Calbindin D-28k Immunoreactivity and Its Protein Level in Hippocampal Subregions During Normal Aging in Gerbils

Lee, Choong Hyun; Hwang, In Koo; Yoo, Ki‐Yeon; Choi, Jung Hoon; Park, Ok Kyu; Lee, Jae Chul; Jeong, Young‐Gil; Lee, In Se; Won, Moo‐Ho

doi:10.1007/s10571-009-9360-z

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…The hyperpolarization-activated current (I h ) that underlies the sag potential develops in the first three postnatal weeks, and it is comparable to adults by P20 days ( Vasilyev and Barish, 2002 ). The decrease of the sag ratio we observed over age is in line with decreased I h in aged gerbils which reflected a reduction in HCN1 and 2 in the hippocampus ( Lee et al, 2019 ). The smaller sag ratio observed in the aged group both with and without carbachol ( Figs.…”

Section: Discussionsupporting

confidence: 76%

Persistent Firing in Hippocampal CA1 Pyramidal Cells in Young and Aged Rats

Brahimi

Knauer²,

Price³

et al. 2023

eNeuro

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Persistent neuronal firing is often observed in working memory and temporal association tasks both in humans and animals, and is believed to retain necessary information in these tasks. We have reported that hippocampal CA1 pyramidal cells are able to support persistent firing through intrinsic mechanisms in the presence of cholinergic agonists. However, it still remains largely unknown how persistent firing is affected by the development of animals and aging. Using in vitro patch-clamp recordings from CA1 pyramidal cells in rat brain slices, we first show that the cellular excitability of these aged rats was significantly lower than the young rats, responding with fewer spikes to current injection. In addition, we found age-dependent modulations of input resistance, membrane capacitance, and spike width. However, persistent firing in aged (~2 years old) rats was as strong as that in young animals, and the properties of persistent firing were very similar among different age groups. In addition, spike after-hyperpolarization potential (AHP), was not increased by aging and did not correlate with the strength of persistent firing. Lastly, we estimated the depolarization current induced by the cholinergic activation. This current was proportional to the increased membrane capacitance of the aged group and was inversely correlated with the intrinsic excitability of cells. These observations indicate that robust persistent firing can be maintained in aged rats despite reduced excitability, due to the increased amount of cholinergically induced positive current.Significant statement:In an aging society, it is crucial to understand neural mechanisms underlying age-dependent cognitive impairments. In recent years, the importance of intrinsic cellular properties in cognitive functions such as memory has increasingly been recognized. However, research examining age-dependent alteration of intrinsic cellular mechanisms of persistent firing, which is believed to support working memory function, has so far been very scarce. In this study, we demonstrate that the ability to support persistent firing is kept intact in neurons from old rats, despite changes in other properties such as intrinsic excitability. These results identify the ability to support persistent firing as one potential cellular mechanism of rescued cognitive functions under cholinergic enhancement used in Alzheimer's disease and other dementias.

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Section: Discussionsupporting

confidence: 76%

Persistent Firing in Hippocampal CA1 Pyramidal Cells in Young and Aged Rats

Brahimi

Knauer²,

Price³

et al. 2023

eNeuro

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“…Bu et al ( 16 ) reported that the number of CB-immunoreactive neurons in whole layers (full cortical depth columns) of the primary visual cortex exhibited a consistent trend toward a decrease in the aged human brain. In addition, previous studies demonstrated a significant age-associated decrease in CB mRNA and CB immunoreactivity in the striatum ( 13 , 25 ) and hippocampus ( 30 , 31 ) of rodents during aging. These studies and the results of the present study indicate that CB-immunoreactive interneurons in the rodent somatosensory cortex may be affected by age, and their numbers in the aged somatosensory cortex are decreased when compared with those in the adult.…”

Section: Discussionmentioning

confidence: 81%

Immunoreactivities of calbindin-D28k, calretinin and parvalbumin in the somatosensory cortex of rodents during normal aging

