Calbindin-D9K Gene Expression in Rat Chorioallantoic Placenta Is Not Regulated by 1,25-Dihydroxyvitamin D3

Glazier, Jocelyn D.; Mawer, E. B.; Sibley, Colin P.

doi:10.1203/00006450-199506000-00008

Cited by 45 publications

(29 citation statements)

References 25 publications

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“…However, despite the 1,25(OH) 2 D 3 -responsive element in the calbindin-D 9K gene (Darwish & DeLuca 1992), placental calbindin-D 9K expression is not dependent on this hormone (Glazier et al 1995). It seems likely, therefore, that factors other than vitamin D 3 metabolites are involved in activating the renal and placental calbindin genes in diabetic pregnancy.…”

Section: 25(oh) 2 Dmentioning

confidence: 87%

“…One study has reported that maternal renal concentrations of calbindin-D 9K and calbindin-D 28K proteins at term were comparable between control and genetically diabetic rats (Verhaeghe et al 1988b). The regulation of expression of calbindins in these two calcium-transporting epithelia is distinct: whereas renal calbindin expression is dependent on serum vitamin D 3 metabolite concentrations (Thomasset et al 1982), placental calbindin-D 9K expression is apparently not regulated by this hormone (Glazier et al 1995).…”

Section: Introductionmentioning

confidence: 99%

“…We have previously shown that the calbindin-D 9K cDNA used here detected a single transcript of the expected size (0·6 kb) on Northern blots of placental mRNA (Glazier et al 1992(Glazier et al , 1995. To check the size of the renal calbindin-D 9K and calbindin-D 28K transcripts, a pool of poly-A enriched RNA (Pharmacia Biotech, St Albans, UK) was prepared from kidneys of the CP and DP groups at each gestational age and Northern blots prepared as described previously (Glazier et al 1992).…”

Section: Rna Extraction and Northern/dot Blot Analysismentioning

confidence: 99%

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Altered calbindin mRNA expression and calcium regulating hormones in rat diabetic pregnancy

Hamilton¹,

Tein²,

Glazier³

et al. 2000

Journal of Endocrinology

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Offspring of rats with diabetes mellitus are at risk of reduced calcium and bone mineral content. Altered expression of the maternal calcium binding proteins, calbindin-D 9K and calbindin-D 28K , which are involved in renal and placental calcium transport, may underlie these problems.We have investigated the effect of diabetes on circulating concentrations of regulatory hormones with respect to calbindin-D mRNA concentrations. Three rat groups were studied; control (CP), streptozotocininduced diabetic (DP), and insulin-treated diabetic (DPI) pregnant rats. Calbindin-D 9K and calbindin-D 28K mRNA abundance in placenta and maternal kidney were measured at days 7, 15, 18 and 21 of gestation, together with serum or plasma concentrations of 1,25 dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ), parathyroid hormone (PTH), PTH-related protein (PTHrP), calcitonin, oestradiol and IGF-I. An increase in placental calbindin-D 9K mRNA abundance between days 18 and 21 in CP and DPI rats was severely blunted in the DP rats. In contrast, renal calbindin-D 28K mRNA abundance was greater at days 7, 15 and 18 in DP compared with CP rats, as was calbindin-D 9K at day 18. Calcitonin concentrations showed no differences between the groups, and both PTH and IGF-I were reduced over the first half of gestation, unlike the calbindins. In contrast, the concentrations of PTHrP and 1,25(OH) 2 D 3 were reduced at term in the DP group compared with the other two groups. Plasma oestradiol concentrations were lower in DP than in CP rats at days 7, 15 and 18, and most striking was the absence in DP rats of the peak of oestradiol seen at day 18 in CP rats. Despite the similarity between changes in placental calbindin mRNA and 1,25(OH) 2 D 3 , previous work has shown placental calbindin-D 9K regulation to be vitamin-D-independent. These studies produce suggestive evidence, therefore, that PTHrP and oestradiol may be involved in the altered calbindin-D expression by kidney and placenta in rat diabetic pregnancy.

