Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes

Kleczka, Anna; Kubina, Robert; Dzik, Radosław; Jasik, Krzysztof; Stojko, Jerzy; Cholewa, Krzysztof; Kabała‐Dzik, Agata

doi:10.3390/molecules25153514

Cited by 24 publications

(22 citation statements)

References 41 publications

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“…Although many proteins can regulate apoptosis through cascade reactions, the Bax/Bcl-2 ratio plays a vital role in regulating apoptosis [31]. Caffeic acid phenethyl ester can control the balance between Bax/Bcl-2 and induce the apoptosis of the ovarian cancer OV7 cells to play an antitumor role [32]. Gambogic acid [30,33] and gallic acid [29] can upregulate the Bax protein expression and downregulate the Bcl-2 protein expression, inducing tumor cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Zhao

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Sinapic acid (Sa) is a small-molecule phenolic acid compound predominant in fruits, vegetables, and grains. This study investigated the antitumor effects of cisplatin (DDP) combined with Sa (Sa/DDP) on the hepatic cancer cells (HCC), HepG2 and SMMC-7721. The HepG2 and SMMC-7721 cells were treated with Sa or Sa/DDP, and the cell proliferation and cell cycle were detected using the MTT assay. The cell migration was detected using the transwell and scratch assays, while apoptosis and autophagy were detected using Hoechst, MDC, and Annexin V-FITC/PI staining. The protein expression was quantitated using the western blot. Sa/DDP was found to not only inhibit cancer cell proliferation and migration but also induce cell apoptosis. Simultaneously, the Sa/DDP combination was found to activate autophagy, and the HCQ autophagy inhibitor enhanced the apoptosis in the Sa/DDP-induced liver cancer cells. The combined use of Sa and DDP makes it an attractive adjuvant therapy strategy for tumors, establishing the prospect of phenolic acid compounds for the adjuvant treatment of hepatocellular carcinoma.

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Section: Discussionmentioning

confidence: 99%

Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Zhao

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…So far, many studies demonstrated that CA and its derivatives (among numerous other potential therapeutic properties) are compounds with potent anticancer effects. In vitro experiments have revealed their cytotoxic properties inter alia on breast [ 64 , 65 ], ovarian [ 66 , 67 ], colon [ 68 , 69 , 70 , 71 ], hepatoma [ 72 , 73 , 74 ], melanoma [ 75 , 76 , 77 ], prostate [ 78 ] and pancreatic [ 30 , 31 , 79 ] cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the In Vitro Cytotoxic Activity of Caffeic Acid Derivatives and Liposomal Formulation against Pancreatic Cancer Cell Lines

et al. 2020

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Pancreatic cancer belongs to the most aggressive group of cancers, with very poor prognosis. Therefore, there is an important need to find more potent drugs that could deliver an improved therapeutic approach. In the current study we searched for selective and effective caffeic acid derivatives. For this purpose, we analyzed twelve compounds and evaluated their in vitro cytotoxic activity against two human pancreatic cancer cell lines, along with a control, normal fibroblast cell line, by the classic MTT assay. Six out of twelve tested caffeic acid derivatives showed a desirable effect. To improve the therapeutic efficacy of such active compounds, we developed a formulation where caffeic acid derivative (7) was encapsulated into liposomes composed of soybean phosphatidylcholine and DSPE-PEG2000. Subsequently, we analyzed the properties of this formulation in terms of basic physical parameters (such as size, zeta potential, stability at 4 °C and morphology), hemolytic and cytotoxic activity and cellular uptake. Overall, the liposomal formulation was found to be stable, non-hemolytic and had activity against pancreatic cancer cells (IC50 19.44 µM and 24.3 µM, towards AsPC1 and BxPC3 cells, respectively) with less toxicity against normal fibroblasts. This could represent a promising alternative to currently available treatment options.

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“…They observed a remarkable decrease in the cell viability, migration, and invasion of SKOV-3 accompanied by an obstructed Ki67 and PCNA expression, nuclear translocation of p65, inhibition of IκB phosphorylation, and NF-κB p65 DNA binding activity in the CAPE treated cells [ 137 ]. In another study, Kleczka et al [ 138 ] reported significant cytotoxicity, decreased lysosomal activity, and the total synthesis of cellular proteins in CAPE-treated OV7 serum ovarian cancer cells. Moreover, this study also reported a CAPE-induced apoptosis via dysregulation of Bax/Bcl-2 balance [ 138 ].…”

Section: Phenolic Acids Involved In the Regulation Of Molecular Targetsmentioning

confidence: 99%

“…In another study, Kleczka et al [ 138 ] reported significant cytotoxicity, decreased lysosomal activity, and the total synthesis of cellular proteins in CAPE-treated OV7 serum ovarian cancer cells. Moreover, this study also reported a CAPE-induced apoptosis via dysregulation of Bax/Bcl-2 balance [ 138 ].…”

Section: Phenolic Acids Involved In the Regulation Of Molecular Targetsmentioning

confidence: 99%

Phenolic Acids-Mediated Regulation of Molecular Targets in Ovarian Cancer: Current Understanding and Future Perspectives

et al. 2023

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Cancer is a global health concern with a dynamic rise in occurrence and one of the leading causes of mortality worldwide. Among different types of cancer, ovarian cancer (OC) is the seventh most diagnosed malignant tumor, while among the gynecological malignancies, it ranks third after cervical and uterine cancer and sadly bears the highest mortality and worst prognosis. First-line treatments have included a variety of cytotoxic and synthetic chemotherapeutic medicines, but they have not been particularly effective in extending OC patients’ lives and are associated with side effects, recurrence risk, and drug resistance. Hence, a shift from synthetic to phytochemical-based agents is gaining popularity, and researchers are looking into alternative, cost-effective, and safer chemotherapeutic strategies. Lately, studies on the effectiveness of phenolic acids in ovarian cancer have sparked the scientific community’s interest because of their high bioavailability, safety profile, lesser side effects, and cost-effectiveness. Yet this is a road less explored and critically analyzed and lacks the credibility of the novel findings. Phenolic acids are a significant class of phytochemicals usually considered in the nonflavonoid category. The current review focused on the anticancer potential of phenolic acids with a special emphasis on chemoprevention and treatment of OC. We tried to summarize results from experimental, epidemiological, and clinical studies unraveling the benefits of various phenolic acids (hydroxybenzoic acid and hydroxycinnamic acid) in chemoprevention and as anticancer agents of clinical significance.

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Caffeic Acid Phenethyl Ester (CAPE) Induced Apoptosis in Serous Ovarian Cancer OV7 Cells by Deregulation of BCL2/BAX Genes

Cited by 24 publications

References 41 publications

Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Effects of Sinapic Acid Combined with Cisplatin on the Apoptosis and Autophagy of the Hepatoma Cells HepG2 and SMMC-7721

Evaluation of the In Vitro Cytotoxic Activity of Caffeic Acid Derivatives and Liposomal Formulation against Pancreatic Cancer Cell Lines

Phenolic Acids-Mediated Regulation of Molecular Targets in Ovarian Cancer: Current Understanding and Future Perspectives

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