Caffeic Acid Genotoxicity: Correlation of the <i>Pig‐a</i> Assay with Regulatory Genetic Toxicology <i>In Vivo</i> Endpoints

Bhalli, Javed A.; Neft, Robin E.; Noteboom, Jessica; Tebbe, Cameron C.; Chan, Michelle; Kuhn, Kylie; Pearce, G. S.; Jordan, Liam; Beevers, Carol

doi:10.1002/em.22333

Cited by 8 publications

(4 citation statements)

References 19 publications

(32 reference statements)

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“…For example, Bhalli, et al evaluated the genotoxic potential of caffeic acid in vivo through the micronucleus assay and the comet assay. Results showed that, through the MN assay, caffeic acid generated a genotoxic effect by increasing the number of micronucleated cells; however, the comet assay did not demonstrate a significant increase in the number of cells with DNA damage [ 47 ]. C. oxyacantha leaves contain vitexin, isovitexin, and orientin.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Aguilera-Rodríguez

Zamora‐Pérez

Galván-Moreno

et al. 2021

Plants

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Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Aguilera-Rodríguez

Zamora‐Pérez

Galván-Moreno

et al. 2021

Plants

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“…However, this phenomenon was not reported in recent studies. Dose of up to 2,000 mg/kg/day CA on rats did not result in gene mutations in Pig‐a assay and blood or liver DNA damage (Bhalli et al., 2019). Similarly, Amano et al.…”

Section: Potential Safety Concerns From Hca Intakementioning

confidence: 99%

Hydroxycinnamic acids on gut microbiota and health

Leonard

Zhang

Ying

et al. 2020

Comp Rev Food Sci Food Safe

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Hydroxycinnamic acids (HCAs) are a major class of phenolic acids with the characteristic phenylpropanoid C6‐C3 backbone. Its typically conjugated status with plant cell wall components and liberation by limited enzymes might be the reason for its neglect by researchers compared to flavonoid‐type polyphenols. The polyphenol–gut microbiota interactions and their impact on human health have captured the interest of researchers recently. In addition, there has been a significant progress over the past few years in understanding the gut microbiota‐modulating effect of HCA using animal model studies. This review discusses the metabolism of HCA in the digestive tract, HCA–gut microbiota interactions, and its link to colorectal cancer, inflammatory bowel diseases, mental‐cognitive impairments, nonalcoholic liver disease, and obesity. The effects of food matrix and processing technologies on HCA bioavailability and HCA–gut microbiota interactions, and HCA safety concerns are also featured in this review. This paper has provided an in‐depth insight on HCA–gut microbiota relationship and identified the current literature gaps for future research.

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“…On the other hand, caffeic acid (2.46%) which is one of the major compounds of TEE from A. hypogaea, showed absence of genotoxicity in other researches. Bhalli et al (2019) demonstrated that 1000 mg/kg b.w. of caffeic acid was not genotoxic using micronuclei test and comet assay.…”

Section: Identification Of the Tegument And Seed Of Peanut Compoundsmentioning

confidence: 99%

Evaluation of cellular safety and the chemical composition of the peanut (Arachis hypogaea L.) ethanolic extracts

Candela

Giordano

Quiroga

et al. 2020

Heliyon

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Arachis hypogaea L. (Leguminosae) is distributed in tropical and subtropical areas. Peanut has high nutritional and commercial value. Scientific research showed that peanut has biological properties such as anticancer, antioxidant, antiinflammatory. However, it is necessary to know if consumption of peanut, either as food or as a phytopharmaceutical implies a health risk. The aim was to evaluate cytotoxicity and genotoxicity of ethanolic extracts from A. hypogaea . Also, chemical characterization of these extracts was performed. Cytotoxicity was evaluated by MTT and Neutral Red Uptake (NRU) assays on Vero cells. Genotoxicity was studied by Micronuclei and comet assays on Balb/C mice. Qualitative and quantitative chemical analysis of extracts were performed. Results showed that extracts have low cytotoxicity. Tegument ethanolic extract (TEE) and Seed ethanolic extract (SEE) were not genotoxic. The treatments with TEE at 250 mg/kg and SEE at 2000 mg/kg revealed (highest concentrations evaluated) some toxicity on blood marrow cells of mice. Chemical characterization indicated that TEE had 74.33 ± 1.10 mg GAE/g of dried extract and SEE had 15.05 ± 0.06 mg GAE/g of dried extract of total phenolic content. Also, proanthocyanidins (O.D. at 550 nm 1.39 ± 0.15) and caffeic acid (2.46%) were identified in TEE. While, linoleic acid (58.84%) oleic acid (11.31%) and palmitic acid (8.37%) were major compounds of SEE. In conclusion, peanut consumption is safe at concentrations recommended for healthy uses, such as nutrition, and phytomedicine.

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Caffeic Acid Genotoxicity: Correlation of the Pig‐a Assay with Regulatory Genetic Toxicology In Vivo Endpoints

Cited by 8 publications

References 19 publications

Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Hydroxycinnamic acids on gut microbiota and health

Evaluation of cellular safety and the chemical composition of the peanut (Arachis hypogaea L.) ethanolic extracts

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