Caffeine activates preferentially α1-isoform of 5′AMP-activated protein kinase in rat skeletal muscle

Egawa, Tatsuro; Hamada, Taku; Ma, Xiao; Karaike, Kouhei; Kameda, Naoko; Masuda, Shinji; Iwanaka, Nobumasa; Hayashi, Tatsuya

doi:10.1111/j.1748-1716.2010.02169.x

Cited by 57 publications

(81 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, caffeine activates cyclic AMP-dependent protein kinase through an insulin-independent mechanism, which increases AMP kinase-1 to promote glucose transport. Caffeine antagonizes the adenosine A1 receptors in skeletal muscles, which improves glucose tolerance in rats [27]. These mechanisms may partially explain the reported hypoglycemic effect of caffeine in the present study.…”

Section: Discussionsupporting

confidence: 72%

A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

Abunasef

Amin

Abdel-Hamid

2014

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:In this study, the histological, immunohistochemical, morphometric, and biochemical changes to pancreatic beta-cells in STZ-induced diabetes were evaluated in rats treated with different doses of caffeine. Fifty adult male Wistar albino rats were divided into five groups: the nondiabetic control group, the diabetic untreated group, and three diabetic groups treated with different doses of caffeine (10, 50, and 100 mg/kg/day). Blood glucose and serum insulin levels were measured. The pancreata were collected and processed into paraffin sections. They were stained using hematoxylin and eosin (H&E) and Masson trichrome stains. The insulin expression in beta-cells was assessed using immunohistochemistry. Morphometrically, the percentage area of anti-insulin antibody reaction, the percentage of beta-cells per total islet cell number, and the average area of the islets were determined. STZ-induced degenerative changes in beta-cells led to decreases in the number of functioning beta-cells and insulin immunoreactivity and to increases in the number of collagen fibers in the islets. In STZ-treated rats, caffeine significantly decreased blood glucose concentration while increasing blood insulin levels at the highest applied dose. It also induced a significant increase in the number of immunoreactive beta-cells. In conclusion, caffeine may have a protective role in the biochemical and microscopic changes in pancreatic beta-cells in diabetes induced in rats through STZ administration.

show abstract

Section: Discussionsupporting

confidence: 72%

A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

Abunasef

Amin

Abdel-Hamid

2014

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…from the SR (Palade 1987) or affect Ca 2? activation of varied skeletal muscle fibers (Posterino and Dunn 2008), activating the 5 0 AMP-activated protein kinase (AMPK) (Egawa et al 2011;Jensen et al 2007), and reducing plasma K ? levels (Lindinger et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Caffeine’s effect on intermittent sprint cycling performance with different rest intervals

et al. 2011

View full text Add to dashboard Cite

The purpose of this study was to investigate the effects of caffeine ingestion on the performance of an intermittent sprint cycling test (ISCT) with different rest intervals. Fourteen males with team sport experience consumed 6 mg kg(-1) of caffeine or a placebo 60 min prior to completing two sets of an ISCT with 4-min rest intervals. Each set consisted of 12 × 4-s sprints with 20- or 90-s active recovery intervals at 60-70 rpm. Blood lactate was collected at baseline and immediately following the completion of six sprints in each set. At 20-s recovery intervals, peak power and total work were not significantly different between conditions during the ISCT (P > 0.05); but caffeine reduced 6.31% effort for mean power in Sprint 10 of the later stage, as well as an increased fatigue index and elevated blood lactate levels during the ISCT (P < 0.05). At 90-s recovery intervals, peak power, mean power, and total work under caffeine conditions were significantly higher than under placebo conditions during the ISCT (P < 0.05), but no differences were apparent in fatigue index and blood lactate levels (P > 0.05). In conclusion, caffeine ingestion may be ergolytic, affecting performance and fatigue development in the later stage during a prolonged and intermittent sprint test with a short recovery interval. However, caffeine produces an ergogenic effect in the initial stage of an intermittent sprint performance with a longer recovery interval.

show abstract

“…Many epidemiological studies have indicated that long-term coffee consumption is associated with a reduced risk of developing T2DM [19,20]. Our recent studies [21,22] reported that caffeine, the major constituent of coffee, acutely increases AMPKα Thr 172 phosphorylation, an essential step for kinase activation [23] and insulin-independent glucose transport in rat skeletal muscles. Although our results suggest that caffeine is an active compound responsible for the antidiabetic effect of coffee, coffee is a complex mixture of hundreds of chemicals that occur naturally or are formed during the roasting process.…”

Section: Introductionmentioning

confidence: 97%

Coffee polyphenol caffeic acid but not chlorogenic acid increases 5′AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscle

Tsuda

Egawa

et al. 2012

The Journal of Nutritional Biochemistry

View full text Add to dashboard Cite

Caffeine activates preferentially α1-isoform of 5′AMP-activated protein kinase in rat skeletal muscle

Abstract: Our results suggest that of the two α isoforms of AMPK, AMPKα1 is predominantly activated by caffeine via an energy-independent mechanism and that the activation of AMPKα1 increases glucose transport and ACC phosphorylation in skeletal muscle.

Cited by 57 publications

References 61 publications

A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

A histological and immunohistochemical study of beta cells in streptozotocin diabetic rats treated with caffeine

Caffeine’s effect on intermittent sprint cycling performance with different rest intervals

Coffee polyphenol caffeic acid but not chlorogenic acid increases 5′AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscle

Contact Info

Product

Resources

About