Caffeoylquinic Acid Derivatives Protect SH-SY5Y Neuroblastoma Cells from Hydrogen Peroxide-Induced Injury Through Modulating Oxidative Status

Abstract

Oxidative stress has been confirmed as a contribution to the pathogenesis and pathophysiology of many neurological disorders such as Alzheimer's disease and Parkinson's disease. Caffeoylquinic acids (CQAs) are considered to have anti-oxidative stress ability in a previous study, but the structure-activity relationships (SARs) of CQAs in neuroprotective effects are still unclear. In the present study, we primarily expound the SARs of CQAs in counteracting HO-induced injury in SH-SY5Y cells. We found that CQAs (1-10) represented the protection of SH-SY5Y cells against HO-induced injury in varying degrees and malonyl groups could obviously increase the anti-oxidative stress ability of CQAs. Intensive studies of 4,5-O-dicaffeoyl-1-O-(malic acid methyl ester)-quinic acid (MDCQA) indicated that the mechanisms could potentially involve activation of endogenous antioxidant enzymes and the regulation of the phosphorylation of MAPKs and AKT. In conclusion, MDCQA could serve as a neuroprotective agent with a potential to attenuate oxidative stress.

Chemical composition, antioxidant potential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis

“… 86 Jiang et al. 87 on amelioration of H 2 O 2 induced neurotoxicity by herbal extracts and phenol/flavonoids.…”

Metal Chelation Therapy and Parkinson’s Disease: A Critical Review on the Thermodynamics of Complex Formation between Relevant Metal Ions and Promising or Established Drugs

“…Creatine [179,180] Cudraflavone B [181] Curcumin [89,117,[182][183][184] Cyanidin [185,186] D-512 [187] D-607 (bipyridyl-D2R/D3R agonist hybrid) [12,188,189] DA-2 (8D) [12,89] DA-3 [12] DA-4 [12] Dabigatran etexilate [190] Dabrafenib [191] (S)-3,4-DCPG [115] Deferasirox [24] Deferricoprogen [192] Delphinidin [160,185,193,194] Demethoxycurcumin [195] Dendropanax morbifera active compound [196] Desferrioxamine (Desferoxamine, Desferal, DFO) [112] (S)-N-(3-(3,6-Dibromo-9H -carbazol-9-yl)-2-fluoropropyl)-6-methoxypyridin-2-amine [197] 4,5-O-Dicaffeoyl-1-O-(malic acid methyl ester)-quinic acid derivatives (R1, R2, R3, R4, or R5 = caffeoyl) [198] Dihydromyricetin [199]…”

Thromboxane A2 receptor antagonist SQ29548 attenuates SH‑SY5Y neuroblastoma cell impairments induced by oxidative stress

“…SH-SY5Y cells were selected in the present study as the in vitro human nerve cell model, while SQ29548 was used as a TXA2R antagonist and H 2 O 2 was used to simulate oxidative stress in vitro. SH-SY5Y, a neuron cell line, is widely used in studies investigating neurological disorders (27,28) and oxidative stress in nerve cells (17,28). SQ29548 has been proposed to be a specific, reliable and safe TXA2R antagonist (29), and is broadly utilized in studies on TXA2R (7,25,30,31).…”

“…1c). considering other relevant findings (17,23), the concentration of 0.1 mM H 2 O 2 was selected for subsequent experiments. (Fig.…”

“…Numerous studies have proven that the elevation of cellular antioxidative stress capacity was beneficial for the limitation of ROS generation, acceleration of ROS elimination, alleviation of ROS damage and inhibition of MAPK signaling pathways to reduce cell death (13,16). compared with other organs (17), brain tissue is sensitive to oxidative stress damage due to its characteristics of high energy consumption and weak antioxidative stress capacity. Therefore, the identification of a novel effective neuroprotective agent is essential in the prevention and treatment of oxidative stress-associated neurological disorders.…”