Caffeic acid phenethyl ester (CAPE) prevents transformation of human cells by arsenite (As) and suppresses growth of As-transformed cells

Yang, Chengfeng; Wu, Jing; Zhang, Ronghe; Zhang, Ping; Eckard, Jonathan; Yusuf, Rita; Huang, Xi; Rossman, Toby G.; Frenkel, Krystyna

doi:10.1016/j.tox.2005.05.011

Cited by 35 publications

(24 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, the ferritin levels were much more significantly depleted by As in cytoplasm than in nuclei, suggesting that the defense system against oxidative stress in cytoplasm is impaired by a long-term low-dose As treatment. CAPE, a naturally occurring antioxidant, which was previously shown to prevent As-mediated HOS cell transformation [14], also partially prevented As-mediated ferritin depletion in cytoplasm and completely restored ferritin levels in nuclei. In the absence of As, CAPE had no effect on ferritin levels in HOS cells as compared to the untreated control cells.…”

Section: Decreased Irp-binding Activity In As-8w-hos Cellsmentioning

confidence: 84%

“…Control parental HOS cells were cultured in α-MEM medium containing 5 or 10% FBS. Two types of As-treated cells were generated by incubating cells with a low dose of arsenite (0.1 µM), as previously described [13,14]. Cells designated here as As-8w-HOS were cells that were chronically exposed to NaAsO 2 for 8 weeks.…”

Section: Cell Culturementioning

confidence: 99%

“…As previously described, HOS cell transformation occurred only after 8 weeks of low-dose arsenite treatment [13,14]. Ferritin levels in the parental HOS cells were measured as a function of time of exposure to 0.1 µM arsenite.…”

Section: Changes In Ferritin Levels During the Process Of As-mediatedmentioning

confidence: 99%

“…Other studies show that arsenite is a complete transplacental carcinogen in mice [6], while dimethylarsinic acid, a major metabolite of arsenic in most mammals, including humans, causes bladder cancer in F344 rats [7]. In contrast to its weak mutagenicity, arsenite induces cell transformation of various types of cells to a more malignant phenotype, such as Syrian hamster embryo cells [8], mouse embryo fibroblasts [9], mouse epidermal JB6 C141 cells [10], rat liver-derived TRL 1215 cells [11], normal human immortalized prostate epithelium RWPE-1 cells [12], as well as human osteogenic sarcoma (HOS) cells [13,14]. Many mechanisms involving arsenic-mediated cell transformation have been proposed.…”

Section: Introductionmentioning

confidence: 99%

“…Using a previously developed model of a low-dose and long-term exposure to arsenite [14], we obtained transformed human osteogenic sarcoma (As-T-HOS) cells, which have exhibited anchorage independence [13]. We found that arsenite can affect iron homeostasis through an abnormal iron regulation mechanism.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Altered iron homeostasis involvement in arsenite-mediated cell transformation

Eckard

Chen

et al. 2006

Free Radical Biology and Medicine

Self Cite

View full text Add to dashboard Cite

Chronic exposure to low doses of arsenite causes transformation of human osteogenic sarcoma (HOS) cells. Although oxidative stress is considered important in arsenite-induced cell transformation, the molecular and cellular mechanisms by which arsenite transforms human cells are still unknown. In the present study, we investigated whether altered iron homeostasis, known to affect cellular oxidative stress, can contribute to the arsenite-mediated cell transformation. Using arsenite-induced HOS cell transformation as a model, it was found that total iron levels are significantly higher in transformed HOS cells in comparison to parental control HOS cells. Under normal iron metabolism conditions, iron homeostasis is tightly controlled by inverse regulation of ferritin and transferrin receptor (TfR) through iron regulatory proteins (IRP). Increased iron levels in arsenite transformed cells should theoretically lead to higher ferritin and lower TfR in these cells than in controls. However, the results showed that both ferritin and TfR are decreased, apparently through two different mechanisms. A lower ferritin level in cytoplasm was due to the decreased mRNA in the arsenitetransformed HOS cells, while the decline in TfR was due to a lowered IRP-binding activity. By challenging cells with iron, it was further established that arsenite-transformed HOS cells are less responsive to iron treatment than control HOS cells, which allows accumulation of iron in the transformed cells, as exemplified by significantly lower ferritin induction. On the other hand, caffeic acid phenethyl ester (CAPE), an antioxidant previously shown to suppress As-mediated cell transformation, prevents As-mediated ferritin depletion. In conclusion, our results suggest that altered iron homeostasis contributes to arsenite-induced oxidative stress and, thus, may be involved in arsenite-mediated cell transformation.

