Cafe Arabica Coffea arabica L.

Aguilar, Miriam; Ortiz, Juan Luis; Mesén, Francisco; Jiménez, L. Diego; Altmann, Fernando

doi:10.1007/978-3-319-79087-9_3

Cited by 6 publications

(20 citation statements)

References 22 publications

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“…The hyperhydricity of the teak in RITA ® decreased considerably (7.0%) by reducing the BA to 0.05 mg•l −1 and the immersion frequency to 1 immersion of 1 minute every two days.Although the number of shoots was low (2.3); there was better use of the shoots since the length was longer (3.7 cm), compared with the treatments where 1 or 2 immersions were used daily. A lower hyperhydricity was also obtained during the germination of somatic embryos of Coffee arabica in RITA ® , when the number of daily immersions was reduced[38] [54]. Very often, high multiplication rates coincide with high rates of hyperhydricity[14] [42][49].…”

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confidence: 99%

Simple Protocol for the Micropropagation of Teak (Tectona grandis Linn.) in Semi-Solid and Liquid Media in RITA&reg; Bioreactors and ex Vitro Rooting

Aguilar¹,

Garita²,

Kim³

et al. 2019

AJPS

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In Latin America the forestry of exotic species such as teak has been increasing in recent decades, due to their advantages in wood quality, rapid growth; and the relative ease of producing clones and their multiplication with respect to native species. Therefore, there is great interest in developing larger-scale propagation strategies that reduce costs and intensive manual labor. Culture in liquid media with temporary immersion and the semi-automation of the system has raised expectations for large-scale micropropagation. We report a protocol for teak, which reuses the primary explants in several culture cycles in semi-solid medium to produce nodal explants for the multiplication phase in temporary immersion bioreactors (RITA ®). The control of factors such as cytokinin concentration, explants density, immersion frequencies and culture duration was analyzed. The number of shoots increased with 0.5 mg•l −1 of BA (6-Benzyladenine), alone or in combination with 0.5 mg•l −1 of Kinetin, with 2 daily immersions of 1 minute each; however, these shoots showed a high degree of hyperhydricity. When 0.05 mg•l −1 of BA was used with 1 immersion of 1 minute every 2 days, the hyperhydricity decreased. Although the number of shoots was lower, they showed good length to be used during multiplication and rooting ex vitro. Our results suggest that teak micropropagation can be simplified in two phases in vitro, the establishment and multiplication; followed by rooting ex vitro and acclimatization. This would imply a reduction in production costs, since most of the multiplication would take place in RITA ® containers.

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confidence: 99%

Simple Protocol for the Micropropagation of Teak (Tectona grandis Linn.) in Semi-Solid and Liquid Media in RITA&reg; Bioreactors and ex Vitro Rooting

Aguilar¹,

Garita²,

Kim³

et al. 2019

AJPS

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“…Since SE was first reported for Robusta coffee ( Staritsky, 1970 ), different coffee species and their varieties ( Söndahl and Sharp, 1977 ; Zamarripa et al., 1991 ; Van Boxtel and Berthouly, 1996 ; De Feria et al., 2003 ; Giridhar et al., 2004 ), clones ( Ducos et al., 2003 ; Santana et al., 2004 ), and hybrids ( Etienne et al., 1997a ; Etienne and Bertrand, 2001 ; Etienne and Bertrand, 2003 ; Etienne et al., 2012 ; Aguilar et al., 2018 ) have been evaluated for their embryogenic competence. Stem ( Staritsky, 1970 ; Nassuth et al., 1980 ) and leaf ( Söndahl and Sharp, 1977 ; Dublin, 1981 ; Yasuda et al., 1985 ; Berthouly and Michaux-Ferriere, 1996 ; Hatanaka et al., 1999 ) explants and parts of zygotic embryos ( Sreenath et al., 1995 ) were evaluated.…”

Section: Se: a Powerful Tool For Clonal Propagation Of Elite Materialsmentioning

confidence: 99%

“…Table 1 presents C. arabica SE divided into five laboratory phases and one greenhouse phase ( Aguilar et al., 2018 ). The culture media are outlined in Table 2 .…”

Section: Se: a Powerful Tool For Clonal Propagation Of Elite Materialsmentioning

confidence: 99%

“…(1985) medium is used. The morphogenetic events that characterize each phase are shown in Figure 1 ( Aguilar et al., 2018 ).…”

Section: Se: a Powerful Tool For Clonal Propagation Of Elite Materialsmentioning

confidence: 99%

“…Technical innovations such as embryogenic cell suspensions (ECS), SE in temporary immersion bioreactors (RITA ® ) ( Berthouly et al., 1995 ; Etienne et al., 1997a ), and direct somatic embryo sowing in the greenhouse have improved the coffee SE technique ( Etienne-Barry et al., 1999 ; Barry-Etienne et al., 2002 ). Recently, complementing SE with classic vegetative propagation techniques such as root cuttings from somaclones has improved hybrid multiplication and lowered production costs ( Aguilar et al., 2018 ; Etienne et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes

Aguilar

Wang²,

Escalona³

et al. 2022

Front. Plant Sci.

