Calcareous Pericarditis in Mice of Several Genetically Related Strains

DiPaolo, Joseph A.; Strong, Leonell C.; Moore, George E.

doi:10.3181/00379727-115-28950

Cited by 28 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed severity of DCC in the DBAJ2 and C3H mice is in accordance with literature reports (DiPaolo et al 1964}. The observed absence of DCC in CS7BL and BALB/c mice also confirms earlier publications.…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

Broek¹,

Beems

Tintelen

et al. 1997

Lab Anim

View full text Add to dashboard Cite

SummaryDystrophic cardiac calcification (DCC) is a post-mortem finding in mice of various strains frequently used in biomedical research. The major aim of this study was to see whether DCC severity can be assessed by chemical analysis of calcium in the heart. Histological examination was used as the method of reference. Hearts of mice of four strains (BALB/c,C3H, C57BL/6 and DBA/2) were halved and the two resulting parts were subsequently subjected to histology or chemical analysis. Within hearts, the halves generally yielded similar results. The DCC scores and calcium contents were directly correlated within hearts. Thus, calcium analysis could serve as an alternative to histological examination in the assessment of DCC severity in mice.DBA/2 and C3H mice were found to be affected by DCC. Plasma magnesium concentrations were lower in these strains than in the DCC-free C57BL/6 and BALB/cstrains. The tongue, lungs and diaphragm were also found to be calcified in DCC positive animals. Possibly, DCC is just one component of a generalized soft tissue calcification.

show abstract

Section: Discussionsupporting

confidence: 81%

“…phosphorus Dystrophic cardiac calcification (DCC) is found in various inbred strains of mice frequently used in biomedical research (DiPaolo et al 1964). Usually, DCC is discovered at post-mortem examination of the heart of apparently healthy mice.…”

mentioning

confidence: 99%

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

Broek¹,

Beems

Tintelen

et al. 1997

Lab Anim

View full text Add to dashboard Cite

show abstract

“…Age-related spontaneous dystrophic cardiac calcinosis (DCC) occurs in several inbred strains of mice, including BALB/c, DBA/2, and C3H; DCC may even lead to congestive heart failure in older animals (9)(10)(11)(12). Apart from age and genetic background, other factors including infectious agents (13)(14)(15), sex (9,12), hormonal status (9,(16)(17)(18), and diet (1,9,(19)(20)(21) can markedly influence the time of onset and the severity of DCC.…”

Section: Introductionmentioning

confidence: 99%

A locus on chromosome 7 determines myocardial cell necrosis and calcification (dystrophic cardiac calcinosis) in mice.

