Caffeoyl-CoA 3-O-methyltransferase gene family in jute: Genome-wide identification, evolutionary progression and transcript profiling under different quandaries

Abstract

Jute (Corchorus sp.), is a versatile, naturally occurring, biodegradable material that holds the promising possibility of diminishing the extensive use of plastic bags. One of the major components of the cell wall, lignin plays both positive and negative roles in fiber fineness and quality. Although it gives mechanical strength to plants, an excess amount of it is responsible for the diminution of fiber quality. Among various gene families involved in the lignin biosynthesis, Caffeoyl-CoA 3-O-methyltransferase (CCoAOMT) is the most significant and has remained mostly unexplored. In this study, an extensive in-silico characterization of the CCoAOMT gene family was carried out in two jute species (C. capsularis L. and C. olitoroius L.) by analyzing their structural, functional, molecular and evolutionary characteristics. A total of 6 CCoAOMT gene members were identified in each of the two species using published reference genomes. These two jute species showed high syntenic conservation and the identified CCoAOMT genes formed four clusters in the phylogenetic tree. Histochemical assay of lignin in both jute species could shed light on the deposition pattern in stems and how it changes in response to abiotic stresses. Furthermore, expression profiling using qPCR showed considerable alteration of CCoAOMT transcripts under various abiotic stresses and hormonal treatment. This study will lay a base for further analysis and exploration of target candidates for overexpression of gene silencing using modern biotechnological techniques to enhance the quality of this economically important fiber crop.

Cloning and bioinformatics analysis of key gene ShOMT3 of podophyllotoxin biosynthesis pathway in Sinopodophyllum hexandrum

“…OMTs serve as a crucial regulatory enzyme in the phenylpropanoid metabolic pathway, and several OMT enzyme have been identi ed in the podophyllotoxin biosynthetic pathway, including ShCOMT, ShCCoMT, ShOMT1, and ShOMT3 46-48 . ShCOMT is responsible for regulating the conversion of caffeic acid to ferulic acid, while ShCCoMT is responsible for converting caffeoyl-CoA to feruloyl-CoA 49,50 . These two OMT enzyme play an important regulatory role in the synthesis of podophyllotoxin precursor compound pinobanksin, and have been isolated in multiple plants 51,52 .…”

Proteo-metabolomic Dissection of Extracellular Matrix Reveals Alterations in Cell Wall Integrity and Calcium Signaling Governs Wall-Associated Susceptibility during Stem Rot Disease in Jute

“…2 Moreover, the ECM plays an important role in jute fiber quality. 14 Therefore, unraveling the repertoire of extracellular matrix proteins for managing rot fungus would offer insight into key processes that regulate disease or immune response. Various studies to date reported the ECM proteome of plants in response to hemibiotrophic fungal pathogens, particularly Fusarium and Verticillium, and highlighted the role of ECM proteins in disease and defense pathways.…”

“…Besides structural functions, the ECM plays key roles, such as building turgor pressure, governing intercellular communication, and providing a defense response against pathogens . Moreover, the ECM plays an important role in jute fiber quality . Therefore, unraveling the repertoire of extracellular matrix proteins for managing rot fungus would offer insight into key processes that regulate disease or immune response.…”