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Section: 25(oh) 2 Dmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Rna Extraction and Northern/dot Blot Analysismentioning

confidence: 99%

See 1 more Smart Citation

Altered calbindin mRNA expression and calcium regulating hormones in rat diabetic pregnancy

Hamilton¹,

Tein²,

Glazier³

et al. 2000

Journal of Endocrinology

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show abstract

“…CaBP-d9k is expressed in a number of tissues, including duodenal absorptive epithelium (5), kidney (6), and syncytial trophoblast (7). CaBP-d28k was originally purified from chicken intestine (8) but is also expressed in kidney and throughout the nervous system (9,10) in species ranging from invertebrates to mammals (11).…”

mentioning

confidence: 99%

Endometrial calbindins are critical for embryo implantation: Evidence fromin vivouse of morpholino antisense oligonucleotides

Luu

Nie

Salamonsen

2004

Proc. Natl. Acad. Sci. U.S.A.

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The endometrium is receptive to embryo implantation only for a short period in each reproductive cycle: development of receptivity requires alterations in endometrial gene expression. Calbindin (CaBP)-d9k and CaBP-d28k are related proteins containing EF hand motifs that have a high affinity for Ca 2؉ . We previously demonstrated that endometrial expression of CaBP-d9k mRNA is highly regulated during implantation in the mouse. This project aimed to determine the temporal and spatial expression of both CaBP proteins during early pregnancy and to establish whether they are necessary for blastocyst implantation. CaBP-d28k protein, like CaBP-d9k, was up-regulated in the endometrial epithelium just before implantation but disappeared at implantation sites after attachment. By the judicious intrauterine injection of morpholino oligonucleotides (MO) against CaBP-d9k into WT and CaBP-d28k null mice just before implantation, we selectively eliminated one or both CaBPs from the uterine epithelium. Implantation was blocked only when both CaBP-d9k and CaBP-d28k were absent: treated WT mice and untreated CaBP-d28k null mice were fertile. Furthermore, the effect on implantation was highly dependent on the timing of injection of MO. This report examining the function of implantation-related genes in the uterus using MO demonstrates that this technique is a highly effective means to specifically target uterine proteins in vivo. This study provides evidence for an absolute requirement for CaBPs during the early phase of embryo implantation, and thus that regulation of Ca 2؉ availability in the uterine environment of the implanting embryo is critical for successful implantation.

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“…The Hannover pig model showed that the fetuses of homozygous 1,25(OH) 2 D-deficient sows also maintained completely normal blood calcium and phosphate levels and fully mineralized their skeletons 62 . Also, fetal mice that lack the gene encoding the VDR are born with normal skeletons [83][84][85] . Human studies have also shown that at term the cord blood calcium and skeletal mineralization is completely normal in fetuses of vitamin D-deficient mothers 86,87 .…”

Section: Vitamin D and The Regulation Of Mineral Homeostasis During Pmentioning

confidence: 99%

Vitamin D and Human Pregnancy

Grayson¹,

Hewison

2011

Fet. Matern. Med. Rev.

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At the end of 2007, Time magazine listed the “benefits of vitamin D” as one of its top 10 medical breakthroughs for that year. Since then there has been a remarkable upsurge of interest in vitamin D, with new research advances seemingly published on a weekly basis. In particular, there has been increasing awareness of the variability of vitamin D status in populations across the globe and, significantly, a growing debate about the need for revised parameters for vitamin D supplementation. Although sub-optimal vitamin D is likely to be a widespread problem for 21stcentury societies, it is also clear that some groups are at much greater risk of low vitamin D status. Prominent amongst these are pregnant women and the aim of the following review article will be to discuss this problem in further detail with specific emphasis on its potential physiological and clinical impact.

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Calbindin-D9K Gene Expression in Rat Chorioallantoic Placenta Is Not Regulated by 1,25-Dihydroxyvitamin D3

Cited by 45 publications

References 25 publications

Altered calbindin mRNA expression and calcium regulating hormones in rat diabetic pregnancy

Altered calbindin mRNA expression and calcium regulating hormones in rat diabetic pregnancy

Endometrial calbindins are critical for embryo implantation: Evidence fromin vivouse of morpholino antisense oligonucleotides

Vitamin D and Human Pregnancy

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