Cadmium Uptake and Bioaccumulation inXenopus laevisEmbryos at Different Developmental Stages

Herkovits, Jorge; Cardellini, Pietro; Pavanati, Cristina; Pérez-Coll, Cristina S.

doi:10.1006/eesa.1997.1586

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…A variety of studies have shown that fi sh, as well as other aquatic organisms, are particularly sensitive to pollutants and heavy metals in the early stages of their life cycle (Herkovits et al, 1998;Hernandez et al, 2006;Linbo et al, 2006;Witeska et al, 1995). However, some studies have shown that fi sh eggs are more resistant than young fi sh to the toxic effects of copper sulfate.…”

Section: Discussionmentioning

confidence: 99%

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

et al. 2011

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Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fi sh. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fi sh. Here, acute toxicity tests and LC50 curves have been generated for zebrafi sh larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentrationdependent manner, resulting in changes in gene expression. Using a transgenic fi sh line that expresses enhanced green fl uorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specifi c stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.

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Section: Discussionmentioning

confidence: 99%

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

et al. 2011

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“…Furthermore, this differential accumulation between embryos and larvae was also observed for R. arenarum exposed to diazinon (Aronzon et al 2014), although it was much less pronounced in the case of diazinon, in which embryo uptake could be B27 times greater than larvae. In contrast, it was seen that both R. arenarum and Xenopus laevis exposed to cadmium at different developmental stages showed an increase in metal uptake at later developmental stages that was thought to be related to the gradual increase in the external surface of the larvae, the development of gills, and the possible transport of the contaminant across the membrane from the surface to inner organs (Herkovits and Pérez-Coll 1996;Herkovits et al 1998).…”

Section: Discussionmentioning

confidence: 89%

Differential Uptake of Endosulfan in the South American Toad Under Sublethal Exposure

Svartz

Marino

Ronco

et al. 2015

Arch Environ Contam Toxicol

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Agroecosystems are usually polluted with a wide variety of contaminants with pesticides being very frequently detected. Endosulfan, an organochlorine pesticide, has been shown to cause both lethal and sublethal effects on aquatic organisms such as amphibians and especially on its early developmental stages. In this context, the aim of this study was to evaluate the uptake of environmentally relevant concentrations of endosulfan and its correlation with differential sensitivity in the early development stages of the common South American toad, Rhinella arenarum. Embryos and larvae were exposed to sublethal concentrations of endosulfan for several periods of exposures. According to the developmental stage at which they were exposed, the uptake rate was different. Bioconcentration factors (BCFs) for embryos significantly decreased with exposure time and concentration (p < 0.05) reaching a BCF of ≤1679 for embryos at 96 h of exposure to 0.001 mg endosulfan L(-1). BCFs for larvae significantly increased with exposure time (p < 0.05) obtaining a maximum of 40 at 504 h. In our previous study, we reported that embryos were less sensitive to the effects of endosulfan than larvae, which is in line with the main tendency of embryos to bioconcentrate endosulfan as observed in this study.

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“…Toxicological and ecotoxicological studies performed in adult amphibians have demonstrated that acute or chronic Cd-exposure results in an accumulation of the metal in various target organs, such as the liver, kidney, gonads and skin (Vasil'eva et al, 1987;Vogiatzis and Loumbourdis, 1997;Othman et al, 2009;Dobrovoljc et al, 2012) at levels proportional to the cadmium concentration in the surrounding environment (Herkovits et al, 1998;James et al, 2004a). Most of the studies on the toxicological impact of Cd in target cells and organs have focused on the kidney, liver and gonads (Loumbourdis and Vogiatzis, 2002;Flament et al, 2003;Sura et al, 2006;Brunt et al, 2011;Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 98%

Short-term cadmium exposure induces stress responses in frog (Pelophylax bergeri) skin organ culture

Simoncelli

Belia

Rosa

et al. 2015

Ecotoxicology and Environmental Safety

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Cadmium Uptake and Bioaccumulation inXenopus laevisEmbryos at Different Developmental Stages

Cited by 13 publications

References 25 publications

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Differential Uptake of Endosulfan in the South American Toad Under Sublethal Exposure

Short-term cadmium exposure induces stress responses in frog (Pelophylax bergeri) skin organ culture

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