Calcite Dissolution by Brevibacterium sp. SOTI06: A Futuristic Approach for the Reclamation of Calcareous Sodic Soils

Abstract

Assessing the ability of soil microorganisms to dissolute poorly soluble native calcite to supply Ca2+ is a new area to be explored in reclaiming sodic soils by supplying adequate Ca2+ and reducing the recurrent sodicity. Hence, the present study aimed to isolate a calcite dissolving bacteria (CDB) from calcareous sodic soils and to understand the mechanism of calcite dissolution. Of the 33 CDB isolates recovered from the calcareous sodic soils of Tamil Nadu (Coimbatore, Ramnad, and Trichy), 11 isolates were screened for calcite dissolution based on titratable acidity. 16S rRNA gene sequence analysis of the three best isolates viz., SORI09, SOTI05, and SOTI06 revealed 99% similarity to Bacillus aryabhattai, 100% to B. megaterium, and 93% to Brevibacterium sp., respectively. Among them, Brevibacterium sp. SOTI06 released more Ca2+ (3.6 g.l−1) by dissolving 18.6% of the native calcite. The spectral data of FTIR also showed reduction in the intensity of calcite (55.36–41.27) by the isolate at a wave number of 1636 cm−1 which confirmed the dissolution. Besides producing organic acids (gluconic acid and acetic acid), Brevibacterium sp. SOTI06 also produced siderophore (91.6%) and extracellular polysaccharides (EPS, 13.3 μg. ml−1) which might have enhanced the calcite dissolution.

Biochemical and cultural characteristics of mineral-solubilizing Acinetobacter sp. DDP346

“…0.0001 g/L, agar 15 g/L, pH 6.8) 배 지 [10], Deveze-Bruni agar (D-glucose 5 g/L, yeast extract 1.0 g/L, peptone 1.0 g/L, K 2 HPO 4 0.4 g/L, MgSO 4 0.01 g/L, NaCl 5 g/L, (NH 4 ) 2 SO 4 0.05 g/L, CaCO 3 5 g/L, agar 15 g/L, pH 6.8) 배지 [11], silicate agar (D-glucose 10 g/L, magnesium trisilicate 2.5 g/L, agar 15 g/L, pH 6.8) 배지 [12 Data are mean ± SD of at least three replicates Nitrogen fixation activity was determined by color change of NFB broth…”

Calcite dissolving bacteria from peanut (Arachis hypogaea) pegging zone influences soil calcium level

“…Calcinogenic bacteria can convert CO 2 into insoluble CaCO 3 (calcite) in presence of soluble calcium (Lüttge and Conrad, 2004). On the other hand, calcite dissolving bacteria (CDB) dissolute poorly soluble calcite to generate Ca 2+ (Jacobson and Wu, 2009;Sulu-Gambari, 2012;Tamilselvi et al, 2016). CDB have previously been identified in calcareous soils, farmland, animal waste and limestone quarries (Subrahmanyam et al, 2009;Rana et al, 2015;Tamilselvi et al, 2016).…”

“…On the other hand, calcite dissolving bacteria (CDB) dissolute poorly soluble calcite to generate Ca 2+ (Jacobson and Wu, 2009;Sulu-Gambari, 2012;Tamilselvi et al, 2016). CDB have previously been identified in calcareous soils, farmland, animal waste and limestone quarries (Subrahmanyam et al, 2009;Rana et al, 2015;Tamilselvi et al, 2016). Several mechanisms are proposed for CDB to dissolve calcite, including secreting citric acid, oxalic acid and sanazine pigment (Subrahmanyam et al, 2009;Rana et al, 2015).…”

“…Several mechanisms are proposed for CDB to dissolve calcite, including secreting citric acid, oxalic acid and sanazine pigment (Subrahmanyam et al, 2009;Rana et al, 2015). CDB can be selected from media containing 1% of calcite on which they form a clear zone or "halo" around the colonies (Subrahmanyam et al, 2009;Tamilselvi et al, 2016). Several commercial operations use CDB to remove calcite deposits from pipes and other unwanted calcite formations (Eroglu et al, 2012).…”

Comparison of the Antifungal Activity, Plant Growth Promoting Activity, and Mineral Solubilization Ability of Bacillus sp. Isolated from Rhizosphere Soils and Roots

“…미네랄은 식물의 생장과 발달에 필수적이나 토양 내에서 대부분 불용성의 형태로 존재하고 있어 식물이 이용하는 데 어려움이 있다 [17]. 이와 관련하여 식물은 토양 내 존재하는 PGPR을 활용하는데, PGPR은 유기산 등을 생성하여 불용 성 미네랄을 가용화함으로써 식물에 미네랄을 공급하여 식 물 생장에 도움을 주는 것으로 알려져 있다 [17,18]. 그 중에 서도 Bacillus sp.의 미네랄 가용화능과 관련한 연구들에 의 하면, 옥수수 생장 촉진과 수확량 증대 [19], 세포벽 강화와 질병 감소 [20] 등을 보고하였다.…”