Caged midge larvae (<i>Chironomus riparius</i>) for the assessment of metal bioaccumulation from sediments in situ

Bervoets, Lieven; Meregalli, Giovanna; Cooman, Ward De; Goddeeris, Boudewijn; Blust, Ronny

doi:10.1897/03-1

Cited by 36 publications

(19 citation statements)

References 40 publications

(68 reference statements)

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“…Handling and density of test organisms —General handling, transfer, and transport of test organisms and the number of test organisms per cage or experimental unit (density) are important considerations (Chappie and Burton 1997, 2000). Clearly, the density of test organisms within a chamber should not be so great that it affects the endpoints measured (Ringwood and Keppler 2002; Den Besten et al 2003; Bervoets et al 2004). Food and habitat (space) limitation, as well as intraspecies predation (cannibalism), can create further problems.…”

Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

“…Food and habitat (space) limitation, as well as intraspecies predation (cannibalism), can create further problems. Intuitively, organisms should be handled as little as possible, and care should be taken during introduction to test chambers (Sibley et al 1999; Bervoets et al 2004). For example, a common method for introducing amphipods and chironomids to test containers is via careful syringe injection though container ports (Kater et al 2001; Castro et al 2003).…”

Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

“…In bioassays using benthic organisms such as chironomids, sediments may have to be sieved to remove indigenous species, however, this also likely will alter contaminant concentrations and bioavailability (Crane et al 2000; Simpson and Batley 2003; Bervoets et al 2004). Freezing has been used successfully as an alternative means to remove (kill) indigenous macroinvertebrates in sediment prior to use of that sediment in colonization trays (Kline and Stekoll 2001; Trannum et al 2004; Liber et al 2005; Pettigrove and Hoffman 2005).…”

Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

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In situ‐based effects measures: Considerations for improving methods and approaches

Liber

Goodfellow²,

Besten³

et al. 2007

Integr Envir Assess & Manag

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In situ‐based effects measures have gained increased acceptance as a means to improve the link between cause and effect in aquatic ecotoxicological studies. These approaches have primarily been employed where more conventional laboratory tests with field collected samples and routine in‐field community surveys have failed to provide reasonable answers with respect to causes of toxicity, primary routes of contaminant exposure, and what constitutes ecotoxicologically relevant contaminant levels, at least at a site‐specific level. One of the main advantages provided by in situ tests compared to more conventional field‐based monitoring approaches is that they provide better control over stressor exposure to a defined population of test animals under natural or near‐natural field conditions. In situ techniques can also be used to avoid artifacts related to sampling, transport and storage of contaminated water and sediment intended for laboratory‐based toxicity assessment. In short, they can reduce the need for laboratory to field extrapolation and, when conducted properly, in situ tests can provide improved diagnostic ability and high ecological relevance. This paper provides suggestions and considerations for designing in situ studies, choosing test species and test endpoints, avoiding or minimizing test artifacts, best addressing some of the limitations of in situ test techniques, and generally improving the overall quality of the in situ approach chosen.

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Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

Section: Important Issues Challenges and Considerations For Conductimentioning

confidence: 99%

See 1 more Smart Citation

In situ‐based effects measures: Considerations for improving methods and approaches

Liber

Goodfellow²,

Besten³

et al. 2007

Integr Envir Assess & Manag

View full text Add to dashboard Cite

show abstract

“…This confirms that D is an important contamination source of SN. Copper and Ni were abundant in K, but concentrations were seven to 40 times lower than in 2004 [24] and three to nine times lower than in 2010 [25]. The water quality of K therefore appears to have improved, although the concentrations of Cu and Ni were still eight (Cu) and 1.5 (Ni) times above the surface water quality standards.…”

Section: Discussionmentioning

confidence: 99%

Evaluating Complex Mixtures in the Zebrafish Embryo by Reconstituting Field Water Samples: A Metal Pollution Case Study

Michiels

Vergauwen

Hagenaars

et al. 2017

IJMS

Self Cite

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Accurately assessing the toxicity of complex, environmentally relevant mixtures remains an important challenge in ecotoxicology. The goal was to identify biological effects after exposure to environmental water samples and to determine whether the observed effects could be explained by the waterborne metal mixture found in the samples. Zebrafish embryos were exposed to water samples of five different sites originating from two Flemish (Mol and Olen, Belgium) metal contaminated streams: “Scheppelijke Nete” (SN) and “Kneutersloop” (K), and a ditch (D), which is the contamination source of SN. Trace metal concentrations, and Na, K, Mg and Ca concentrations were measured using ICP-MS and were used to reconstitute site-specific water samples. We assessed whether the effects that were observed after exposure to environmental samples could be explained by metal mixture toxicity under standardized laboratory conditions. Exposure to “D” or “reconstituted D” water caused 100% mortality. SN and reconstituted SN water caused similar effects on hatching, swim bladder inflation, growth and swimming activity. A canonical discriminant analysis confirmed a high similarity between both exposure scenarios, indicating that the observed toxicity was indeed primarily caused by metals. The applied workflow could be a valuable approach to evaluate mixture toxicity that limits time and costs while maintaining biological relevance.

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“…(e.g. Bervoets et al 2004) and a similar procedure could be tailored for chronic toxicity tests with T. tubifex or L. variegatus, or any other oligochaete species appropriate for the in-situ conditions.…”

Section: In Situ Bioassaysmentioning

confidence: 99%

Toxicology and Laboratory Studies

Rodríguez

Reynoldson

2011

The Pollution Biology of Aquatic Oligochaetes

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This chapter provides a chronological synthesis of the available literature and information on laboratory based studies of aquatic oligochaetes and has been organized into water-only vs. sediment toxicity tests, and acute vs. chronic toxicity tests. Toxicological studies have employed relatively few species. Absence of sediment in tests with aquatic oligochaetes is a source of stress and reduces realism in exposure conditions and most current toxicity work is performed in the presence of sediment. Thus, unless there is a requirement for water-only tests, for interspecies comparison of toxicity of chemical compounds, or for routine control of species sensitivity in laboratory cultures, these tests are considered inappropriate for oligochaete worms in ecological risk assessment. Field populations of several species have been demonstrated to vary genetically, and may have different tolerances to both environmental and anthropogenic stress. This suggests that test organisms should be obtained from cultures of a defined genetic strain, or alternatively the sensitivity of the populations should be intercalibrated with reference toxicants. Oligochaete worms are not uniformly more tolerant to contaminants than other test organisms. Comparative data for worms and other benthic invertebrates show that responses are species-specific and also contaminant-specific. Among the sublethal measurements, sexual reproduction seems to be the most easily standardised and informative endpoint, and has high ecological relevance, since reproductive impairment has the same long-term effect as mortality on the population. Sublethal, shortterm responses such as behavioural responses (avoidance behaviour, sediment reworking and sediment borrowing) need further standardization. In the future, in situ bioassays are promising tools in environmental risk assessment but will require the use of species characteristic of particular habitats or that are suitable for reproduction and toxicity assessment under different environmental conditions.

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Caged midge larvae (Chironomus riparius) for the assessment of metal bioaccumulation from sediments in situ

Cited by 36 publications

References 40 publications

In situ‐based effects measures: Considerations for improving methods and approaches

In situ‐based effects measures: Considerations for improving methods and approaches

Evaluating Complex Mixtures in the Zebrafish Embryo by Reconstituting Field Water Samples: A Metal Pollution Case Study

Toxicology and Laboratory Studies

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