions in a phytotron in Knop´s nutrient solution in two variants – the control variant without cadmium and experimental variant treated during the growth of plants with CdCl2 solution of 10-5 mol l-1. Barley plants were then separated into roots, residual caryopses, leaf bases, and leaves. Lyophilized samples were analyzed for the uptake and redistribution of cadmium, calcium, copper, iron, zinc, and manganese in plant organs by spectrometric methods. Cadmium caused significant decrease of calcium and manganese levels in all analyzed plant parts indicating that cadmium was a distinctive antagonist to intake of these metals. Cadmium treatment also lowered amounts of zinc and iron in roots. An opposite trend was characteristic for Cu, where Cd stress caused significant Cu increase and retention in roots and significant decrease in leaves. The combined usage of the electroanalytical technique - conductometry and spectrometric methods (ICP-OES, AAS) proved to be a useful tool for monitoring of tissue damage and intake and transfer of cadmium and macro- and microelements into barley organs.

show abstract

Isolated cell walls exhibit cation binding properties distinct from those of plant roots

et al. 2014

View full text Add to dashboard Cite

The principal contributor to the cation binding properties of roots is currently considered to be the cell wall or, alternatively, the plasma membrane. The aim of this study was to highlight their respective contributions in the binding properties. Methods Cell walls of a dicotyledon (Solanum lycopersicum L.) and monocotyledon (Triticum aestivum L.) were isolated from roots and their binding properties were compared to those of their respective roots. Cell wall and root binding capacities were evaluated by potentiometric titrations and cation exchange capacity measurements, while their biochemical composition was analyzed by 13C-NMR spectroscopy. Results The lower binding capacity of isolated cell walls compared to roots revealed that cell plasma membranes had a higher binding site density than cell walls. The significant decrease in some NMR signals, i.e. carbonyl C, N alkyl/methoxyl C and alkyl C regions, suggested that carboxyl, amine and phosphate binding sites, borne by proteins and phospholipid plasma membranes, contribute to the binding capacity. Conclusions Cell walls and plasma membranes were found to be jointly involved in root binding properties and their respective contributions seemed vary between plants. (Résumé d'auteur

show abstract

Cadmium sorption to plasma membrane isolated from barley roots is impeded by copper association onto membranes

Cited by 9 publications

References 41 publications

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Biochemical effects of ethylene diamine tetra-acetic acid (EDTA) on cadmium treated maize (Zea mays L.) and cowpea (Vigna unguiculata L.)

Effect of cadmium stress on barley tissue damage and essential metal transport into plant

Isolated cell walls exhibit cation binding properties distinct from those of plant roots

Contact Info

Product

Resources

About