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Culture in temporary immersion systems (TIS) is a valuable tool for the semi-automation of high frequency somatic embryogenesis of coffee. This system allows the intermittent exposure of explants to liquid medium in cycles of specific frequency and duration of immersion with renewal of the culture atmosphere in each cycle. TIS have revolutionized somatic embryogenesis of coffee plants as an alternative for scaling up and reducing costs associated with labor-intensive solid media culture. In Central America, somatic embryogenesis is employed on a commercial scale to produce F1 Coffea arabica hybrids. In Asia and Africa, somatic embryogenesis is used for the multiplication of selected genotypes of C. arabica and C.canephora. Somatic embryogenesis of coffee plants is considered a model system for woody species due to its biological versatility and low frequency of somaclonal variation. Nevertheless, the success of somatic embryogenesis for mass propagation of coffee plants depends on the development, optimization, and transfer of complementary technologies. Temporary immersion using the RITA® bioreactor is, so far, the best complementary tool for somatic embryogenesis of Arabica coffee for a single recipient with simple changes in liquid media. Likewise, high volume bioreactors, such as 10-L glass BIT® and 10-L flexible disposable plastic bags, have been successfully used for somatic embryogenesis of other coffee species. These bioreactors allow the manipulation of thousands of embryos under semi-automated conditions. The protocols, advantages, and benefits of this technology have been well documented for organogenesis and somatic embryogenesis pathways. However, adaptation in commercial laboratories requires technical and logistical adjustments based on the biological response of the cultures as well as the costs of implementation and production. This review presents the historical and present background of TIS and its commercial application and, in particular, pertinent information regarding temporary immersion culture for C. arabica somatic embryogenesis. The main limitations of this technology, such as hyperhydricity, asynchrony, and developmental abnormalities, are examined, and a critical analysis of current knowledge regarding physiological, biochemical, and molecular aspects of the plant response to temporary immersion is offered. Further, perspectives are provided for understanding and solving the morpho-physiological problems associated with temporary immersion culture of coffee plants.Systematic Review Registration:

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Protocolo sencillo y eficiente para la extracción de ADN a partir de semillas de cafeto (Coffea canephora Pierre)

Castilla Valdés,

Soto Pérez,

González Vega

2024

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El primer paso para realizar cualquier tipo de estudio genético es contar con un protocolo para el aislamiento de ADN. Aunque el material vegetal más utilizado para ello es el tejido foliar, cuando no existe disponibilidad, las semillas constituyen otra opción y además, durante su traslado del campo al laboratorio no se produce degradación del ADN ni se necesita nitrógeno líquido para su conservación. Sin embargo, la extracción de ADN a partir de semillas de algunas especies como el cafeto, resulta complicado debido a su dureza y contenido de compuestos fitoquímicos. En este trabajo nos propusimos optimizar un protocolo de extracción de ADN de semillas de cafeto (C. canephora) para ser utilizado en análisis moleculares. Se utilizó el método CTAB como control y tres variantes de este. La calidad del ADN se comprobó en geles de agarosa y su concentración fue determinada en un nano-espectrofotómetro. Se realizó la amplificación del ADN por PCR con un cebador de RAPD, y su chequeo en electroforesis. Se logró obtener ADN libre de grasas y polisacáridos, con suficiente calidad para ser utilizado en PCR, como se demostró por la observación en gel, los valores de concentración y relaciones de absorbancia, y la obtención de las bandas que produjo el cebador. Este protocolo permite el acceso a la caracterización genética del cafeto, esencial para la conservación de su diversidad y para el mejoramiento genético. Keywords: ADN, cafeto, extracción, genética, mejoramiento, semillas

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Cafe Arabica Coffea arabica L.

Cited by 6 publications

References 22 publications

Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>&reg; </sup> Bioreactors and <i>ex Vitro</i> Rooting

Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>&reg; </sup> Bioreactors and <i>ex Vitro</i> Rooting

Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes

Protocolo sencillo y eficiente para la extracción de ADN a partir de semillas de cafeto (Coffea canephora Pierre)

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Cafe Arabica Coffea arabica L.

Cited by 6 publications

References 22 publications

Simple Protocol for the Micropropagation of Teak (&lt;i&gt;Tectona grandis&lt;/i&gt; Linn.) in Semi-Solid and Liquid Media in RITA&lt;sup&gt;&amp;reg; &lt;/sup&gt; Bioreactors and &lt;i&gt;ex Vitro&lt;/i&gt; Rooting

Simple Protocol for the Micropropagation of Teak (&lt;i&gt;Tectona grandis&lt;/i&gt; Linn.) in Semi-Solid and Liquid Media in RITA&lt;sup&gt;&amp;reg; &lt;/sup&gt; Bioreactors and &lt;i&gt;ex Vitro&lt;/i&gt; Rooting

Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes

Protocolo sencillo y eficiente para la extracción de ADN a partir de semillas de cafeto (Coffea canephora Pierre)

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Product

Resources

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Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>® </sup> Bioreactors and <i>ex Vitro</i> Rooting

Simple Protocol for the Micropropagation of Teak (<i>Tectona grandis</i> Linn.) in Semi-Solid and Liquid Media in RITA<sup>® </sup> Bioreactors and <i>ex Vitro</i> Rooting