show abstract

Section: Decreased Irp-binding Activity In As-8w-hos Cellsmentioning

confidence: 84%

Section: Cell Culturementioning

confidence: 99%

Section: Changes In Ferritin Levels During the Process Of As-mediatedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Altered iron homeostasis involvement in arsenite-mediated cell transformation

Eckard

Chen

et al. 2006

Free Radical Biology and Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

Protective role of olive oil extract of propolis on short and long‐term administration of tamoxifen in rats

Silici,

Kaynar,

Okan

et al. 2023

Environmental Toxicology

View full text Add to dashboard Cite

Tamoxifen (TAM) is an antiestrogenic agent used for adjuvant treatment in estrogen receptor‐positive breast cancers in the pre/post‐menopausal period. This study, it was aimed to determine the effect of olive oil extract of propolis (OEP) on short and long‐term administration of TAM in rats. Wistar albino rats were divided into groups with eight animals in each. Groups: control, OEP, TAM, and OEP + TAM. At the end of the experiment, oxidative stress tests were performed with Enzyme‐Linked ImmunoSorbent Assay (ELISA) on blood and tissue samples (liver, kidney, and ovary) taken from rats. After single‐dose TAM administration, there was a significant increase in red blood cell, hematocrit, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels compared to the control group, a decrease in low‐density lipoprotein (LDL) value, a significant increase in liver enzymes and fasting glucose values was detected compared with the control and propolis groups. A normalizing effect was observed in the group given OEP and TAM combined. The increase in Malondialdehyde (MDA) and the decrease in enzyme activities in tissues are also noteworthy. Propolis application reduced the tissue damage caused by TAM. In addition, improved cytokine levels, which increased with TAM administration. It has been concluded that OEP can be given in supportive treatment, as it improves hematological and antioxidant parameters in TAM treatment.

show abstract

Inhibition of proliferation and induction of apoptosis by EGCG in human osteogenic sarcoma (HOS) cells

Han

Kim

2006

Arch Pharm Res

View full text Add to dashboard Cite

EGCG [(-)-epigallocatechin-3-gallate], a major component of green tea has been considered as a major antioxidant constituent. In addition to having been considered for cancer treatment as a chemopreventive and chemotherapeutic agent, EGCG has recently been attributed an anti-proliferative effect. We re-examined the latter finding in this study and added specific focus on the ability of EGCG to induce apoptosis in human osteogenic sarcoma (HOS) cells. Antiproliferative action of EGCG (IC50 = 35.3 +/- 6.0 microg/mL) appeared to be linked to apoptotic cell death based on morphological changes, chromosomal DNA degradation, and an increase in the sub-G1 apoptotic cell population. Treatment of HOS cells with EGCG gradually activated caspase-3, an established inducer of apoptotic cell death.

show abstract

Caffeic acid phenethyl ester (CAPE) prevents transformation of human cells by arsenite (As) and suppresses growth of As-transformed cells

Cited by 35 publications

References 61 publications

Altered iron homeostasis involvement in arsenite-mediated cell transformation

Altered iron homeostasis involvement in arsenite-mediated cell transformation

Protective role of olive oil extract of propolis on short and long‐term administration of tamoxifen in rats

Inhibition of proliferation and induction of apoptosis by EGCG in human osteogenic sarcoma (HOS) cells

Contact Info

Product

Resources

About