Caenibacillus caldisaponilyticus gen. nov., sp. nov., a thermophilic, spore-forming and phospholipid-degrading bacterium isolated from acidulocompost

Abstract

A thermophilic and phospholipid-degrading bacterium, designated strain B157 T , was isolated from acidulocompost, a garbage compost processed under acidic conditions at moderately high temperature. The organism was Gram-stain-positive, aerobic, spore-forming and rod-shaped. Growth was observed to occur at 40-65 C and pH 4.8-8.1 (optimum growth: 50-60 C, pH 6.2). The strain was catalase-and oxidase-positive. The cell wall contained meso-diaminopimelic acid, alanine, glutamic acid and galactose. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major fatty acids were anteiso-C 17 : 0 and iso-C 17 : 0 . Comparative 16S rRNA gene sequence analysis showed that strain B157 T was related most closely to Tuberibacillus calidus 607 T (94.8 % identity), and the phylogenetic analysis revealed that it belonged to the family Sporolactobacillaceae. The DNA G+C content was determined as 51.8 mol%. In spite of many similarities with the type strains of members of the family Sporolactobacillaceae, genotypic analyses suggest that strain B157 T represents a novel species of a new genus, Caenibacillus caldisaponilyticus gen. nov., sp. nov. The type strain of Caenibacillus caldisaponilyticus is B157 T (=NBRC 111400 T =DSM 101100 T ).

Dynamics of bacterial and archaeal communities during horse bedding and green waste composting

“…Subsequent reductions of species from Young to Middle phases included further loss of thermosensitive species such as Jeotgalicoccus psychrophilus , as well as animal-associated Corynebacterium sp., Kurthia massiliensis and Streptococcus equinus ( Bernard et al, 2010 ; Roux et al, 2012 ; Schlegel et al, 2003 ; Yoon et al, 2003 ), which likely represents reductions in species associated with horse feces present in Litter. The major species increasing in Middle phase included thermophiles common to compost, such as Caenibacillus caldisaponilyticus ( Tsujimoto et al, 2016 ) and unexpected species such as the thermophilic and alkaliphilic Verrucomicrobia Limisphaera ngatamarikiensis ( Carere et al, 2020 ) , but was characterized by significant increases in thermophilic methanogenic archaea, the largest change being observed in Methanothrix thermoacetophila ( Kamagata et al, 1992 ). M. thermoacetophila produces methane under lower oxygen conditions such as those of progressing composting of Middle phase and which likely led to the subsequent increases in moderately thermophilic methanotrophs such Methylocaldum szegediense ( Medvedkova et al, 2009 ).…”

Draft Genome Sequence of Caenibacillus caldisaponilyticus B157 T , a Thermophilic and Phospholipase-Producing Bacterium Isolated from Acidulocompost

“…However, little information was previously reported on novel bacteria from acidulocompost. Caenibacillus caldisaponilyticus B157 T (= NBRC 111400 T = DSM 101100 T ) was isolated as a thermophilic and phospholipid-degrading bacterium from acidulocompost at Oarai Aquarium in Ibaraki, Japan, and thereafter recognized as a novel species of a new genus in the family Sporolactobacillaceae ( 4 ). The organism is Gram positive, aerobic, spore forming, and rod shaped.…”