Caecal fermentation in rats fed diets containing transgenic potato tubers

Abstract

Caecal fermentation in rats fed diets with 40% autoclaved potato tubers was examined.

Nutritional properties of tubers of conventionally bred and transgenic lines of potato resistant to necrotic strain of Potato virus Y (PVYN).

“…Tubers of transgenic line NTR2.27 (non-translated regions of PVY N genome in antisense orientation) increased the Table 3. Caecal parameters, bacterial enzyme activity and production of short chain fa�y acids (SCFA) in caecum of rats (Juśkiewicz et al, 2004, Zduńczyk et al, 2005b bulk of caecal digesta and the production of SCFA as compared to tubers of the conventional cultivar and the other transgenic clones. Taking into account some deviations, e.g., the higher content of essential amino acids in tubers of line R2.P, higher enzymatic activity in caecal digesta of rats fed a diet with tubers of somaclone Irga, and especially the increased amount of caecal digesta and higher production SCFA in the caecum of rats fed a diet with tubers of line NTR2.27, it seems reasonable to undertake a long-term feeding study to confirm the nutritional properties of tubers of transgenic lines.…”

“…All tubers used in the presented investigations were autoclaved (121°C, 1013 hPa, 15 min), dried (40°C) and ground. Our investigations, involving 3-week feeding experiment on rats, included the following determinations: the chemical composition of tubers and nutritional properties of diets with high (40%) content of tubers (Zduńczyk et al, 2005a), caecal metabolism, serum enzymes and indices of non-specific defence of rats (Zduńczyk et al, 2005b), and shortchain fa�y acids (SCFA) production in the caecum of rats (Juśkiewicz et al, 2004). Table 1 show that the crude protein, starch and fibre content in dry ma�er of potato was comparable in tubers obtained from the conventional cultivar Irga, somaclone Irga and the transgenic lines.…”