CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum

Singh, Samar; Pal, Lalita; Rajput, Ruchika; Chhatwal, Himani; Singh, Nidhi; Chattopadhyay, Debasis; Pandey, Ashutosh

doi:10.1007/s00425-024-04470-7

Planta

2024

DOI: 10.1007/s00425-024-04470-7

|View full text |Cite

CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum

Samar Singh,

Lalita Pal,

Ruchika Rajput

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

TaCCS1‐B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis

Tyagi,

Shumayla,

Singh

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Abiotic stress, including osmotic and salinity stress, significantly affects plant growth and productivity. Copper chaperone for superoxide dismutase (CCS) is essential for copper homeostasis and oxidative stress management. In this study, we investigated the role of the TaCCS1‐B gene of bread wheat in enhancing stress tolerance in yeast and transgenic Arabidopsis. Expression of TaCCS1‐B increased abiotic stress tolerance in recombinant yeast cells. Phenotypic analysis of Arabidopsis TaCCS1‐B expressing lines demonstrated that they exhibited significantly higher germination rates, increased root length and better growth under osmotic and salinity stress than wild type. Additionally, the transgenic lines exhibited higher copper accumulation and enhanced photosynthetic pigments and proline level, coupled with reduced hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) contents. They also showed higher enzymatic antioxidants' activities, indicating reduced oxidative stress in transgenic lines, resulting in reduced flavonoid content. Gene expression analysis indicated modulated expression of stress‐responsive genes in the transgenic lines under stress conditions. These findings suggested the role of TaCCS1‐B in enhancing stress tolerance by improving copper homeostasis and regulating key stress‐responsive genes. This study highlights the potential of TaCCS1‐B for the development of better stress resilience crops, which is critical for sustaining agricultural productivity for food security under adverse environmental conditions.

show abstract

TaCCS1‐B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis

Tyagi,

Shumayla,

Singh

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

The bHLH Transcription Factor PubHLH66 Improves Salt Tolerance in Daqing Poplar (Populus ussuriensis)

Li,

Wang,

Pan

et al. 2024

Forests

View full text Add to dashboard Cite

Elevated salinity negatively impacts plant growth and yield, presenting substantial challenges to agricultural and forestry productivity. The bHLH transcription factor family is vital for plants to cope with various abiotic stresses. However, it remains uncertain whether bHLH transcription factors can regulate salt stress in Populus ussuriensis. In the following study, a salt-induced bHLH transcription factor PubHLH66 was identified from P. ussuriensis. PubHLH66 has a typical and conserved bHLH domain. Subcellular localization and yeast two-hybrid (Y2H) assays confirmed that it is a nucleus-localized transactivator and the activation region is located at the N-terminus. PubHLH66-OE and PubHLH66-SRDX transgenic P. ussuriensis were obtained through Agrobacterium-mediated leaf disc transformation. Morphological and physiological results demonstrated that PubHLH66-OE enhanced salinity tolerance, as indicated by reduced electrolyte leakage (EL), malondialdehyde (MDA), and H2O2 levels, along with increased proline contents and activities of peroxidase (POD) and superoxide dismutase (SOD). In contrast, PuHLH66-SRDX poplar showed decreased salt tolerance. Quantitative real-time PCR (RT-qPCR) confirmed that PubHLH66 enhanced salt tolerance by regulating the expression of genes such as PuSOD, PuPOD, and PuP5CS, resulting in reduced reactive oxygen species (ROS) accumulation and an improved osmotic potential. Thus, PubHLH66 could be a candidate gene for molecular breeding to enhance salt tolerance in plants. These results laid a foundation for exploring the mechanisms of salt tolerance in P. ussuriensis, facilitating the development of more salt-tolerant trees to combat the increasing issue of soil salinization globally.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum

Cited by 2 publications

References 94 publications

TaCCS1‐B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis

TaCCS1‐B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis

The bHLH Transcription Factor PubHLH66 Improves Salt Tolerance in Daqing Poplar (Populus ussuriensis)

Contact Info

Product

Resources

About