Ahn

Hong

Park

et al. 2017

Molecular Medicine Reports

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Calbindin-D28k (CB), calretinin (CR) and parvalbumin (PV), which regulate cytosolic free Ca2+ concentrations in neurons, are chemically expressed in γ-aminobutyric acid (GABA)ergic neurons that regulate the degree of glutamatergic excitation and output of projection neurons. The present study investigated age-associated differences in CB, CR and PV immunoreactivities in the somatosensory cortex in three species (mice, rats and gerbils) of young (1 month), adult (6 months) and aged (24 months) rodents, using immunohistochemistry and western blotting. Abundant CB-immunoreactive neurons were distributed in layers II and III, and age-associated alterations in their number were different according to the species. CR-immunoreactive neurons were not abundant in all layers; however, the number of CR-immunoreactive neurons was the highest in all adult species. Many PV-immunoreactive neurons were identified in all layers, particularly in layers II and III, and they increased in all layers with age in all species. The present study demonstrated that the distribution pattern of CB-, CR- and PV-containing neurons in the somatosensory cortex were apparently altered in number with normal aging, and that CB and CR exhibited a tendency to decrease in aged rodents, whereas PV tended to increase with age. These results indicate that CB, CR and PV are markedly altered in the somatosensory cortex, and this change may be associated with normal aging. These findings may aid the elucidation of the mechanisms of aging and geriatric disease.

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“…CB is present in many double bouquets, bitufted, bipolar, multipolar, and horizontal (Cajal-Retzius) cells [2-7] as well as pyramidal and nonpyramidal neurons in the hippocampus [2,12]. Lee et al reported that younger gerbils (1 month old) show weaker CB expression in the hippocampal CA1 region compared to older gerbils [17]. In the present study, CB-specific immunoreactivity in CA2/CA3 mossy fibers, granule cell fibers, and the DG gradually decreased; however, expression in the DG was increased at STZ4w.…”

Section: Discussionmentioning

confidence: 99%

Time-dependent changes of calbindin D-28K and parvalbumin immunoreactivity in the hippocampus of rats with streptozotocin-induced type 1 diabetes

2013

J Vet Sci

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The hippocampus is affected by various stimuli that include hyperglycemia, depression, and ischemia. Calcium-binding proteins (CaBPs) have protective roles in the response to such stimuli. However, little is known about the expression of CaBPs under diabetic conditions. This study was conducted to examine alterations in the physiological parameters with type 1 diabetes induced with streptozotocin (STZ) as well as time-dependent changes in the expression of two CaBPs changes of were being evaluated. Rats treated with STZ (70 mg/kg) had high blood glucose levels (>21.4 mmol/L) along with increased food intake and water consumption volumes compared to the sham controls. In contrast, body weight of the animals treated with STZ was significantly reduced compared to the sham group. CB-specific immunoreactivity was generally increased in the hippocampal CA1 region and granule cell layer of the dentate gyrus (DG) 2 weeks after STZ treatment, but decreased thereafter in these regions. In contrast, the number of PV-immunoreactive neurons and fibers was unchanged in the hippocampus and DG 2 weeks after STZ treatment. However, this number subsequently decreased over time. These results suggest that CB and PV expression is lowest 3 weeks after STZ administration, and these deficits lead to disturbances in calcium homeostasis.

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Calbindin D-28k Immunoreactivity and Its Protein Level in Hippocampal Subregions During Normal Aging in Gerbils

Cited by 10 publications

References 36 publications

Persistent Firing in Hippocampal CA1 Pyramidal Cells in Young and Aged Rats

Persistent Firing in Hippocampal CA1 Pyramidal Cells in Young and Aged Rats

Immunoreactivities of calbindin-D28k, calretinin and parvalbumin in the somatosensory cortex of rodents during normal aging

Time-dependent changes of calbindin D-28K and parvalbumin immunoreactivity in the hippocampus of rats with streptozotocin-induced type 1 diabetes

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