Caged protein nanoparticles for drug delivery

Molino, N; Wang, Szu‐Wen

doi:10.1016/j.copbio.2013.12.007

Cited by 164 publications

(131 citation statements)

References 67 publications

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“…121 Hydrophobic drug molecules, peptides, and gene drugs are covalently conjugated to the hollow cavity of the protein scaffold or noncovalently loaded into the internal space. [122][123][124][125] Generally, restricted by the number of attachment sites, most of the protein NP-based nanomedicines prepared by conventional chemical conjugation present the same drawback of lower drug-loading content (,10%).…”

Section: Protein Nps As Carriersmentioning

confidence: 99%

“…When these nanomedicines are administered for the second time, elevated immune responses can occur and induce rapid clearance or neutralization or create potentially severe inflammatory responses. 121 One way to bypass this problem is surface functionalization by introducing PEG and other coatings to reduce immunorecognition. 71,72 The other is harnessing this natural effect and using it in immunobased treatments.…”

Section: Protein Nps As Carriersmentioning

confidence: 99%

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High drug-loading nanomedicines: progress, current status, and prospects

Shen

Liu

et al. 2017

IJN

438

268

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Drug molecules transformed into nanoparticles or endowed with nanostructures with or without the aid of carrier materials are referred to as “nanomedicines” and can overcome some inherent drawbacks of free drugs, such as poor water solubility, high drug dosage, and short drug half-life in vivo. However, most of the existing nanomedicines possess the drawback of low drug-loading (generally less than 10%) associated with more carrier materials. For intravenous administration, the extensive use of carrier materials might cause systemic toxicity and impose an extra burden of degradation, metabolism, and excretion of the materials for patients. Therefore, on the premise of guaranteeing therapeutic effect and function, reducing or avoiding the use of carrier materials is a promising alternative approach to solve these problems. Recently, high drug-loading nanomedicines, which have a drug-loading content higher than 10%, are attracting increasing interest. According to the fabrication strategies of nanomedicines, high drug-loading nanomedicines are divided into four main classes: nanomedicines with inert porous material as carrier, nanomedicines with drug as part of carrier, carrier-free nanomedicines, and nanomedicines following niche and complex strategies. To date, most of the existing high drug-loading nanomedicines belong to the first class, and few research studies have focused on other classes. In this review, we investigate the research status of high drug-loading nanomedicines and discuss the features of their fabrication strategies and optimum proposal in detail. We also point out deficiencies and developing direction of high drug-loading nanomedicines. We envision that high drug-loading nanomedicines will occupy an important position in the field of drug-delivery systems, and hope that novel perspectives will be proposed for the development of high drug-loading nanomedicines.

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Section: Protein Nps As Carriersmentioning

confidence: 99%

Section: Protein Nps As Carriersmentioning

confidence: 99%

High drug-loading nanomedicines: progress, current status, and prospects

Shen

Liu

et al. 2017

IJN

438

268

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“…Furthermore, genetic manipulation allows the creation of modular proteins with disparate functionalities that can be brought together into nanosystems assembled using these synthetic polypeptides. NPs made by the selfassembly of viral capsid proteins [11,12], or of the major vault protein [13] constitute examples of this.…”

Section: Biomolecules As Core Nanoparticle Scaffoldsmentioning

confidence: 99%

‘Smartening’ anticancer therapeutic nanosystems using biomolecules

Núñez-Lozano

Pimentel

Cueva-Méndez

2015

Current Opinion in Biotechnology

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“…14 Ferritin is a major iron storage protein in humans and most living organisms. The ferritin nanocage is composed of 24 subunits, which self-assemble to form a cage-like nanostructure.…”

Section: Introductionmentioning

confidence: 99%

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

Yao¹,

Su²,

Zeng³

et al. 2016

IJN

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Carbon dots (CDs) are one of the most highlighted carbon-based materials for biological applications, such as optical imaging nanoprobes, which are used for labeling cells in cancer treatment mainly due to their biocompatibility and unique optical properties. In this study, gadolinium (Gd)-complex-containing CDs were obtained through a one-step microwave method to develop multimodal nanoprobes integrating the advantages of optical and magnetic imaging. The obtained Gd-CDs exhibited highly fluorescent properties with excellent water solubility and biological compatibility. Natural apoferritin (AFn) nanocages, an excellent drug delivery carrier, are hollow in structure, with their pH-dependent, unfolding–refolding process at pH 2.0 and 7.4. The chemotherapeutic drug doxorubicin (DOX) can be highly effective and encapsulated into AFn cavity. A widely used tumor-targeting molecule, folic acid (FA), functionalized the surface of AFn to obtain an active tumor targeting effect on MCF-7 cells and malignant tumors in mice models. In this study, an AFn nanocarrier encapsulating high concentration of DOX labeled with magnetic and fluorescent Gd-CDs probe was developed. Gd-CDs exhibited a unique green photoluminescence and almost no toxicity compared with free GdCl 3 . Furthermore, Gd-doped CDs significantly increased the circulation time and decreased the toxicity of Gd 3+ in in vitro and in vivo magnetic resonance imaging, which demonstrated that the AFn nanocages labeled with Gd-CD compounds could serve as an excellent T 1 contrast agent for magnetic resonance imaging. The self-assembling multifunctional Gd-CDs/AFn (DOX)/FA nanoparticles have a great potential for cancer theranostic applications.

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Caged protein nanoparticles for drug delivery

Cited by 164 publications

References 67 publications

High drug-loading nanomedicines: progress, current status, and prospects

High drug-loading nanomedicines: progress, current status, and prospects

‘Smartening’ anticancer therapeutic nanosystems using biomolecules

Construction of magnetic-carbon-quantum-dots-probe-labeled apoferritin nanocages for bioimaging and targeted therapy

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