Caffeinated beverages contribute to a more efficient inflammatory response: Evidence from human and earthworm immune cells

Alves, Audrei de Oliveira; Weis, Grazielle Castagna Cezimbra; Unfer, Taís Cristina; Assmann, Charles Elias; Barbisan, Fernanda; Azzolin, Verônica Farina; Chitolina, Bruna; Duarte, Thiago; Ribeiro-Filho, Euler Esteves; Boligon, Alexandra Augusti; Vélez-Martin, Eduardo; Palma, Taís Vidal; Andrade, Cínthia Melazzo de; Cruz, Ivana Beatrice Mânica da

doi:10.1016/j.fct.2019.110809

Cited by 16 publications

(12 citation statements)

References 42 publications

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“…Problems in the formation of NETs and also of brown bodies (BBs) which are structures formed by coelomocytes, NETs, microorganisms, and/or undesirable substances have been described in earthworms acutely exposed to Rotenone [9]. On contrary, previous studies, such as performed by Alves et al [18] showed that antioxidant and antiinflammatory caffeinated extracts increased the efficiency of ex vivo NET production in the presence of dead yeasts. As the frequency of amoebocytes does not decrease significantly in exposure to rotenone on 7th and 14th when it compared to itself, it is possible to infer that this molecule produces some level of inhibition in the formation of NETs which could mean a decrease in the immunocompetence of earthworms.…”

Section: Discussionmentioning

confidence: 99%

Superoxide-imbalance pharmacologically induced by Rotenone triggers behavioral, neural, and inflammatory alterations in the Eisenia fetida earthworm

Mastella

Roggia

Turra

et al. 2021

Preprint

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Background: The inflammatory theory of depression has been tested from epidemiological and experimental investigations. Some studies have suggested that mitochondrial dysfunction superoxide imbalance could increase the susceptibility to chronic stressful events, contributing to the establishment of chronic inflammation and the development of mood disorders. If this premise is true, mitochondrial superoxide imbalance induced by some molecules, such as Rotenone could be evolutionary conservated causing behavioral, immune, and neurological alterations in animals with the primitive central nervous system. Objective: To test this hypothesis, we analyzed some behavioral, immune, and histological markers in Eisenia fetida earthworms chronically exposed to Rotenone, that causes mitochondrial impairment for 14 days. Methods: earthworms were put in an artificial soil containing 30 nM of Rotenone distributed into a plastic cup that allowed the earthworms to leave and return freely into the ground. Since these organisms prefer to be buried in the ground, the model predicted that the earthworm would necessarily have to return to the Rotenone-contaminated medium creating a stressful condition. The effect on survival behavior, in the immune and histological body wall and ventral nervous ganglia (VNG) structures were evaluated, as well gene expression related to inflammation, mitochondrial and neuromuscular changes. Results: Rotenone-induced loss of earthworm escape behavior triggered by boric acid presence; it caused immune alterations indicatives of chronic inflammatory states. Some histological changes in the body wall and VNG indicated a possible earthworm reaction aimed at protection against Rotenone. Overexpression of the nicotinic acetylcholine receptor gene (nAChRs α5) in neural tissues could also help earthworms to reduce the degenerative impact of Rotenone on dopaminergic neurons. Conclusion: The data suggest that mitochondrial dysfunction could be an evolutionarily conserved element in inducing inflammatory and behavioral changes related to exposure to chronic stress.

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Section: Discussionmentioning

confidence: 99%

Superoxide-imbalance pharmacologically induced by Rotenone triggers behavioral, neural, and inflammatory alterations in the Eisenia fetida earthworm

Mastella

Roggia

Turra

et al. 2021

Preprint

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“…The biological effects of caffeine are heterogeneous ( Figure 3). On the one hand, it has been suggested that low doses of caffeine could exert beneficial activities on endothelial function [35,36], in inflammatory processes by reducing some pro-inflammatory cytokines [36,37], and in the improvement of cognitive faculties, as well as providing protection against dementia [35,38]. However, clinical trials conducted in humans have suggested that caffeine is not responsible for the certain beneficial properties observed in caffeinated beverages, like the anti-inflammatory effects of coffee [39,40].…”

