Calcein-Modified Multinanochannels on PET Films for Calcium-Responsive Nanogating

Abstract

Calcein-modified multiporous films with conical channels are introduced in a nanofluid device to enhance the calcium-responsive intensity and stability of ionic currents. Calcein with more carboxyls enhances the response of channels to calcium ions, and the capability of immobilized calcein for Ca(2+)-binding could be regulated by the deprotonation of these carboxyls.

Fundamental Studies of Nanofluidics: Nanopores, Nanochannels, and Nanopipets

“…Calcein-modified channels bind calcium ions, which reduce conductance in the high-conductance state. 105 Similarly, lactoferrin is also sensed by nanopores modified with iron-terpyridine. 106 PET pores modified with β-cyclodextrin discriminate between enantiomers of histidine.…”

Conductivity-based detection techniques in nanofluidic devices

“…Ca 2+ is also detected with calcein-modified PET pores that exhibit a lower RR in the presence of Ca 2+ at high pH. 141 …”

“…Ca 2+ is also detected with calcein-modified PET pores that exhibit a lower RR in the presence of Ca 2+ at high pH. 141 Pores with asymmetric geometries that exhibit ICR can also be used to study small molecules. ICR increases cation concentration within a polymer nanopore above the bulk solubility product to precipitate CoHPO 4 and CaHPO 4 , demonstrating the ability of nanopores to probe kinetics and early stages of crystallization.…”

Biomimetic Solid-State Nanochannels: From Fundamental Research to Practical Applications

“…Inspired by these biological nanochannels, a series of sensors have been developed rapidly in recent years based on modified biomimetic smart nanochannels, which have the potential to be used in mimicking specific identification processes in cell membranes. In previous work, many sensors with cation ions specific identification, such as silver ion, lead ion, calcium ion, potassium ion, sodium ion,[6a] zinc ion, mercury ion, copper ion, and even glucose, have been fabricated.…”