Caecomyces sympodialis sp. nov., a new rumen fungus isolated from Bos indicus

Chen, Yo-Chia; Tsai, Sheng-Da; Cheng, Hsueh‐Ling; Chien, Chiu-Yuan; Hu, Chun-Yi; Cheng, Tai-Yi

doi:10.3852/mycologia.99.1.125

Cited by 33 publications

(27 citation statements)

References 15 publications

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“…However, the morphological variability of the genus Caecomyces confused the delimitation of these two bulbous genera. It has been observed that isolates of Caecomyces produced several bulbous holdfasts and several sporangia, especially in old (more than 20 h) cultures (Wubah et al 1991a; Ho and Barr 1995), and even exhibited multisporangiate thalli with sporangia sympodially arising from unbranched sporangiophores (Chen et al 2007). In this study, all of the analyses (except for the parsimony bootstrap analyses of ITS1 and the complete ITS), supported a shared ancestry for Caecomyces and Cyllamyces .…”

Section: Discussionmentioning

confidence: 99%

Phylogeny of anaerobic fungi (phylum Neocallimastigomycota), with contributions from yak in China

Wang

Liu

Groenewald

2016

Antonie van Leeuwenhoek

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The phylum Neocallimastigomycota contains eight genera (about 20 species) of strictly anaerobic fungi. The evolutionary relationships of these genera are uncertain due to insufficient sequence data to infer their phylogenies. Based on morphology and molecular phylogeny, thirteen isolates obtained from yak faeces and rumen digesta in China were assigned to Neocallimastix frontalis (nine isolates), Orpinomyces joyonii (two isolates) and Caecomyces sp. (two isolates), respectively. The phylogenetic relationships of the eight genera were evaluated using complete ITS and partial LSU sequences, compared to the ITS1 region which has been widely used in this phylum in the past. Five monophyletic lineages corresponding to six of the eight genera were statistically supported. Isolates of Caecomyces and Cyllamyces were present in a single lineage and could not be separated properly. Members of Neocallimastigomycota with uniflagellate zoospores represented by Piromyces were polyphyletic. The Piromyces-like genus Oontomyces was consistently closely related to the traditional Anaeromyces, and separated the latter genus into two clades. The phylogenetic position of the Piromyces-like genus Buwchfawromyces remained unresolved. Orpinomyces and Neocallimastix, sharing polyflagellate zoospores, were supported as sister genera in the LSU phylogeny. Apparently ITS, specifically ITS1 alone, is not a good marker to resolve the generic affinities of the studied fungi. The LSU sequences are easier to align and appear to work well to resolve generic relationships. This study provides a comparative phylogenetic revision of Neocallimastigomycota isolates known from culture and sequence data.Electronic supplementary materialThe online version of this article (doi:10.1007/s10482-016-0779-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Phylogeny of anaerobic fungi (phylum Neocallimastigomycota), with contributions from yak in China

Wang

Liu

Groenewald

2016

Antonie van Leeuwenhoek

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“…It could be helpful to re-investigate the morphology of three isolates CR4, W101, and Cy50 used for the determination of Caecomyces sp. (AB334759), C. sympodialis (Chen et al 2007;DQ067604), and C. communis (DQ067605), respectively. Molecular identification, if not co-validated with other independent morphological or physiological markers, is error-prone if reference sequences from type material are lacking and should be handled with caution (Hoffmann et al 2009).…”

Section: Vol 55mentioning

confidence: 99%

Diversity of anaerobic fungi within cow manure determined by ITS1 analysis

et al. 2010

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The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven% of sequences were affiliated with Cyllamyces, 24% with Piromyces, 7% with Anaeromyces, only 2% with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITSbased phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated.

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“…The anaerobic fungus Neocallimastix patriciarum J11 was isolated from the feces of water buffalo as described by Chen et al (8). N. patriciarum J11 was inoculated in rumen fluid contain-ing medium supplemented with 0.5% (wt/vol) barley-derived ␤-glucan (Megazyme International Ireland, Ltd.) for cDNA library construction under anaerobic conditions at 39°C for 72 h. Streptococcus equinus BCRC 12578 was purchased from the Bioresource Collection and Research Centre (Shinchu, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

Catalytic Efficiency Diversification of Duplicate β-1,3-1,4-Glucanases from Neocallimastix patriciarum J11

Hung

Chen

Liu

et al. 2012

Appl Environ Microbiol

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ABSTRACTFour types of β-1,3-1,4 glucanase (β-glucanase, EC 3.2.1.73) genes, designatedbglA13,bglA16,bglA51, andbglM2, were found in the cDNA library ofNeocallimastix patriciarumJ11. All were highly homologous with each other and demonstrated a close phylogenetic relationship with and a similar codon bias toStreptococcus equinus. The presence of expansion and several predicted secondary structures in the 3′ untranslated regions (3′UTRs) ofbglA16andbglM2suggest that these two genes were duplicated recently, whereasbglA13andbglA16, which contain very short 3′UTRs, were replicated earlier. These findings indicate that the β-glucanase genes fromN. patriciarumJ11 may have arisen by horizontal transfer from the bacterium and subsequent duplication in the rumen fungus. β-Glucanase genes ofStreptococcus equinus,Ruminococcus albus7, andN. patriciarumJ11 were cloned and expressed byEscherichia coli. The recombinant β-glucanases cloned fromS. equinus,R. albus7, andN. patriciarumJ11 were endo-acting and had similar substrate specificity, but they demonstrated different properties in other tests. The specific activities and catalytic efficiency of the bacterial β-glucanases were also significantly lower than those of the fungal β-glucanases. Our results also revealed that the activities and some characteristics of enzymes were changed during the horizontal gene transfer event. The specific activities of the fungal β-glucanases ranged from 26,529 to 41,209 U/mg of protein when barley-derived β-glucan was used as the substrate. They also demonstrated similar pH and temperature optima, substrate specificity, substrate affinity, and hydrolysis patterns. Nevertheless, BglA16 and BglM2, two recently duplicated β-glucanases, showed much higherkcatvalues than others. These results support the notion that duplicated β-glucanase genes, namely,bglA16andbglM2, increase the reaction efficiency of β-glucanases and suggest that the catalytic efficiency of β-glucanase is likely to be a criterion determining the evolutionary fate of duplicate forms inN. patriciarumJ11.

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Caecomyces sympodialis sp. nov., a new rumen fungus isolated from Bos indicus

Cited by 33 publications

References 15 publications

Phylogeny of anaerobic fungi (phylum Neocallimastigomycota), with contributions from yak in China

Phylogeny of anaerobic fungi (phylum Neocallimastigomycota), with contributions from yak in China

Diversity of anaerobic fungi within cow manure determined by ITS1 analysis

Catalytic Efficiency Diversification of Duplicate β-1,3-1,4-Glucanases from Neocallimastix patriciarum J11

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