Calcineurin Contributes to the Enhancing Effect of Adenosine on Nerve Growth Factor-Induced Neurite Outgrowth via the Decreased Duration of p38 Mitogen-Activated Protein Kinase Phosphorylation

Muroi, Yoshikage; Ishii, Toshiaki; Teramoto, Kentarou; Hori, Masaru; Nishimura, Masakazu

doi:10.1254/jphs.95.124

Cited by 14 publications

(7 citation statements)

References 22 publications

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“…To determine whether purine nucleosides may attenuate hypoxic cell death, cells were exposed to hypoxia in the presence of adenosine and inosine. In agreement with results by others (Huffaker et al 1984;Braumann et al 1986;Gysbers and Rathbone 1996;Kobayashi and Millhorn 1999;Muroi et al 2004;Ribeiro 2005;Tomaselli et al 2005b), the data presented here demonstrate that adenosine and inosine efficiently rescue the viability of hypoxic PC12 cells and primary cerebellar granule neurons. Earlier data suggested that the effects of adenosine were apparently the result of its conversion to inosine by (a)…”

Section: Discussionsupporting

confidence: 93%

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HIF‐1 alpha is an essential effector for purine nucleoside‐mediated neuroprotection against hypoxia in PC12 cells and primary cerebellar granule neurons

Nedden

Tomaselli²,

Baier‐Bitterlich

2008

Journal of Neurochemistry

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Abbreviations used: A 1 R, A 2A R, A 2A R, A 3 R, adenosine receptors; BSA, bovine serum albumin; HIF, hypoxia-inducible factor; HRP, horseradish peroxidase; MAPK, mitogen-activated protein kinase; PBS, phosphate-buffered saline; PC12, clonal rat pheochromocytoma cells; TBS, Tris-buffered saline; TBS-T, Tris-buffered saline Tween-20; siRNA, small interfering RNA. AbstractHypoxia-inducible factor-1 alpha (HIF-1a) and purine nucleosides adenosine and inosine are critical mediators of physiological responses to acute and chronic hypoxia. The specific aim of this paper was to evaluate the potential role of HIF-1a in purine-mediated neuroprotection. We show that adenosine and inosine efficiently rescued clonal rat pheochromocytoma (PC12) cells (up to 43.6%) as well as primary cerebellar granule neurons (up to 25.1%) from hypoxic insult, and furthermore, that HIF-1a is critical for purine-mediated neuroprotection. Next, we studied hypoxia or purine nucleoside increased nuclear accumulation of HIF-1a in PC12 cells. As a possible result of increased protein stabilization or synthesis an up to 2.5-fold induction of HIF-1a accumulation was detected. In cerebellar granule neurons, purine nucleosides induced an up to 3.1-fold HIF-1a accumulation in cell lysates. Concomitant with these results, small interfering RNA-mediated reduction of HIF-1a completely abolished adenosine-and inosine-mediated protection in PC12 cells and severely hampered purine nucleoside-mediated protection in primary neurons (up to 94.2%). Data presented in this paper thus clearly demonstrate that HIF-1a is a key regulator of purine nucleoside-mediated rescue of hypoxic neuronal cells.

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Section: Discussionsupporting

confidence: 93%

“…1984; Braumann et al. 1986; Gysbers and Rathbone 1996; Kobayashi and Millhorn 1999; Muroi et al. 2004; Ribeiro 2005; Tomaselli et al.…”

Section: Discussionmentioning

confidence: 99%

HIF‐1 alpha is an essential effector for purine nucleoside‐mediated neuroprotection against hypoxia in PC12 cells and primary cerebellar granule neurons

Nedden

Tomaselli²,

Baier‐Bitterlich

2008

Journal of Neurochemistry

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“…The p38 MAP kinase pathway is also critically involved in NGFinduced neuronal differentiation of PC12 cells. [59][60][61][62] In our current study, p38 MAP kinase pathway was not involved in DA-9801-induced neurite outgrowth in PC12 cells (Fig. 4D).…”

Section: )mentioning

confidence: 43%

DA-9801 Promotes Neurite Outgrowth <i>via</i> ERK1/2-CREB Pathway in PC12 Cells

Won

Ahn

Back

et al. 2015

Biological & Pharmaceutical Bulletin

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“…While the phosphorylation of p38MAPK is necessary for neural differentiation [27, 28], the phosphorylation of p38MAPK inhibits this process [29]. Thus, the results above suggested that the phosphorylation of p38MAPK could promote or inhibit neurite outgrowth depending on the experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

Neurite Outgrowth in PC12 Cells Stimulated by Components fromDendranthema×grandiflorumcv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK

Nishina

Kimura

Tsukagoshi

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Components from Dendranthema × grandiflorum cv. “Mottenohoka” that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth.

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Calcineurin Contributes to the Enhancing Effect of Adenosine on Nerve Growth Factor-Induced Neurite Outgrowth via the Decreased Duration of p38 Mitogen-Activated Protein Kinase Phosphorylation

Cited by 14 publications

References 22 publications

HIF‐1 alpha is an essential effector for purine nucleoside‐mediated neuroprotection against hypoxia in PC12 cells and primary cerebellar granule neurons

HIF‐1 alpha is an essential effector for purine nucleoside‐mediated neuroprotection against hypoxia in PC12 cells and primary cerebellar granule neurons

DA-9801 Promotes Neurite Outgrowth <i>via</i> ERK1/2-CREB Pathway in PC12 Cells

Neurite Outgrowth in PC12 Cells Stimulated by Components fromDendranthema×grandiflorumcv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK

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