Caftaric Acid Ameliorates Oxidative Stress, Inflammation, and Bladder Overactivity in Rats Having Interstitial Cystitis: An In Silico Study

Saima, .; Anjum, İrfan; Najm, Saima; Barkat, Kashif; Nafidi, Hiba-Allah; Jardan, Yousef A. Bin; Bourhia, Mohammed

doi:10.1021/acsomega.3c01450

Cited by 6 publications

(1 citation statement)

References 47 publications

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“…IC 50 values were calculated by graphical method. All the data was analyzed using Graph pad prism version 8.0.2 [23,24].…”

Section: Discussionmentioning

confidence: 99%

Canagliflozin produces a vasorelaxation effect on isolated rat thoracic aorta via NO/cGMP pathways

Tariq,

Anjum,

Mushtaq

et al. 2023

Preprint

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Canagliflozin is a sodium-glucose cotransporter 2 inhibitor (SGLT2i) an oral hypoglycemic agent. The present study was performed to determine the vasorelaxant response of canagliflozin along with the underlying mechanism. In diabetes-associated complications, those involving blood vessels remain a high cause of morbidity. The diabetes-related cardiovascular complications should be treated with newer drugs that could cure both complications concurrently. The study was conducted on the aortic rings of Wistar rats of either sex. Canagliflozin (10-8-10-4 M) induces relaxation in phenylephrine (PE 100 µM) and 80 mM KCl pre-contracted rings significantly in a cumulative manner acquiring endothelium. The canagliflozin mechanism of vasorelaxant was established by incubating endothelium intact aortic rings for 30 minutes before PE with; nitro-L-arginine methyl ester (L-NAME 100 µM), methylene blue (10 µM), barium chloride (BaCl2: 10 µM), glibenclamide (1 µM) and indomethacin (10 µM). To estimate the role of calcium (Ca+ 2), cumulative Ca2+ (0.01- 10.0 mM) was added in a bath containing Ca+ 2 free Krebs-Henseleit solution. It was repeated by pre-incubating strips with nifedipine (1 µM) and canagliflozin (1 µM, 10 µM and 100 µM) respectively. The relaxant response of canagliflozin involves the release of nitric oxide, cyclic guanosine monophosphate, prostacyclin, and membrane hyperpolarization from endothelium as pretreatment with L-NAME (IC50: 2.166 × 10− 7 ± 7 M ), methylene blue (IC50 : 1.738 × 10− 7 ± 7 M), indomethacin (IC50: 2.269 × 10− 7± 7 M), BaCl2 (IC50 : 3.320 × 10− 6 ± 7 M) and glibenclamide (IC50: 3.960 × 10− 7 ± 7 M) diminished the response. Additionally, canagliflozin relaxing effects include both decreased Ca+ 2 influx and release from sarcoplasmic reserves. Thus, canagliflozin is a possible adjuvant in diabetes with concurrent hypertension.

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“…IC 50 values were calculated by graphical method. All the data was analyzed using Graph pad prism version 8.0.2 [23,24].…”

Section: Discussionmentioning

confidence: 99%

Canagliflozin produces a vasorelaxation effect on isolated rat thoracic aorta via NO/cGMP pathways

Tariq,

Anjum,

Mushtaq

et al. 2023

Preprint

Self Cite

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Cuminum cyminum Ameliorates Urotoxic Effects of Cyclophosphamide by Modulating Antioxidant, Inflammatory Cytokines, and Urinary Bladder Overactivity: In vivo and in Silico Investigations

Anjum,

Ali,

Bourhia

et al. 2023

Chemistry & Biodiversity

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This study aimed to investigate the effect of aqueous ethanol extract of Cuminum cyminum (AEECC) on oxidative stress, inflammation and overactivity of the urinary bladder induced by cyclophosphamide (CYP). The enhanced nociception behavior, bladder weight, vascular permeability, edema, hemorrhage, nitric oxide, IL‐6 and TNF‐α levels by CYP administration were significantly reduced by AEECC (250 and 500 mg/kg). A significant increase in serum antioxidant systems such as CAT and GPx was also observed in AEECC‐treated rats. The AEECC (3 mg/ml) significantly reduced urinary bladder tone in the strips pre‐contracted with carbachol in both control and CYP‐treated rats. This relaxation was demolished by atropine, nifedipine, glibenclamide, and indomethacin but not with propranolol. The plant extract showed the presence of antioxidant and anti‐inflammatory phytochemicals. These results suggest that Cuminum cyminum offers uroprotective activity and can ameliorate CYP‐induced bladder toxicity by modulating antioxidant parameters, pro‐inflammatory cytokine levels and bladder smooth muscle overactivity. The in silico binding interactions of anti‐oxidant 2I3Y and anti‐inflammatory protein 1TNF with various ligands from Cuminum cyminum seeds reveal potential bioactive compounds with promising anti‐oxidant and anti‐inflammatory properties, providing valuable insights for drug development and nutraceutical research

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Efficient synthesis and molecular docking analysis of quinazoline and azole hybrid derivatives as promising agents for anti-cancer and anti-tuberculosis activities

Kumar,

Soni

et al. 2024

Journal of Molecular Structure

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Caftaric Acid Ameliorates Oxidative Stress, Inflammation, and Bladder Overactivity in Rats Having Interstitial Cystitis: An In Silico Study

Cited by 6 publications

References 47 publications

Canagliflozin produces a vasorelaxation effect on isolated rat thoracic aorta via NO/cGMP pathways

Canagliflozin produces a vasorelaxation effect on isolated rat thoracic aorta via NO/cGMP pathways

Cuminum cyminum Ameliorates Urotoxic Effects of Cyclophosphamide by Modulating Antioxidant, Inflammatory Cytokines, and Urinary Bladder Overactivity: In vivo and in Silico Investigations

Efficient synthesis and molecular docking analysis of quinazoline and azole hybrid derivatives as promising agents for anti-cancer and anti-tuberculosis activities

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