Cage‐cultured Largemouth Bronze Gudgeon, Coreius guichenoti: Biochemical Profile of Plasma and Physiological Response to Acute Handling Stress

Abstract

We examined the blood biochemical profile and the physiological response to acute handling stress of a historically important economic but currently a rare endangered fish, Largemouth bronze gudgeon, Coreius guichenoti , in the upper Yangtze River. The results showed that the glucose (GLU) and triglyceride (TG) levels in the cage-cultured C. guichenoti were significantly higher than those of the wild type, and the basal cortisol (COR) level was higher than that of most other fish species. In the stress experiment, the fish were subjected to a low-and high-intensity handling stress, respectively, and sampling was performed before the stress and at 0, 1, 4, 12, 24, 72, 120, and 168 h after the stress. The levels of COR, GLU, lactate (LA), and alanine aminotransferase (ALT) of stressed fish exhibited an increase first and then a recovery to the pre-stress levels with a typical acute stress response in the first 24 h. Fish in both stressed groups were spontaneously infected with Ichthyophthirius multifiliis from the 24th to the 168th h, and exhibited a chronic stress response. These data would facilitate the studies of blood physiology of C. guichenoti and related fish in health evaluation, species propagation and conservation, as well as the stress response in aquaculture.

Cooling combined with hyperoxic CO2 anesthesia is effective in improving the air exposure duration of tilapia

“…In the present experiment, increases in AST and ALT were induced by AE rather than temperature drop because there were no significant changes in the CO&AN&CT treatment, while they significantly increased in the CO&AN&AE treatment. Similar results were also noted in the common carp 23 and Largemouth Bronze Gudgeon 22 after air exposure.…”

“…The present data illustrated that the AE treatment elicited a much more severe stress response compared with the CT treatment, as most parameters dramatically changed after the AE treatment (Table 1 ). Similar results were also found in Largemouth Bronze Gudgeon ( Coreius guichenoti ) 22 and cobia 20 challenged to air exposure for 1 min. Interestingly, lactate returned to its basal value in the CT treatment, whereas it subsequently increased in the AE treatment, because lactate caused by CO generally lasted short term and recovered over a short period 12 .…”

“…Physiological responses during air exposure resulted in elevated cortisol, which was a predictor of mortality 1 . Although the cortisol responses were different among species and experiment protocols, the value of cortisol in tilapia (186 nmol/L, Table 1 ) was less than those in other species after air exposure, such as 883 nmol/L in Largemouth Bronze Gudgeon 22 and 634.8 nmol/L in cobia 20 . The limited cortisol response might explain why anesthetized tilapia had prolonged air exposure duration.…”

Effects of Temperature Reduction and MS‐222 on Water Quality and Blood Biochemistry in Simulated Transport Experiment of Largemouth Bronze Gudgeon, Coreius guichenoti

“…At the end of transport, the increase in AST concentrations was significant compared with the control group, though it was not significantly different between the four treatments, suggesting that ALT is more sensitive than AST as a stress parameter for evaluating the stress response to the long-term transportation of C. guichenoti. This hypothesis has been tested in an acute air exposure in C. guichenoti (Zhao et al 2013).…”

Physiological responses to swimming fatigue in juvenile largemouth bronze gudgeon Coreius guichenoti

“…Monitoring blood biochemistry is used to evaluate metabolic and nutritional status, and the intensity of responses to stress (Barcellos et al, ; Faleiro, ). Changes in plasma glucose (Glu) and triglyceride (TG) levels positively correlate with stressor magnitude and response intensity (Zhao et al, ).…”