Calcification of degenerating tissues in the periodontal ligament during tooth movement

Nakamura, Yoshiki; Tanaka, Takashi; Noda, Koji; Shimpo, Satoshi; Oikawa, Takashi; Hirashita, Ayao; Kawamoto, Tadafumi; Kawasaki, Kenzo

doi:10.1034/j.1600-0765.2003.00671.x

Cited by 19 publications

(13 citation statements)

References 7 publications

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“…Group 2 consisted of 5 rats, and they wore orthodontic wire (experimental group). Upper first molars of the experiment groups were moved palatally (0.1 N) with the fixed appliance (Nakamura et al, 2003). …”

Section: Methodsmentioning

confidence: 99%

“…Cells in the PDL are subjected to continuous mechanical strain and are forced to adapt to the new environment by synthesis and secretion of several cytokines and growth factors (Arai et al, 2010; Baba et al, 2011; Saito et al, 1991; Tsuge et al, 2016). As a result, reconstruction of the PDL and alveolar bone occur both in the tension and compression zones of the PDL (Nakamura et al, 2003; Shimpo et al, 2003; Takahashi et al, 2003, 2006). However, the detailed mechanisms of the cellular response in reconstruction of the PDL and alveolar bone have not been clarified.…”

Section: Introductionmentioning

confidence: 99%

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Novel device for application of continuous mechanical tensile strain to mammalian cells

et al. 2017

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During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tensile strain to cells in culture. The continuous tensile strain was applied to human immortalized periodontal ligament cell line (HPL cells) and the cytoskeletal structures of HPL cells were examined by immunohistochemistry. The expression of both inflammatory and osteogenic markers was also examined by real-time reverse transcription polymerase chain reaction. The osteogenic protein, Osteopontin (OPN), was also detected by western blot analysis. The actin filaments of HPL cells showed uniform arrangement under continuous tensile strain. The continuous tensile strain increased the expression of inflammatory genes such as IL-1β, IL-6, COX-2 and TNF-α, and osteogenic genes such as RUNX2 and OPN in HPL cells. It also elevated the expression of OPN protein in HPL cells. These results suggest that our new simple device is useful for exploring the responses to continuous tensile strain applied to the cells.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel device for application of continuous mechanical tensile strain to mammalian cells

et al. 2017

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“…In this context, it has been suggested that ALP plays an important role in the supply of phosphoric acid in calcification (Mayahara 1980), or is a kind of calciumbinding protein (Bernard et al 1986). ALP in the periodontal ligament was localized in the plasma membrane of the fibroblasts and collagen fibers, but none of the periodontal ligament calcifies except in pathological conditions (Nakamura et al 2003). ALP in the fibroblasts and collagen fibers may regulate the local concentration of phosphate or calcium.…”

Section: Discussionmentioning

confidence: 98%

Phosphatidylinositol-dependent bond between alkaline phosphatase and collagen fibers in the periodontal ligament of rat molars

Nakamura

Noda

Shimpo

et al. 2004

Histochemistry and Cell Biology

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Alkaline phosphatase (ALP) is anchored to the outer leaflet of the lipid bilayer via phosphatidylinositol (PI) and ALP activity has been localized in the plasma membrane of numerous tissues. In the periodontal ligament ALP activity is found in the collagen fibers in addition to the plasma membrane of the osteoblasts and fibroblasts. In this study, we examined the distribution of ALP activity in the periodontal ligament of rat molars and also examined whether the bond between ALP and collagen fibers is dependent on PI by using phosphatidylinositol-specific phospholipase C (PI-PLC). ALP activity was distributed in the periodontal ligament. The activity mirrored the distribution of collagen fibers in the periodontal ligament. Cytochemical analysis also demonstrated that ALP activity was located not only in the plasma membrane of fibroblasts, but also in the collagen fiber bundles and fibrils in the periodontal ligament. After treatment with PI-PLC, the loss of ALP activity in the periodontal ligament was observed histochemically, and the loss of ALP activity in the fibroblasts as well as in the collagen fiber bundles and fibrils was observed cytochemically. These results strongly indicate that the bond between ALP and the collagen fibers is also dependent on PI.

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“…Continuous orthodontic force on the tooth is considered to be a kind of physical stress loaded to the periodontal ligament (PDL) (Macapanpan et al 1954;Reitan 1960;Kvam 1969;Azuma 1970;Rygh 1972Rygh , 1974Vandevska-Radunovic et al 1994;Nakamura et al 1996Nakamura et al , 2003. This stress causes gradual compression of the PDL, which leads to circulatory disturbance such as ischemia and hypoxia in the pressure zone of the PDL at early stages of tooth movement.…”

Section: Introductionmentioning

confidence: 98%

HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats

Arai

Nomura

Ishikawa

et al. 2010

Histochem Cell Biol

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Heat shock proteins (HSPs) are molecular chaperones that maintain intracellular protein homeostasis and ensure survival of cells. Continuous orthodontic force on the tooth is considered to be a type of physical stress loaded to the periodontal ligament (PDL). However, little is known about the role of HSPs during tooth movement. This study was performed to examine the expression of HSPs in the PDL during tooth movement using laser microdissection, microarray analysis, real-time RT-PCR and immunohistochemistry. Gene expression of HSPA1A in the pressure zone of the PDL was higher during 6 h of tooth movement than in the control group. Expression of HSPA1A decreased with time. HSPA1A was also detected in the pressure zone of the PDL at the protein level 24 h after the initial tissue change. These results strongly suggest that expression of HSPA1A in the PDL during early stages of tooth movement is a critical factor for tissue reaction.

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Calcification of degenerating tissues in the periodontal ligament during tooth movement

Abstract: This calcification of the degenerating tissues is a self-defense response of the living body to prevent direct contact between alveolar bone and the tooth root during compression of the PDL, so preventing friction between them and the development of ankylosis.

Cited by 19 publications

References 7 publications

Novel device for application of continuous mechanical tensile strain to mammalian cells

Novel device for application of continuous mechanical tensile strain to mammalian cells

Phosphatidylinositol-dependent bond between alkaline phosphatase and collagen fibers in the periodontal ligament of rat molars

HSPA1A is upregulated in periodontal ligament at early stage of tooth movement in rats

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