Section: Caffeinementioning

confidence: 99%

Caffeine, a Risk Factor for Osteoarthritis and Longitudinal Bone Growth Inhibition

Guillán-Fresco

Franco-Trepat

Alonso-Pérez

et al. 2020

JCM

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Osteoarthritis (OA), the most common chronic rheumatic disease, is mainly characterized by a progressive degradation of the hyaline articular cartilage, which is essential for correct joint function, lubrication, and resistance. Articular cartilage disturbances lead to joint failure, pain, and disability. Hyaline cartilage is also present in the growth plate and plays a key role in longitudinal bone growth. Alterations of this cartilage by diverse pathologies have been related to longitudinal bone growth inhibition (LBGI), which leads to growth retardation. Diet can play a crucial role in processes involved in the OA and LBGI’s onset and evolution. Specifically, there is ample evidence pointing to the negative impacts of caffeine consumption on hyaline cartilage. However, its effects on these tissues have not been reviewed. Accordingly, in this review, we summarize all current knowledge in the PubMed database about caffeine catabolic effects on articular and growth plate cartilage. Specifically, we focus on the correlation between OA and LBGI with caffeine prenatal or direct exposure. Overall, there is ample evidence indicating that caffeine intake negatively affects the physiology of both articular and growth plate cartilage, increasing consumers predisposition to suffer OA and LBGI. As a result, caffeine consumption should be avoided for these pathologies.

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“…Populations living in the Amazon rainforest use herbal plants and products obtained from certain fruits to relieve various clinical symptoms such as inflammation, pain, and fever and for wound healing. This is the case for guarana (Paullinia cupana) seed powder and açai (Euterpe oleracea), which have important antioxidant and anti-inflammatory properties [9][10][11][12][13][14][15]. Studies have shown that these fruits have an effect on the reversal of mitochondrial dysfunctional states and antiaging properties [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Amazonian Guarana- and Açai-Conjugated Extracts Improve Scratched Fibroblast Healing and Eisenia fetida Surgical Tail Amputation by Modulating Oxidative Metabolism

Felin

Maia-Ribeiro

Felin

et al. 2022

Oxidative Medicine and Cellular Longevity

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Background. Previous studies have suggested that guarana (Paullinia cupana) and açai (Euterpe oleracea) have antioxidant, anti-inflammatory, and proliferative properties, indicating their potential therapeutic action in wound healing. We produced a conjugated guarana-açai (GA) extract and tested its healing action on earthworms (Eisenia fetida) subjected to tail amputation by surgical incision. Methods. Extract from roasted guarana seeds and fresh açai seed berries was produced. The antioxidant and genoprotective capacity of GA extract was tested. The concentration with the most remarkable healing potential was used in subsequent tests. The last three posterior segments of the clitellate earthworm tail reared under standardized conditions were surgically amputated. Next, topical PBS or GA extract was applied to the surgical wound. The rate of cell migration and tissue regeneration at the local wound site was histologically evaluated after the procedure. Expression of the SOX4 gene that acts in epithelial-to-mesenchymal transition was determined by RT-qPCR. Results. Sixteen bioactive molecules, including some previously described substances, were identified. All tested concentrations exhibited antioxidant and genoprotective effects. The GA extract accelerated the healing processes as observed through macroscopic and histological analyses and increased expression of SOX4. Conclusion. The GA extract has a potential role in the healing of surgically induced wounds.

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Caffeinated beverages contribute to a more efficient inflammatory response: Evidence from human and earthworm immune cells

Cited by 16 publications

References 42 publications

Superoxide-imbalance pharmacologically induced by Rotenone triggers behavioral, neural, and inflammatory alterations in the Eisenia fetida earthworm

Superoxide-imbalance pharmacologically induced by Rotenone triggers behavioral, neural, and inflammatory alterations in the Eisenia fetida earthworm

Caffeine, a Risk Factor for Osteoarthritis and Longitudinal Bone Growth Inhibition

Amazonian Guarana- and Açai-Conjugated Extracts Improve Scratched Fibroblast Healing and Eisenia fetida Surgical Tail Amputation by Modulating Oxidative Metabolism

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