Ivandic

Qiao

Machleder

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Calcium deposition at sites of inflammation and necrosis is a fundamental but poorly understood element of the response of tissue to injury. It is evident in clinical diseases, including atherosclerosis and cardiac valve sclerosis, in which chronic inflammation or degenerative process with cell death is involved. In the presence of normal calcium and phosphate serum concentrations, such calcification is usually termed dystrophic calcification or calcinosis. Ultrastructural studies have shown that the initial events include cell necrosis and granular calcium deposition in or around the mitochondria (1-3). The pivotal role of intracellular calcium concentrations for cell injury and necrosis has been established (4, 5) and recent data also suggest the involvement of cellular calcium homeostasis in the pathogenesis of chronic myocarditis and cardiomyopathy (6-8).Age-related spontaneous dystrophic cardiac calcinosis (DCC) occurs in several inbred strains of mice, including BALB/c, DBA/2, and C3H; DCC may even lead to congestive heart failure in older animals (9-12). Apart from age and genetic background, other factors including infectious agents (13-15), sex (9, 12), hormonal status (9,(16)(17)(18), and diet (1, 9, 19-21) can markedly influence the time of onset and the severity of DCC. The factors involved in the various etiologies of DCC are different, yet a common element of each is cell injury, necrosis, and subsequent calcium deposition. The typical pattern of susceptibility to DCC was also observed following a standardized myocardial freeze-thaw injury, suggesting a common genetic basis independent from the nature of the etiology (22).We now report the mapping of a major gene determining DCC, designated Dyscalc, on proximal mouse chromosome 7. The locus was identified by quantitative trait locus (QTL) analysis of an F2 intercross between the susceptible strain C3H/HeJ and the resistant strain C57BL/6J using a complete linkage map approach. The significance of the QTL results was tested by permutation analysis and the map position was further confirmed by analysis of a set of recombinant inbred (RI) strains derived from these progenitor strains. The results have implications for the understanding of cell injury and necrosis in myocardial and cardiovascular diseases. MATERIALS AND METHODSAnimals. All mice were obtained from The Jackson Laboratory. An F2 intercross between inbred strains C57BL/6J and C3H/HeJ was constructed in our laboratory; only female progeny (n = 197) were included in the studies to eliminate gender differences as a potential confounder. All animals were maintained in pathogen-free facilities on a 12-hr light/12-hr dark cycle with free access to water and food throughout the experimental period. At 3 months of age, mice were placed on a high-fat, high-cholesterol diet (TD 90221; Harlan-Teklad, Madison, WI) to accelerate DCC (23). After 8 weeks of high-fat diet, mice were sacrificed by cervical dislocation.Histological Analyses. After the mice were killed, the heart and proximal aorta were exci...

show abstract

“…Well known DCC-susceptible mouse strains are the DBA/2 and C3H strains, whereas the C57BL/6 strain is resistant (DiPaolo et al 1964, Van den Broek et al 1997.In the DBA/2 and C3H strains calcific deposits in the heart are often associated with cell death so that this condition is called DCC. Calcifications in other organs are not necessarily dystrophic although they are consistently associated with Dee (Doi et al 1985, Imaoka et al 1986).…”

Section: Correspondence To: F a R Van Den Broekmentioning

confidence: 99%

The influence of dietary phosphorus and magnesium concentrations on the calcium content of heart and kidneys of DBA/2 and NMRI mice

Broek

Beynen

1998

Lab Anim

View full text Add to dashboard Cite

SummaryDystrophic cardiac calcification (DCC) is often found in DBA/2 mice, reportedly in association with low plasma magnesium levels in this mouse strain. It was hypothesized that high-phosphorus diets and low-magnesium diets that are known to lower plasma magnesium concentrations would promote the development of DCC. DBA/2 mice were fed diets with either low-magnesium (0.02%, w/wl or high-phosphorus (0.8%) concentrations or a combination of the two variables. NMRI mice were given either a low-(0.2%) or high-(0.6%) phosphorus diet.Female, but not male, NMRI mice accumulated calcium in the heart when fed the highphosphorus diet; neither gender developed kidney calcification. DBA/2 mice with either a low-magnesium or a high-phosphorus intake developed marked cardiac calcifications. The combination of low-magnesium and high-phosphorus intake caused severe calcification of the heart, kidney and tongue'. Increasing the dietary magnesium content (0.08%) and reducing phosphorus (0.2%) did not fully prevent cardiac calcification, but reduced heart calcium concentrations in male DBA/2 mice. It is concluded that diets for DCC-susceptib1e mice should contain adequate amounts of magnesium and low, but sufficient amounts of phosphorus in order not to additionally stimulate cardiac calcification.

show abstract

Calcareous Pericarditis in Mice of Several Genetically Related Strains

Cited by 28 publications

References 0 publications

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

Co-variance of chemically and histologically analysed severity of dystrophic cardiac calcification in mice

A locus on chromosome 7 determines myocardial cell necrosis and calcification (dystrophic cardiac calcinosis) in mice.

The influence of dietary phosphorus and magnesium concentrations on the calcium content of heart and kidneys of DBA/2 and NMRI mice

Contact Info

Product

Resources

About