Calcineurin mediates the immune response of hemocytes through NF-κB signaling pathway in pearl oyster (Pinctada fucata)

Li, Changzhong; Liang, Jian; Ma, Zhuojun; Hu, Yilin; Yan, Zhenguang; Li, Qing; Fang, Zhujun; Wang, Hongzhong; Zhang, Guiyou; Xie, Liping; Zhang, Rongqing

doi:10.1016/j.fsi.2009.11.001

Cited by 16 publications

(8 citation statements)

References 46 publications

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“…Calmodulin can be associated with almost all cellular processes, including apoptosis, metabolism, inflammation and the immune response. Some immune responses such NF-κB signaling pathway in pearl oyster are regulated by calmodulin binding proteins such as calcineurin [53] and calmodulin was shown to be an important molecular determinant response to Perkinsus infection [54,55]. …”

Section: Resultsmentioning

confidence: 99%

mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

et al. 2013

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BackgroundThe Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have designed an oligo microarray capable of contributing to provide some clues on molecular response of the clam to Perkinsiosis.ResultsA database consisting of 41,119 unique transcripts was constructed, of which 12,479 (30.3%) were annotated by similarity. An oligo-DNA microarray platform was then designed and applied to profile gene expression in R. decussatus heavily infected by Perkinsus olseni. Functional annotation of differentially expressed genes between those two conditionswas performed by gene set enrichment analysis. As expected, microarrays unveil genes related with stress/infectious agents such as hydrolases, proteases and others. The extensive role of innate immune system was also analyzed and effect of parasitosis upon expression of important molecules such as lectins reviewed.ConclusionsThis study represents a first attempt to characterize Ruditapes decussatus transcriptome, an important marine resource for the European aquaculture. The trancriptome sequencing and consequent annotation will increase the available tools and resources for this specie, introducing the possibility of high throughput experiments such as microarrays analysis. In this specific case microarray approach was used to unveil some important aspects of host-parasite interaction between the Carpet shell clam and Perkinsus, two non-model species, highlighting some genes associated with this interaction. Ample information was obtained to identify biological processes significantly enriched among differentially expressed genes in Perkinsus infected versus non-infected gills. An overview on the genes related with the immune system on R. decussatus transcriptome is also reported.

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Section: Resultsmentioning

confidence: 99%

mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

et al. 2013

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“…In our study, the HcCNA and HcCNB transcripts are ubiquitously expressed in all examined tissues, including hemocytes. As derived from the study in P. fucata, CN mediates the immune response of hemocytes via activating NF-κB signaling pathway (Li et al, 2010b). The activated CN, possibly synergized with PKC, stimulated IKK, through which IκBα was phosphorylated, ubiquitinated and degraded by the proteasome complex to release it from NF-κB which consequently bind to the promotors of IL-2 and iNOS.…”

Section: Discussionmentioning

confidence: 99%

“…The activated CN, possibly synergized with PKC, stimulated IKK, through which IκBα was phosphorylated, ubiquitinated and degraded by the proteasome complex to release it from NF-κB which consequently bind to the promotors of IL-2 and iNOS. Both IL-2 and iNOS are important defenders in the innate immune system of pearl oyster to fight against the invading pathogens (Li et al, 2010b). Infection of V. parahaemolyticus in many marine animals such as crabs, shrimp, oysters, and abalone has resulted in various disease symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…In mollusks, CNA and CNB subunits have been identified in the pearl oyster, Pinctada fucata, and expression of CNA was detected in a number of tissues including the mantle, gills, adductor muscle, foot, digestive gland, and gonad (Li et al, 2009). Inhibition of CN in this species revealed that it plays pivotal roles in both shell formation and the mediation of the immune response of hemocytes (Li et al, 2010a;Li et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of calcineurin A and B genes in the abalone, Haliotis diversicolor, and their immune response role during bacterial infection

Buddawong

Asuvapongpatana

Senapin

et al. 2020

PeerJ

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Calcineurin (CN) is known to be involved in many biological processes, particularly, the immune response mechanism in many invertebrates. In this study, we characterized both HcCNA and HcCNB genes in Haliotis diversicolor, documented their expression in many tissues, and discerned their function as immune responsive genes against Vibrio parahaemolyticus infection. Similar to other mollusk CNs, the HcCNA gene lacked a proline-rich domain and comprised only one isoform of its catalytic unit, in contrast to CNs found in mammals. HcCNB was highly conserved in both sequence and domain architecture. Quantitative PCR and in situ hybridization revealed that the genes were broadly expressed and were not restricted to tissues traditionally associated with immune function. Upon infection of H. diversicolor with V. parahaemolyticus (a bacteria that causes serious disease in crustaceans and mollusks), both HcCNA and HcCNB genes were highly up-regulated at the early phase of bacterial infection. HcCNB was expressed significantly higher than HcCNA in response to bacterial challenge, suggesting its independent or more rapid response to bacterial infection. Together, the two CN genes are unique in their gene structure (particular HcCNA) and distribution in mollusk species and likely function as immune responsive genes along with many other genes that are enhanced in the early phase of V. parahaemolyticus infection in abalone.

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“…CaN is the target of the immunosupressants FK506 and CsA, and controls the production of many cytokines including IL-2 and TNF-␣ in the T-cell activation pathway [2][3][4]. Recent studies have demonstrated that CaN stimulates innate immune responses by activating the NF-B signaling pathway [5][6][7]. As the regulatory subunit of CaN, CnB is a conserved and essential protein [8].…”

Section: Introductionmentioning

confidence: 99%

Calcineurin subunit B is an immunostimulatory protein and acts as a vaccine adjuvant inducing protective cellular and humoral responses against pneumococcal infection

Guo

et al. 2011

Immunology Letters

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Calcineurin mediates the immune response of hemocytes through NF-κB signaling pathway in pearl oyster (Pinctada fucata)

Cited by 16 publications

References 46 publications

mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditapes decussatus: a functional approach to unravel host -parasite interaction

Characterization of calcineurin A and B genes in the abalone, Haliotis diversicolor, and their immune response role during bacterial infection

Calcineurin subunit B is an immunostimulatory protein and acts as a vaccine adjuvant inducing protective cellular and humoral responses against pneumococcal infection

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