Caffeic acid binds to the minor groove of calf thymus DNA: A multi-spectroscopic, thermodynamics and molecular modelling study

Sarwar, Tarique; Ishqi, Hassan Mubarak; Rehman, Sufia; Husain, Mohammed Amir; Rahman, Yusra; Tabish, Mohammad

doi:10.1016/j.ijbiomac.2017.02.014

Cited by 90 publications

(34 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results, shown in Tables and , strongly exhibited that caffeic acid interacts with DNA. This occurrence of this interaction described as the binding of caffeic acid to the minor groove of calf thymus DNA in the literature . Decrease at oxidation signal of IDR after interaction with DNA in the absence and presence of caffeic acid was calculated as 69 % and 46 % respectively.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Öndeş

Muti

2020

Electroanalysis

View full text Add to dashboard Cite

Now it is well known, antioxidant may affect the interaction of anticancer drugs and DNA. This study aims to investigate the interaction between Idarubicin and DNA and the effect of caffeic acid on this interaction. Disposable, inexpensive, easy handle electrodes were used in this study to investigate the interaction of idarubicin and DNA electrochemically. Idarubicin (IDR) is an anthracyline antitumor antibiotic, which used against one or more types of leukemia. Electrochemical behaviour of IDR was investigated by using cyclic voltammetry. The interaction between anticancer drug, IDR and calf thymus double‐stranded DNA (ctdsDNA) was investigated by using differential pulse voltammetry (DPV) technique in the absence and presence of caffeic acid.

show abstract

Section: Resultsmentioning

confidence: 99%

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Öndeş

Muti

2020

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…In order to evaluate the interaction between ligand, its complexes and FS‐DNA, the binding constant (K b ) and the binding stoichiometry (n) were determined using following equation:

log \frac{S_{0} - normalS}{S} = log K_{b} + nlog ([], Q)

…”

Section: Resultsmentioning

confidence: 99%

“…However, after binding to DNA due to intercalation, fluorescence intensity of EB is considerably increased. Like EB, if compounds intercalates into DNA, it reduces the availability of binding sites to EB, and lead to a significant decrease in the fluorescence intensity of EB‐DNA system . Figure shows the effect of addition of the as‐made compounds on the fluorescence intensity of EB bound to FS‐DNA.…”

Section: Resultsmentioning

confidence: 99%

New transition metal complexes of 9,10‐phenanthrenequinone p‐toluyl hydrazone Schiff base: Synthesis, spectroscopy, DNA and HSA interactions, antimicrobial, DFT and docking studies

Moradinia

Mansournia

Aramesh‐Boroujeni

et al. 2019

Applied Organom Chemis

View full text Add to dashboard Cite

The new complexes of Cu (II) and Ni (II) of a tridentate Schiff base ligand derived from 9,10‐phenanthrenequinone and p‐toluic hydrazide have been synthesized and characterized by elemental analysis, electrical conductometry, FT‐IR, Mass, NMR and UV–Vis. The DFT calculations were carried out at B3LYP/6‐31G*(d) level for the determination of the optimized structure of the ligand and its complexes. The as‐synthesized compounds were screened for their antimicrobial activity. Also, their binding behavior with fish salmon‐DNA (FS‐DNA) and human serum albumin (HSA) were studied by different kinds of spectroscopic and molecular modeling techniques. The fluorescence data at different temperatures were applied in order to estimate the thermodynamics parameters of interactions of ligand and its complexes with DNA and HSA. The results showed that the as‐made compounds could bind to FS‐DNA and HSA via the groove binding as the major binding mode. According to molecular docking calculation and competitive binding experiments, these compounds bind to the minor groove of DNA and hydrophobic residues located in the subdomain IB of HSA. In addition, the molecular docking results kept in good consistence with experimental data.

show abstract

“…The interaction and binding mode of a small molecule with DNA can also be studied by measuring the viscosity of DNA solution in the absence and presence of interacting molecules . It is well known that any molecules that intercalates with the DNA helix causes separation of base pairs and hence increases the overall length of DNA . This causes increase in the viscosity of DNA solution.…”

Section: Resultsmentioning

confidence: 99%

Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Qumaizi

Anwer

Ahmad

et al. 2018

J of Molecular Recognition

View full text Add to dashboard Cite

Pioglitazone is an antidiabetic drug used to treat type 2 diabetes mellitus. Interaction of Pioglitazone with calf thymus DNA was investigated using multispectroscopic techniques and molecular docking study. Quenching and binding constant was calculated at 3 different temperatures. The binding constant of Pioglitazone with calf thymus DNA was calculated to be 6.49 × 10 M at 293 K. The quenching mechanism was found to be a static process, and thermodynamic parameters revealed van der Waals interactions and hydrogen bonds to be the major force working in Pioglitazone-DNA interaction. Pioglitazone follows the nonintercalative mode of binding and was involved in complex formation with DNA through minor groove binding and electrostatic interactions. Experiments like KI quenching studies, dye displacement assays, Circular Dichroism (CD) spectroscopy, DNA melting study, and viscosity measurements studies supported the nonintercalative mode of binding. This was further corroborated by molecular docking studies.

show abstract

Caffeic acid binds to the minor groove of calf thymus DNA: A multi-spectroscopic, thermodynamics and molecular modelling study

Cited by 90 publications

References 37 publications

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

New transition metal complexes of 9,10‐phenanthrenequinone p‐toluyl hydrazone Schiff base: Synthesis, spectroscopy, DNA and HSA interactions, antimicrobial, DFT and docking studies

Study on the interaction of antidiabetic drug Pioglitazone with calf thymus DNA using spectroscopic techniques

Contact Info

Product

Resources

About